Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,715,375	(T)7→6	intergenic (‑55/‑48)	yfiE ← / → eamB	putative LysR‑type DNA‑binding transcriptional regulator YfiE/cysteine/O‑acetylserine exporter EamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,715,369	0	T	.	100.0%	147.2 / NA	35	intergenic (‑49/‑54)	yfiE/eamB	putative LysR‑type DNA‑binding transcriptional regulator YfiE/cysteine/O‑acetylserine exporter EamB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/21);  total (14/21)

TCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCT  >  NC_000913/2715230‑2715499                                                                                                                                            |                                                                                                                                   tCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcattcat                                                                                                                                    >  2:313722/1‑140 (MQ=255)        tgtACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCAttttttaa                                                                                                                             <  1:437075/140‑3 (MQ=255)             aGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGtt                                                                                                                       <  2:71011/140‑1 (MQ=255)               cacaATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTcc                                                                                                                     <  2:614764/140‑1 (MQ=255)               cacaATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTcc                                                                                                                     <  1:182124/140‑1 (MQ=255)               cacaATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTcc                                                                                                                     >  1:444655/1‑140 (MQ=255)                  aaTTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCgtg                                                                                                                  >  1:229688/1‑140 (MQ=255)                  aaTTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCgtg                                                                                                                  <  1:241232/140‑1 (MQ=255)                   aTTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCgtgt                                                                                                                 <  1:472899/140‑1 (MQ=255)                    tttttGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTc                                                                                                                >  2:504784/1‑140 (MQ=255)                         gCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTa                                                                                                           <  1:386903/140‑1 (MQ=255)                         gCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTa                                                                                                           <  1:9978/140‑1 (MQ=255)                            aaGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTg                                                                                                        <  2:346204/140‑1 (MQ=255)                             aGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGt                                                                                                       <  2:444655/140‑1 (MQ=255)                              gCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTg                                                                                                        <  2:243504/138‑1 (MQ=255)                              gCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTg                                                                                                        >  1:243504/1‑138 (MQ=255)                              gCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTc                                                                                                      <  2:263570/140‑1 (MQ=255)                                           aaCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACagag                                                                                         <  1:313722/140‑1 (MQ=255)                                            aCCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAgagg                                                                                        <  2:57130/140‑1 (MQ=255)                                                  ttCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATaa                                                                                  >  1:259525/1‑140 (MQ=255)                                                    ccaccaCGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACa                                                                                >  1:433498/1‑140 (MQ=255)                                                          cGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGaa                                                                          >  2:387038/1‑139 (MQ=255)                                                            gTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGacac                                                                        >  1:112216/1‑140 (MQ=255)                                                                 aaGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGAcc                                                                   <  1:504784/140‑1 (MQ=255)                                                                                                 cAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGAt                                   <  1:387038/140‑1 (MQ=255)                                                                                                  aaaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGTTTTTTGGACTTACACCCTGAtt                                  >  1:217842/1‑140 (MQ=255)                                                                                                   aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACtt                                             >  1:118/1‑128 (MQ=255)                                                                                                   aaCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACtt                                             <  2:118/128‑1 (MQ=255)                                                                                                    aCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTAc                                <  2:229688/140‑1 (MQ=255)                                                                                                        aTCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGCGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCt                            >  1:168815/1‑140 (MQ=255)                                                                                                         tCGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTa                           >  1:345806/1‑140 (MQ=255)                                                                                                          cGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTAt                          <  2:112216/140‑1 (MQ=255)                                                                                                          cGAAAATATCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTAt                          <  2:433498/140‑1 (MQ=255)                                                                                                                tatCGAATCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGcc                    >  1:236495/1‑140 (MQ=255)                                                                                                                       tCTAGAATATAAAAACATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCg             >  1:552900/1‑140 (MQ=255)                                                                                                                                 aaaaaCATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATtc   <  1:237547/140‑1 (MQ=255)                                                                                                                                  aaaaCATTCA‑TTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATtct  <  2:153566/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                   TCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCT  >  NC_000913/2715230‑2715499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑