BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,305,900	G→T	R46L (CGC→CTC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,305,900	0	G	T	100.0%	514.6 / NA	144	R46L (CGC→CTC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (75/69); total (75/69)

Only 100 of 145 total aligned reads displayed.

GAATGCTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTGGGC  >  NC_000913/1305766‑1306033

    gCTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGa                                                                                                                              >  2:961030/1‑140 (MQ=255)

    gCTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGa                                                                                                                              <  1:1238003/140‑1 (MQ=255)

    gCTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGa                                                                                                                              >  1:2476477/1‑140 (MQ=255)

     cTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGaa                                                                                                                             <  1:957948/140‑1 (MQ=255)

     cTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCGGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGaa                                                                                                                             <  2:2304764/140‑1 (MQ=255)

      tGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAg                                                                                                                            >  1:1430930/1‑140 (MQ=255)

      tGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAg                                                                                                                            >  1:649101/1‑140 (MQ=255)

      tGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACA‑‑‑GTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAgggg                                                                                                                         <  2:2428003/140‑1 (MQ=255)

        tAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGa                                                                                                                              <  2:2276364/136‑1 (MQ=255)

        tAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGa                                                                                                                              >  1:2276364/1‑136 (MQ=255)

        tAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAggg                                                                                                                          >  1:17302/1‑140 (MQ=255)

        tAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAggg                                                                                                                          <  1:2453362/140‑1 (MQ=255)

        tAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAggg                                                                                                                          >  1:2432373/1‑140 (MQ=255)

        tAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAggg                                                                                                                          >  1:2091573/1‑140 (MQ=255)

         aaCTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAgggg                                                                                                                         >  2:1502063/1‑140 (MQ=255)

         aaCTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGAAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAgggg                                                                                                                         <  1:1109176/140‑1 (MQ=255)

         aaCGGAAGGAAAAAAAGTCCCCCTGAAAAGCGCCGATCTAAAAGTGCAATTTGAAATAAAAGATGAAAACCTGGGGTTTTGGCGACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAgggg                                                                                                                         <  2:606167/140‑1 (MQ=255)

          aCTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGa                                                                                                                        <  1:916216/140‑1 (MQ=255)

              aaggaagAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACa                                                                                                                    <  2:1706819/140‑1 (MQ=255)

              aaggaagAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACa                                                                                                                    <  1:991629/140‑1 (MQ=255)

                ggaagAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACAtt                                                                                                                  >  2:1256808/1‑140 (MQ=255)

                  aagaaAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAg                                                                                                                >  2:425660/1‑140 (MQ=255)

                   agaaAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAgg                                                                                                               <  2:2091573/140‑1 (MQ=255)

                    gaaAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGgt                                                                                                              >  2:931394/1‑140 (MQ=255)

                    gaaAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGgt                                                                                                              >  1:70316/1‑140 (MQ=255)

                     aaaaGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGgtg                                                                                                             <  1:931394/140‑1 (MQ=255)

                      aaaGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGgtgt                                                                                                            >  2:436481/1‑140 (MQ=255)

                          tcctccTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTa                                                                                                        >  1:2329761/1‑140 (MQ=255)

                            ctcctTGAAATTGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAAc                                                                                                                     >  1:217749/1‑125 (MQ=255)

                            ctcctTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAGAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACAtt                                                                                                                  >  2:585143/1‑128 (MQ=255)

                            ctcctTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAgg                                                                                                      >  1:539288/1‑140 (MQ=255)

                                 tGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAAt                                                                                                 <  1:1502063/140‑1 (MQ=255)

                                  gAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATc                                                                                                >  1:606765/1‑140 (MQ=255)

                                  gAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATc                                                                                                <  2:633113/140‑1 (MQ=255)

                                    aaTCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATc                                                                                                <  2:810190/138‑1 (MQ=255)

                                    aaTCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCgg                                                                                              >  2:2067326/1‑140 (MQ=255)

                                      gcGCCTATCTAAAAGTGCGCTTTGAAATCAAAGAGAACAACCAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGa                                                                                            <  2:1319047/139‑1 (MQ=255)

                                         ccGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGCGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGGGGTAGGGGAATCGGGATGc                                                                                         >  1:1820862/1‑140 (MQ=255)

                                          cGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCg                                                                                        <  1:885913/140‑1 (MQ=255)

                                            aTCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGt                                                                                      <  1:1190415/140‑1 (MQ=255)

                                                 aaaGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTc                                                                                 <  1:1816420/140‑1 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGt                                                                                      <  2:1777958/129‑1 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:1241725/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:418642/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:715444/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  1:1259859/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:1589082/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:2183196/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:295639/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  2:909502/1‑140 (MQ=255)

                                                       cACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCtt                                                                           >  1:567430/1‑140 (MQ=255)

                                                        aCTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCttt                                                                          >  1:294405/1‑140 (MQ=255)

                                                        aCTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCttt                                                                          >  2:54914/1‑140 (MQ=255)

                                                        aCTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCt                                                                            <  1:795817/138‑1 (MQ=255)

                                                               aaTCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTcgcg                                                                   >  2:2129679/1‑140 (MQ=255)

                                                                ataaaaGATGGAAAAAAGTGGTTTGGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCgcgc                                                                  <  1:526076/137‑1 (MQ=255)

                                                                ataaaaGAGGGAAAACAGTGGTTCGGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCgcgc                                                                  <  2:1430930/137‑1 (MQ=255)

                                                                    aaGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTg                                                                         <  1:2189282/129‑1 (MQ=255)

                                                                    aaGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTg                                                                         >  2:2189282/1‑129 (MQ=255)

                                                                    aaGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                              >  1:994180/1‑140 (MQ=255)

                                                                     agagGGAAAACAGTGTTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                             <  2:905706/140‑1 (MQ=255)

                                                                     aGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                             <  1:436481/140‑1 (MQ=255)

                                                                                aGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTc                                                  >  1:2332601/1‑140 (MQ=255)

                                                                                    gTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAgg                                              <  2:2432373/140‑1 (MQ=255)

                                                                                     ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGc                                             >  1:576765/1‑140 (MQ=255)

                                                                                       cTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatcat                                                            <  2:11467/123‑1 (MQ=255)

                                                                                       cTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatcat                                                            >  1:11467/1‑123 (MQ=255)

                                                                                         ggCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgacc                                         <  1:1589082/140‑1 (MQ=255)

                                                                                          gCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccg                                        >  2:788859/1‑140 (MQ=255)

                                                                                          gCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccg                                        <  1:909502/140‑1 (MQ=255)

                                                                                           cAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                       <  2:17302/140‑1 (MQ=255)

                                                                                            aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccgac                                      >  1:240798/1‑140 (MQ=255)

                                                                                            aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccgac                                      >  1:2448617/1‑140 (MQ=255)

                                                                                             aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACg                                     <  2:1883623/140‑1 (MQ=255)

                                                                                               cgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGt                                   >  1:870067/1‑140 (MQ=255)

                                                                                               cgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGt                                   >  1:78071/1‑140 (MQ=255)

                                                                                               cgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGt                                   >  2:429450/1‑140 (MQ=255)

                                                                                                  cgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                                <  1:2019580/140‑1 (MQ=255)

                                                                                                  cgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                                <  1:617272/140‑1 (MQ=255)

                                                                                                  cgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                                <  2:2448617/140‑1 (MQ=255)

                                                                                                  cgAAAACGCTCAAAGCCGTCGATGGTGTCACCCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                                >  1:2149860/1‑140 (MQ=255)

                                                                                                    aaaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                              >  1:108612/1‑140 (MQ=255)

                                                                                                      aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTg                            <  2:711259/140‑1 (MQ=255)

                                                                                                       aCGCTCAAAGCCGTCGATCGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGACTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCAGTTTGGTCAAGGCGACCGACGGTCATGTTGc                           <  2:1820862/140‑1 (MQ=255)

                                                                                                        cGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                          <  2:1412487/140‑1 (MQ=255)

                                                                                                        cGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                          <  1:723741/140‑1 (MQ=255)

                                                                                                        cGCTCAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                          <  2:870067/140‑1 (MQ=255)

                                                                                                            cAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGt                      <  2:240798/140‑1 (MQ=255)

                                                                                                            cAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGt                      <  1:2129679/140‑1 (MQ=255)

                                                                                                            cAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGt                      <  1:429450/140‑1 (MQ=255)

                                                                                                            cAAAGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGt                      <  1:397996/140‑1 (MQ=255)

                                                                                                             aaaGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGtt                     <  2:78071/140‑1 (MQ=255)

                                                                                                              aaGCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa                    >  2:350411/1‑140 (MQ=255)

                                                                                                                gCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAgg                  <  2:2329761/140‑1 (MQ=255)

                                                                                                                gCCGTCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAgg                  <  2:539288/140‑1 (MQ=255)

                                                                                                                    tCGATGGTGTCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa              >  1:1253985/1‑140 (MQ=255)

                                                                                                                           tgtCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTACCTGGTTAGGTAAAGAGTTGc       >  1:1705685/1‑140 (MQ=255)

                                                                                                                             tCACTCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTg     <  2:519160/140‑1 (MQ=255)

                                                                                                                             tCACCCTTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTg     <  2:2149860/140‑1 (MQ=255)

                                                                                                                                ctctTCTCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTGGGc  >  2:1396766/1‑140 (MQ=255)

GAATGCTGTAACTGAAGGAAGAAAAGTCCTCCTTGAAATCGCCGATCTTAAAGTGCACTTTGAAATCAAAGATGGCAAACAGTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTTGCTGGGC  >  NC_000913/1305766‑1306033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`