BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,736	C→G	P241A (CCG→GCG)	mdtA →	multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,736	0	C	G	100.0%	154.6 / NA	42	P241A (CCG→GCG)	mdtA	multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (21/21); total (21/21)

GCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCAC  >  NC_000913/2154602‑2154872

gCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGaa                                                                                                                                     >  2:564464/1‑140 (MQ=255)

     aTTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGa                                                                                                                                <  1:564464/140‑1 (MQ=255)

     aTTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGa                                                                                                                                >  2:133994/1‑140 (MQ=255)

           gCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGc                                                                                                                          <  1:381611/140‑1 (MQ=255)

                 gTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGt                                                                                                                    >  1:551309/1‑140 (MQ=255)

                 gTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATC‑CCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTa                                                                                                                   >  1:106174/1‑140 (MQ=255)

                       ggTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCa                                                                                                              >  1:519055/1‑140 (MQ=255)

                             gTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCa                                                                                                        <  2:619289/140‑1 (MQ=255)

                                          aGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGaaaa                                                                                           <  2:551309/140‑1 (MQ=255)

                                             ttGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCg                                                                                        <  1:592075/140‑1 (MQ=255)

                                                aTGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCg                                                                                        >  2:192216/1‑137 (MQ=255)

                                                aTGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCg                                                                                        <  1:192216/137‑1 (MQ=255)

                                                aTGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCtg                                                                                     >  2:652308/1‑140 (MQ=255)

                                                aTGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCtg                                                                                     >  2:676856/1‑140 (MQ=255)

                                                  gTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCtggt                                                                                   >  2:382967/1‑140 (MQ=255)

                                                      gTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTa                                                                               >  1:287521/1‑140 (MQ=255)

                                                       tAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAg                                                                              <  1:676856/140‑1 (MQ=255)

                                                       tAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAg                                                                              <  1:133994/140‑1 (MQ=255)

                                                            aaaTCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGcc                                                                         <  1:652308/140‑1 (MQ=255)

                                                               tctcCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTgg                                                                      >  2:391181/1‑140 (MQ=255)

                                                                 tcCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGa                                                                    <  2:519055/140‑1 (MQ=255)

                                                                  ccAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGAt                                                                   <  1:391181/140‑1 (MQ=255)

                                                                           aTACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCa                                                               >  1:613314/1‑135 (MQ=255)

                                                                           aTACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCa                                                               <  2:613314/135‑1 (MQ=255)

                                                                           aTACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAAc                                                          >  1:214990/1‑140 (MQ=255)

                                                                           aTACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAAc                                                          >  2:294107/1‑140 (MQ=255)

                                                                                accGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCgaa                                                     <  2:287521/140‑1 (MQ=255)

                                                                                  cGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCgaaga                                                   >  1:721954/1‑140 (MQ=255)

                                                                                               aTCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAgg                                      >  2:851661/1‑140 (MQ=255)

                                                                                                tCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGc                                     >  1:34433/1‑140 (MQ=255)

                                                                                                 cACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCa                                    <  1:294107/140‑1 (MQ=255)

                                                                                                  aCCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCAc                                   >  2:678909/1‑140 (MQ=255)

                                                                                                  aCCCAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCAc                                   <  2:721954/140‑1 (MQ=255)

                                                                                                   cccAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACg                                  <  2:34433/140‑1 (MQ=255)

                                                                                                     cAGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCt                                <  1:678909/140‑1 (MQ=255)

                                                                                                      aGACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTg                               >  1:564323/1‑140 (MQ=255)

                                                                                                       gACGCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGt                              <  1:596159/140‑1 (MQ=255)

                                                                                                          gCATCCTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTaa                           <  1:382967/140‑1 (MQ=255)

                                                                                                              ccTATCGATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCt                       >  1:280847/1‑140 (MQ=255)

                                                                                                                    gATTTAGTCTTTACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATaa                 >  1:412882/1‑140 (MQ=255)

                                                                                                                               tACCCTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCT‑GATAACCAAATCGATGc     <  2:106174/140‑1 (MQ=255)

                                                                                                                                   cTGGCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCAc  <  2:214990/140‑1 (MQ=255)

GCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTTAGTCTTTACCCTGCCGGAAAGCGATATCGCTACCGTAGTGCAGGCGCAGAAAGCCGGAAAACCGCTGGTGGTAGAAGCCTGGGATCGCACCAACTCGAAGAAATTAAGTGAAGGCACGCTGTTAAGTCTAGATAACCAAATCGATGCCAC  >  NC_000913/2154602‑2154872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`