Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	96,565	C→A	G188G (GGC→GGA)	mraY →	phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	96,565	0	C	A	100.0%	125.5 / NA	37	G188G (GGC→GGA)	mraY	phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/19);  total (18/19)

TCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACC  >  NC_000913/96432‑96693                                                                                                                                      |                                                                                                                                 tCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAAcgcg                                                                                                                            <  2:198251/140‑1 (MQ=255)    ccTTCGCCCTGTACCTTGCCGGCAAAGACATGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTa                                                                                                                         <  2:160421/140‑1 (MQ=255)    ccTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTa                                                                                                                         <  2:393327/140‑1 (MQ=255)       tCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAAc                                                                                                                      >  2:128763/1‑140 (MQ=255)       tCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAAc                                                                                                                      >  1:294713/1‑140 (MQ=255)       tCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAAc                                                                                                                      >  2:203565/1‑140 (MQ=255)       tCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAAc                                                                                                                      >  1:459030/1‑140 (MQ=255)           ccTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTTCATTCTGCTGGCTTTCTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGa                                                                                                                  <  2:300293/140‑1 (MQ=255)              gtgccTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCTCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTGCATTCTGCTGGCTTTCTTCGTCATTGTGTGTACTGGAAACGCGGTAAACCTgtcgg                                                                                                               <  2:405779/137‑5 (MQ=255)                        ggCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGa                                                                                                     <  1:634816/140‑1 (MQ=255)                         gCAAAGACACGCCCGCAAGGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGAGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGGACTGGAAACGCGGTAAACCTGACCGATGGTCTCGAc                                                                                                    >  2:130533/1‑140 (MQ=255)                            aaGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGc                                                                                                 <  1:436694/140‑1 (MQ=255)                                cacGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTgg                                                                                             <  1:308832/140‑1 (MQ=255)                                cacGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTgg                                                                                             <  2:467678/140‑1 (MQ=255)                                         aaCGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGc                                                                                    <  2:294713/140‑1 (MQ=255)                                          aCGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGcc                                                                                   <  2:378188/140‑1 (MQ=255)                                          aCGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGcc                                                                                   <  2:57036/140‑1 (MQ=255)                                               gCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCg                                                                              <  1:373539/140‑1 (MQ=255)                                                      gtCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGt                                                                       <  1:203565/140‑1 (MQ=255)                                                        cccATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCg                                                                     >  2:105884/1‑140 (MQ=255)                                                             tCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGGGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCggt                                                                >  2:288272/1‑140 (MQ=255)                                                                       tgtgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTgcgc                                                      >  1:372170/1‑140 (MQ=255)                                                                         tgATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCtg                                                    >  1:300476/1‑140 (MQ=255)                                                                                       gggCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGAc                                      >  2:603629/1‑140 (MQ=255)                                                                                             ttCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGACaa                                >  2:649888/1‑140 (MQ=255)                                                                                                       tgctgGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACttt                      >  1:299270/1‑140 (MQ=255)                                                                                                         ctgGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGc                    >  1:598158/1‑140 (MQ=255)                                                                                                         ctgGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGc                    >  2:218789/1‑140 (MQ=255)                                                                                                         ctgGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGc                    >  2:503921/1‑140 (MQ=255)                                                                                                         ctgGCTTACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGc                    >  1:158181/1‑140 (MQ=255)                                                                                                               tACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa              <  2:383343/140‑1 (MQ=255)                                                                                                               tACTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTa              <  2:459030/140‑1 (MQ=255)                                                                                                                 cTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt            >  1:253319/1‑140 (MQ=255)                                                                                                                 cTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt            <  2:483871/140‑1 (MQ=255)                                                                                                                 cTTCGTCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACt            <  1:69442/140‑1 (MQ=255)                                                                                                                      tCATTGTGGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCAACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCat       >  1:629610/1‑140 (MQ=255)                                                                                                                           gtgGGTACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGACAATATGAACTTTGCCAGCTACTTGCATATAcc  <  1:649888/140‑1 (MQ=255)                                                                                                                                tACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCa        >  2:79082/1‑129 (MQ=255)                                                                                                                                tACTGGAAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCa        <  1:79082/129‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 TCGCCTTCGCCCTGTACCTTGCCGGCAAAGACACGCCCGCAACGCAGCTGGTGGTCCCATTCTTTAAAGATGTGATGCCGCAGCTGGGGCTGTTCTACATTCTGCTGGCTTACTTCGTCATTGTGGGTACTGGCAACGCGGTAAACCTGACCGATGGTCTCGACGGCCTGGCAATTATGCCGACCGTATTTGTCGCCGGTGGTTTTGCGCTGGTGGCGTGGGCGACCGGCAATATGAACTTTGCCAGCTACTTGCATATACC  >  NC_000913/96432‑96693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑