Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	38,132	C→A	L328L (CTG→CTT)	caiB ←	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	38,132	0	C	A	100.0%	163.1 / NA	47	L328L (CTG→CTT)	caiB	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/24);  total (23/24)

TGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCG  >  NC_000913/37995‑38264                                                                                                                                          |                                                                                                                                     tgtgAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAg                                                                                                                                    >  1:812934/1‑140 (MQ=255) tgtgAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAg                                                                                                                                    >  2:779901/1‑140 (MQ=255) tgtgAGGGCATTCCGCGCCAGATTTGTCCGGGGCTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAg                                                                                                                                    >  1:665153/1‑140 (MQ=255)  gtgAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGt                                                                                                                                   >  1:47451/1‑140 (MQ=255)      gggCATTCCGCACCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCg                                                                                                                               >  2:566483/1‑140 (MQ=255)       ggCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCgg                                                                                                                              >  2:544832/1‑140 (MQ=255)           ttCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACg                                                                                                                          >  1:42958/1‑140 (MQ=255)            tCCGCACCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACgg                                                                                                                         <  1:566483/140‑1 (MQ=255)             ccGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCAGTATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGt                                                                                                                        <  2:665153/140‑1 (MQ=255)                cgcCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAg                                                                                                                     <  1:779901/140‑1 (MQ=255)                cgcCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAg                                                                                                                     <  2:332182/140‑1 (MQ=255)                                               tCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCa                                                                                      >  1:624841/1‑140 (MQ=255)                                               tCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCa                                                                                      >  2:491968/1‑140 (MQ=255)                                                  gCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAg                                                                                   <  1:113593/140‑1 (MQ=255)                                                  gCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAg                                                                                   <  1:221890/140‑1 (MQ=255)                                                             ggCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTAc                                                                        <  1:491968/140‑1 (MQ=255)                                                              gCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACt                                                                       <  2:254893/140‑1 (MQ=255)                                                                ccTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTc                                                                     >  1:363088/1‑140 (MQ=255)                                                                 cTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTcc                                                                    <  2:47451/140‑1 (MQ=255)                                                                 cTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTcc                                                                    <  2:363088/140‑1 (MQ=255)                                                                 cTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTcc                                                                    <  2:812934/140‑1 (MQ=255)                                                                    tGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCcgcg                                                                 <  2:624841/140‑1 (MQ=255)                                                                    tGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCcgcg                                                                 >  2:409409/1‑140 (MQ=255)                                                                    tGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCcgcg                                                                 <  1:172351/140‑1 (MQ=255)                                                                    tGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCcgcg                                                                 <  1:409409/140‑1 (MQ=255)                                                                    tGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCcgcg                                                                 >  2:172351/1‑140 (MQ=255)                                                                    tGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCcgcg                                                                 <  1:544832/140‑1 (MQ=255)                                                                         gTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGt                                                            >  2:707121/1‑140 (MQ=255)                                                                         gTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGt                                                            >  1:434425/1‑140 (MQ=255)                                                                            cGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTAtg                                                         >  2:699686/1‑140 (MQ=255)                                                                              aCCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTAtgtg                                                       >  1:436416/1‑140 (MQ=255)                                                                                         tttGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGccagccag                                            >  2:625874/1‑140 (MQ=255)                                                                                          ttGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGGTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAgg                                           <  2:42958/140‑1 (MQ=255)                                                                                            gCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCa                                         <  2:436416/140‑1 (MQ=255)                                                                                                     tgattgatTCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTtc                                <  1:707121/140‑1 (MQ=255)                                                                                                         tgattCGCGAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCtt                            >  2:436001/1‑140 (MQ=255)                                                                                                                 gAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGt                    <  1:181335/140‑1 (MQ=255)                                                                                                                 gAGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGt                    <  1:625874/140‑1 (MQ=255)                                                                                                                  aGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTg                   >  2:620371/1‑140 (MQ=255)                                                                                                                  aGCCACATACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTg                   <  2:434425/140‑1 (MQ=255)                                                                                                                         tACTGTGGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTa            >  1:212092/1‑140 (MQ=255)                                                                                                                            tgtgGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAgg         <  2:391021/140‑1 (MQ=255)                                                                                                                            tgtgGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAgg         <  1:699686/140‑1 (MQ=255)                                                                                                                              tgGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGc       >  1:564361/1‑140 (MQ=255)                                                                                                                              tgGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGc       >  2:651/1‑140 (MQ=255)                                                                                                                              tgGATTGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCACGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGc       >  1:821201/1‑140 (MQ=255)                                                                                                                                   tGCTTTCAAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCg  <  1:620371/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCG  >  NC_000913/37995‑38264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑