Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,505,167	C→A	G370G (GGC→GGA)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,505,167	0	C	A	100.0%	166.8 / NA	48	G370G (GGC→GGA)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/21);  total (27/21)

TTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGT  >  NC_000913/4505033‑4505303                                                                                                                                       |                                                                                                                                         ttggttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAgg                                                                                                                                     >  1:871133/1‑140 (MQ=255)   ggttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtg                                                                                                                                   <  2:820456/140‑1 (MQ=255)   ggttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCCTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtg                                                                                                                                   >  2:189435/1‑140 (MQ=255)      tCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGttt                                                                                                                                >  1:742741/1‑140 (MQ=255)           aaTTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCatta                                                                                                                           >  1:348921/1‑140 (MQ=255)             tttATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCattatt                                                                                                                         <  2:742741/140‑1 (MQ=255)              ttATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTc                                                                                                                        <  1:430015/140‑1 (MQ=255)                     tGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 <  2:301332/140‑1 (MQ=255)                           cTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTg                                                                                                           <  1:317345/140‑1 (MQ=255)                           cTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTg                                                                                                           <  1:728808/140‑1 (MQ=255)                              tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 >  1:165147/1‑131 (MQ=255)                              tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 <  2:401850/131‑1 (MQ=255)                              tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 <  2:165147/131‑1 (MQ=255)                              tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 >  1:401850/1‑131 (MQ=255)                              tCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGACCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCAt                                                                                                        >  2:151032/1‑140 (MQ=255)                                 aacaaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATtct                                                                                                     <  1:258196/140‑1 (MQ=255)                                   caaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTctct                                                                                                   <  2:348921/140‑1 (MQ=255)                                     aaaTTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGTGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGc                                                                                                 <  2:511071/140‑1 (MQ=255)                                                 ttAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAtttt                                                                                     >  1:454090/1‑140 (MQ=255)                                                      gACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAg                                                                                <  2:454090/140‑1 (MQ=255)                                                       aCCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGt                                                                               <  2:835196/140‑1 (MQ=255)                                                         cGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTg                                                                             >  1:834675/1‑140 (MQ=255)                                                          ggTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTgg                                                                            >  2:243846/1‑140 (MQ=255)                                                           gTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGt                                                                           <  2:871133/140‑1 (MQ=255)                                                                      aaCTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                >  2:666161/1‑140 (MQ=255)                                                                      aaCTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                >  1:112707/1‑140 (MQ=255)                                                                           aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGa                                                                      <  2:590060/129‑1 (MQ=255)                                                                           aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGa                                                                      >  1:590060/1‑129 (MQ=255)                                                                           aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                           >  1:181880/1‑140 (MQ=255)                                                                           aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                           >  1:836403/1‑140 (MQ=255)                                                                            ggggCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTaa                                                          <  1:189435/140‑1 (MQ=255)                                                                               gCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAAt                                                       >  2:447666/1‑140 (MQ=255)                                                                                  aCTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTta                                                    >  1:598308/1‑140 (MQ=255)                                                                                            tGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATcc                                          >  1:434673/1‑140 (MQ=255)                                                                                                   aCCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTa                                   >  1:492760/1‑140 (MQ=255)                                                                                                        tATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGa                              >  2:500401/1‑140 (MQ=255)                                                                                                           tAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTg                           <  1:500401/140‑1 (MQ=255)                                                                                                             gCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGa                         <  1:447666/140‑1 (MQ=255)                                                                                                               tacatacaTTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAgg                       >  1:321627/1‑140 (MQ=255)                                                                                                                  atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:315593/1‑140 (MQ=255)                                                                                                                  atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:404209/1‑140 (MQ=255)                                                                                                                  atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:292801/1‑140 (MQ=255)                                                                                                                   tacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCttt                   >  2:830459/1‑140 (MQ=255)                                                                                                                      atTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATg                <  1:292801/140‑1 (MQ=255)                                                                                                                           aGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaa           >  2:879360/1‑140 (MQ=255)                                                                                                                                attatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATg      >  1:437411/1‑140 (MQ=255)                                                                                                                                 ttatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATgg     <  1:494478/140‑1 (MQ=255)                                                                                                                                  tatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGc    <  2:437411/140‑1 (MQ=255)                                                                                                                                    tGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCgt  <  2:836403/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGT  >  NC_000913/4505033‑4505303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑