Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,879,829	Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp	coding (115‑120/360 nt)	yeaR ←	DUF1971 domain‑containing protein YeaR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1879829 =	0 (0.000)	45 (0.890)	35/270	0.3	100%	coding (120/360 nt)	yeaR	DUF1971 domain‑containing protein YeaR
	?	NC_000913	2514274 =	NA (NA)					noncoding (2/1345 nt)	IS186	repeat region
*	?	NC_000913	= 1879834	0 (0.000)	57 (1.130)	35/268	0.3	100%	coding (115/360 nt)	yeaR	DUF1971 domain‑containing protein YeaR
	?	NC_000913	= 2515616	NA (NA)					noncoding (1344/1345 nt)	IS186	repeat region

GCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAAT  >  NC_000913/2514135‑2514411                                                                                                                                             |                                                                                                                                          gCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑Tc                                                                                                                                           <  1:873084/140‑1 (MQ=255)   cTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑Tccc                                                                                                                                         >  2:538673/1‑140 (MQ=255)     gaatgaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCAt                                                                                                                                       >  2:211675/1‑140 (MQ=255)     gaatgaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCAt                                                                                                                                       >  2:766736/1‑140 (MQ=255)     gaatgaaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCAt                                                                                                                                       <  1:512636/140‑1 (MQ=255)          aaGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAgcg                                                                                                                                  <  1:6714/140‑1 (MQ=255)           agagCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAgcgc                                                                                                                                 >  2:407605/1‑140 (MQ=255)            gagCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCt                                                                                                                                <  2:384085/140‑1 (MQ=255)            gagCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCt                                                                                                                                <  1:766736/140‑1 (MQ=255)            gagCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGA‑ATTATCCCATAAGCGCt                                                                                                                                <  2:701411/140‑1 (MQ=255)                aaGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAgcgc                                                                                                                                 <  1:297081/135‑1 (MQ=255)                aaGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAgcgc                                                                                                                                 >  2:297081/1‑135 (MQ=255)                   gTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTaa                                                                                                                         <  2:701627/140‑1 (MQ=255)                          ggTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTtgt                                                                                                                  <  1:97284/140‑1 (MQ=255)                                    ttCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                                                                                            >  1:346037/1‑136 (MQ=255)                                    ttCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTAtt                                                                                                            <  2:346037/136‑1 (MQ=255)                                        gTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa                                                                                                    >  1:876938/1‑140 (MQ=255)                                              aGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAt                                                                                              >  1:883711/1‑140 (MQ=255)                                                  ggTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTaa                                                                                          >  2:279870/1‑140 (MQ=255)                                                             ctACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGa                                                                               >  2:758487/1‑140 (MQ=255)                                                                   tGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         <  1:211675/140‑1 (MQ=255)                                                                   tGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         <  1:758487/140‑1 (MQ=255)                                                                   tGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         <  2:876938/140‑1 (MQ=255)                                                                     gTGAGTGTGCTGTCTGCGTCAATCGCAGCGCTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct                                                                       >  2:500288/1‑140 (MQ=255)                                                                     gTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct                                                                       >  2:322908/1‑140 (MQ=255)                                                                     gTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct                                                                       >  2:856114/1‑140 (MQ=255)                                                                     gTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct                                                                       <  2:205856/140‑1 (MQ=255)                                                                     gTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         <  1:311631/138‑1 (MQ=255)                                                                     gTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         >  2:311631/1‑138 (MQ=255)                                                                        aGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAc                                                                    <  2:883711/140‑1 (MQ=255)                                                                          tgtgCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGATATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGa                                                                  >  2:739113/1‑140 (MQ=255)                                                                           gtgCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAt                                                                 <  1:322908/140‑1 (MQ=255)                                                                                gTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTg                                                            <  1:538673/140‑1 (MQ=255)                                                                                    gCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         <  1:846130/123‑1 (MQ=255)                                                                                    gCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAct                                                                         >  2:846130/1‑123 (MQ=255)                                                                                     cGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                       <  1:279870/140‑1 (MQ=255)                                                                                     cGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                       <  2:524935/140‑1 (MQ=255)                                                                                     cGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGATATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                       <  1:739113/140‑1 (MQ=255)                                                                                      gTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGc                                                      >  1:339341/1‑140 (MQ=255)                                                                                               agcgCTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                                             <  1:500288/140‑1 (MQ=255)                                                                                               agcaCTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                                             >  2:570986/1‑140 (MQ=255)                                                                                                   cTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCat                                         <  1:407605/140‑1 (MQ=255)                                                                                                      gTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                       <  1:446069/123‑1 (MQ=255)                                                                                                      gTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAg                                                       >  2:446069/1‑123 (MQ=255)                                                                                                                aCCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCg                            >  1:393476/1‑140 (MQ=255)                                                                                                                aCCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCg                            <  2:339341/140‑1 (MQ=255)                                                                                                                  catacataAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaa                          <  1:856114/140‑1 (MQ=255)                                                                                                                  catacataAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaa                          <  1:588083/140‑1 (MQ=255)                                                                                                                    tacataAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaaga                        >  2:563808/1‑140 (MQ=255)                                                                                                                           aaaaGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGAt                 >  2:785106/1‑140 (MQ=40)                                                                                                                                   gggATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaag                         >  2:851495/1‑124 (MQ=37)                                                                                                                                   gggATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCgaag                         <  1:851495/124‑1 (MQ=35)                                                                                                                                          t‑tCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAGCGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAAt  >  2:450838/1‑140 (MQ=11)                                                                                                                                             |                                                                                                                                          GCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTGTAAGACCATACATAAAAAAGCCGGGGATAAT‑TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAAT  >  NC_000913/2514135‑2514411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.