Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,811	G→C	R274R (CGC→CGG)	ypdF ←	aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,811	0	G	C	100.0%	161.6 / NA	44	R274R (CGC→CGG)	ypdF	aminopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (27/17);  total (27/17)

TAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCAC  >  NC_000913/2505682‑2505945                                                                                                                                  |                                                                                                                                       tAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCAt                                                                                                                              >  1:45278/1‑140 (MQ=255)           ggCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACctg                                                                                                                    >  1:178849/1‑140 (MQ=255)                tAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGc                                                                                                               >  1:674341/1‑140 (MQ=255)                      cgtGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCa                                                                                                         >  2:190104/1‑140 (MQ=255)                       gtggtgTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCAc                                                                                                        >  1:375427/1‑140 (MQ=255)                          gtgtCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGc                                                                                                      <  1:787895/139‑1 (MQ=255)                          gtgtCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGc                                                                                                      >  2:787895/1‑139 (MQ=255)                               ccGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGc                                                                                                >  2:2528/1‑140 (MQ=255)                                    gTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATc                                                                                           <  2:381873/140‑1 (MQ=255)                                    gTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATc                                                                                           <  2:674341/140‑1 (MQ=255)                                     tGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAgcg                                                                                                           >  1:258815/1‑123 (MQ=255)                                     tGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAgcg                                                                                                           <  2:258815/123‑1 (MQ=255)                                             gcgGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGAtt                                                                                  <  1:686164/140‑1 (MQ=255)                                                gATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATtgct                                                                               <  1:2528/140‑1 (MQ=255)                                                 aTCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATtgctg                                                                              <  2:178849/140‑1 (MQ=255)                                                     tCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAg                                                                          <  1:196878/140‑1 (MQ=255)                                                         gAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCtgt                                                                      <  2:240410/140‑1 (MQ=255)                                                            cTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCt                                                                        <  2:809102/135‑1 (MQ=255)                                                            cTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCt                                                                        >  1:809102/1‑135 (MQ=255)                                                              tCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGc                                                                         <  1:117216/132‑1 (MQ=255)                                                              tCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGc                                                                         >  2:117216/1‑132 (MQ=255)                                                                aaTGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCGGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCa                                                               >  1:242539/1‑140 (MQ=255)                                                                  tGCCGATAGCGGGACCGGTGTTATGACCGAAATAGTCGCCATAACCCGCTTCTGTAAGGACCCGCCGCGCGGCAGCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAgg                                                             <  2:102729/140‑1 (MQ=255)                                                                       aTAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAAt                                                        >  2:467994/1‑140 (MQ=255)                                                                       aTAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAAt                                                        >  1:266830/1‑140 (MQ=255)                                                                            gTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGAt                                                   <  2:266830/140‑1 (MQ=255)                                                                            gTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGAt                                                   <  1:467994/140‑1 (MQ=255)                                                                              gACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAg                                                 >  2:530855/1‑140 (MQ=255)                                                                                         tGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAAcag                                      >  1:641641/1‑140 (MQ=255)                                                                                              gAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGt                                 >  2:369963/1‑140 (MQ=255)                                                                                              gAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGt                                 >  2:162487/1‑140 (MQ=255)                                                                                              gAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGt                                 >  2:650447/1‑140 (MQ=255)                                                                                                      cGCCATAACCTGCTTCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCg                         <  1:650447/140‑1 (MQ=255)                                                                                                                   ttCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCtt            >  2:407274/1‑140 (MQ=255)                                                                                                                   ttCTGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCtt            >  2:785076/1‑140 (MQ=255)                                                                                                                      tGTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGc         <  1:530855/140‑1 (MQ=255)                                                                                                                       gTAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGcc        >  1:557099/1‑140 (MQ=255)                                                                                                                        tAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCt                    <  1:384220/127‑1 (MQ=255)                                                                                                                        tAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCt                    >  2:384220/1‑127 (MQ=255)                                                                                                                        tAATGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCa       >  2:558558/1‑140 (MQ=255)                                                                                                                         aaTGACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCAt      >  2:183297/1‑140 (MQ=255)                                                                                                                            gACCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCa   <  1:190104/140‑1 (MQ=255)                                                                                                                             aCCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCTTTCAc  >  1:235956/1‑140 (MQ=255)                                                                                                                             aCCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCAc  >  1:835237/1‑140 (MQ=255)                                                                                                                             aCCCGCCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCAc  >  2:594511/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       TAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCAC  >  NC_000913/2505682‑2505945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑