Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,747	G→T	P96P (CCC→CCA)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,747	0	G	T	100.0%	118.8 / NA	35	P96P (CCC→CCA)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/17);  total (18/17)

ACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGC  >  NC_000913/2734612‑2734883                                                                                                                                        |                                                                                                                                         aCAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCATGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCg                                                                                                                                      <  1:363570/140‑1 (MQ=255)   aGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTa                                                                                                                                    >  1:107627/1‑140 (MQ=255)   aGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTa                                                                                                                                    <  1:362506/140‑1 (MQ=255)        aCCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCt                                                                                                                               >  2:655318/1‑140 (MQ=255)          cGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTAt                                                                                                                                   >  1:213899/1‑134 (MQ=255)          cGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTAt                                                                                                                                   <  2:213899/134‑1 (MQ=255)          cGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCtat                                                                                                                             >  1:830321/1‑140 (MQ=255)          cGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCtat                                                                                                                             >  1:387099/1‑140 (MQ=255)             ctctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGt                                                                                                                                     <  1:426205/129‑1 (MQ=255)             ctctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGt                                                                                                                                     >  2:426205/1‑129 (MQ=255)               ctTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAagag                                                                                                                        >  1:728172/1‑140 (MQ=255)                  ccAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCa                                                                                                                     <  2:261110/140‑1 (MQ=255)                              cgcACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGtttt                                                                                                         >  2:832689/1‑140 (MQ=255)                                    gTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCa                                                                                                   <  2:728172/140‑1 (MQ=255)                                     tCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCa                                                                                                   <  1:449496/139‑1 (MQ=255)                                     tCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCa                                                                                                   >  2:449496/1‑139 (MQ=255)                                     tCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCAt                                                                                                  >  1:211022/1‑140 (MQ=255)                                       cACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATaa                                                                                                >  1:568880/1‑140 (MQ=255)                                             agccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCg                                                                                          >  1:544776/1‑140 (MQ=255)                                               ccagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGcc                                                                                        >  1:783182/1‑140 (MQ=255)                                                cagcATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCa                                                                                       <  1:655318/140‑1 (MQ=255)                                                   cATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTg                                                                                    <  2:783182/140‑1 (MQ=255)                                                   cATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTg                                                                                    <  2:312717/140‑1 (MQ=255)                                                      gAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGc                                                                                 <  2:604528/140‑1 (MQ=255)                                                        gcggcggcgACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCtt                                                                               >  2:83494/1‑140 (MQ=255)                                                                  cTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCt                                                                     <  2:211022/140‑1 (MQ=255)                                                                   ttCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCATACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCtt                                                                    <  2:387099/140‑1 (MQ=255)                                                                   ttCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCtt                                                                    <  2:544776/140‑1 (MQ=255)                                                                             gCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCGAACCGTGCCTGGCGTATTGCTATAAGAGGCTTCCGCCCGATTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTAc                                                          <  2:130952/140‑1 (MQ=255)                                                                             gCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTAc                                                          <  2:107627/140‑1 (MQ=255)                                                                                  aTTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt                                                     <  1:832689/140‑1 (MQ=255)                                                                                              cacaGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt                                                     >  2:329869/1‑128 (MQ=255)                                                                                              cacaGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCt                                                     <  1:329869/128‑1 (MQ=255)                                                                                              cacaGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCg                                         >  1:269776/1‑140 (MQ=255)                                                                                                   ggAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTa                                    >  1:759758/1‑140 (MQ=255)                                                                                                                                     gCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGc  >  1:750867/1‑140 (MQ=255)                                                                                                                                     gCCTGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGc  >  1:752194/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         ACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCGGCGGCGACTTCCGTTCCCGCTCGATTGCAAAACAGCACAGGGAGGCAGTCGGCAGTCATCACTGCGCAAACCGTGCCGGGCGTATTGCTATAAGAGGCATCCGCCCGTTTTGAGGCATAAGGTTCGCCAGTGAGCTTAAGCACATCTTTGCCGTGTACCTGCTCAAGCCAGACCGGTTTAGAAGGCAAATTGCCCGCAGCAAAAAGTCGCTTGC  >  NC_000913/2734612‑2734883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑