Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,680	G→T	T188N (ACT→AAT)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,680	0	G	T	100.0%	157.2 / NA	45	T188N (ACT→AAT)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/21);  total (24/21)

CCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATACCG  >  NC_000913/1742543‑1742814                                                                                                                                          |                                                                                                                                       ccTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAttt                                                                                                                                      >  2:618084/1‑140 (MQ=255)    tCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTtgt                                                                                                                                   <  1:618084/140‑1 (MQ=255)         tGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATc                                                                                                                              <  2:621331/140‑1 (MQ=255)           gCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGa                                                                                                                            >  1:469177/1‑140 (MQ=255)              cGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGACCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGAttt                                                                                                                         <  2:399034/140‑1 (MQ=255)                         aTTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGAttt                                                                                                                         <  2:16665/129‑1 (MQ=255)                         aTTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGAttt                                                                                                                         >  1:16665/1‑129 (MQ=255)                                  cccccTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCa                                                                                                     >  1:628600/1‑140 (MQ=255)                                  cccccTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCa                                                                                                     >  1:443875/1‑140 (MQ=255)                                    cccTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAg                                                                                                   >  2:67058/1‑140 (MQ=255)                                                gCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTccc                                                                                       <  1:167915/140‑1 (MQ=255)                                                 cGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCa                                                                                      <  1:236703/140‑1 (MQ=255)                                                   aTTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAag                                                                                    >  2:165639/1‑140 (MQ=255)                                                     ttCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAagag                                                                                  <  2:267800/140‑1 (MQ=255)                                                     ttCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAagag                                                                                  <  2:170890/140‑1 (MQ=255)                                                             aGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGt                                                                          >  1:571176/1‑140 (MQ=255)                                                               gCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCACCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTc                                                                        >  1:612018/1‑140 (MQ=255)                                                                cTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTcc                                                                       <  1:362774/140‑1 (MQ=255)                                                                    tgtgCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAgtg                                                                   >  1:587946/1‑140 (MQ=255)                                                                        ccTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTc                                                               <  2:443875/140‑1 (MQ=255)                                                                           ggTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCgg                                                            >  2:592773/1‑140 (MQ=255)                                                                             ttGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGaa                                                          >  2:122257/1‑140 (MQ=255)                                                                                aTTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTa                                                       >  2:506846/1‑140 (MQ=255)                                                                                 ttCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTaa                                                      <  2:518773/140‑1 (MQ=255)                                                                                          ttttCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCa                                             >  1:389571/1‑140 (MQ=255)                                                                                             tctcGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCaaaa                                          >  1:91626/1‑140 (MQ=255)                                                                                                 gTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACTCAAAAAcgc                                      <  2:612018/140‑1 (MQ=255)                                                                                                 gTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAAcgc                                      <  2:587946/140‑1 (MQ=255)                                                                                                  tGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAAcgcg                                     <  2:571176/140‑1 (MQ=255)                                                                                                  tGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAAcgcg                                     <  2:628600/140‑1 (MQ=255)                                                                                                  tGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAAcgcg                                     >  1:178126/1‑140 (MQ=255)                                                                                                                        gTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               <  2:91626/140‑1 (MQ=255)                                                                                                                        gTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               <  1:122257/140‑1 (MQ=255)                                                                                                                        gTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               <  2:11054/140‑1 (MQ=255)                                                                                                                        gTATCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               <  1:165639/140‑1 (MQ=255)                                                                                                                          aTCTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCa             >  1:49785/1‑140 (MQ=255)                                                                                                                            cTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               <  2:441929/136‑1 (MQ=255)                                                                                                                            cTACCACTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               >  1:441929/1‑136 (MQ=255)                                                                                                                                 aCTGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAAt      >  1:502439/1‑140 (MQ=255)                                                                                                                                   tGCCTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGCTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATAc    >  1:600100/1‑140 (MQ=255)                                                                                                                                   tGCCTGATTTTGTGGATCGATTTCAATGTTGACGCATGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               <  2:400260/129‑1 (MQ=255)                                                                                                                                   tGCCTGATTTTGTGGATCGATTTCAATGTTGACGCATGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt               >  1:400260/1‑129 (MQ=255)                                                                                                                                     ccTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCGGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATACCg  >  2:619086/1‑140 (MQ=255)                                                                                                                                     ccTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATACCg  >  1:476961/1‑140 (MQ=255)                                                                                                                                     ccTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATACCg  >  1:391937/1‑140 (MQ=255)                                                                                                                                     ccTGATTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATACCg  >  1:191840/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGTCAGGCTAATACCG  >  NC_000913/1742543‑1742814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑