Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,629,867	G→T	A26S (GCT→TCT)	yfgH →	lipoprotein YfgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,629,867	0	G	T	100.0%	172.2 / NA	51	A26S (GCT→TCT)	yfgH	lipoprotein YfgH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/27);  total (24/27)

TTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCA  >  NC_000913/2629730‑2629998                                                                                                                                              |                                                                                                                                    ttACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCATATTCt                                                                                                                                   >  1:443210/1‑140 (MQ=255)      ttCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGa                                                                                                                              >  2:188291/1‑140 (MQ=255)      ttCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGa                                                                                                                              >  1:27465/1‑140 (MQ=255)         ctctTTTGATGATCTTAAACGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCa                                                                                                                           >  2:447275/1‑140 (MQ=255)          tctTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCAt                                                                                                                          <  2:540042/140‑1 (MQ=255)              ttGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCAtgctg                                                                                                                      >  1:594830/1‑140 (MQ=255)               tgatgaTCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCAtgctgc                                                                                                                     <  2:310867/140‑1 (MQ=255)                   gatCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGAt                                                                                                                 <  1:62412/140‑1 (MQ=255)                   gatCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGAt                                                                                                                 <  2:594830/140‑1 (MQ=255)                   gatCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGAt                                                                                                                 <  2:443210/140‑1 (MQ=255)                             gTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCaaa                                                                                                       <  1:138723/140‑1 (MQ=255)                              tCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAAc                                                                                                      <  2:617432/140‑1 (MQ=255)                                ttATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCg                                                                                                    >  2:152411/1‑140 (MQ=255)                                ttATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCg                                                                                                    >  2:407020/1‑140 (MQ=255)                                  aTTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGAt                                                                                                  <  2:607701/140‑1 (MQ=255)                                        cGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTg                                                                                            >  2:252823/1‑140 (MQ=255)                                        cGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTg                                                                                            >  1:82574/1‑140 (MQ=255)                                                       aaGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGaa                                                                             >  1:319217/1‑140 (MQ=255)                                                         ggAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAAc                                                                           >  2:591186/1‑140 (MQ=255)                                                         ggAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAAc                                                                           >  1:248773/1‑140 (MQ=255)                                                         ggAATAGTGATGAAATGTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAAc                                                                           >  2:572585/1‑140 (MQ=255)                                                           aaTAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTa                                                                         >  1:143237/1‑140 (MQ=255)                                                            aTAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaa                                                                        <  1:142850/140‑1 (MQ=255)                                                             tAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaaa                                                                       <  1:217290/140‑1 (MQ=255)                                                              aGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaaaa                                                                      <  2:27465/140‑1 (MQ=255)                                                                 gatgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaa                                                                        >  2:107593/1‑135 (MQ=255)                                                                 gatgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaa                                                                        <  1:107593/135‑1 (MQ=255)                                                                  atgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGt                                                                  <  2:319217/140‑1 (MQ=255)                                                                  atgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGt                                                                  <  2:143237/140‑1 (MQ=255)                                                                  atgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGt                                                                  <  1:407020/140‑1 (MQ=255)                                                                   tgaAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCTGGCAGGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAAc                                                                     <  2:546675/140‑1 (MQ=255)                                                                     aaaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTaa                                                               >  1:340855/1‑140 (MQ=255)                                                                     aaaTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTaa                                                               >  1:359780/1‑140 (MQ=255)                                                                       aTTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAata                                                             <  1:252823/140‑1 (MQ=255)                                                                        tttAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAatat                                                            <  1:152411/140‑1 (MQ=255)                                                                            aaaaaTGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaa                                                                        >  1:446640/1‑124 (MQ=255)                                                                            aaaaaTGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTaa                                                                        <  2:446640/124‑1 (MQ=255)                                                                                 tGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATattatt                                                        <  2:85863/135‑1 (MQ=255)                                                                                 tGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATattatt                                                        >  1:85863/1‑135 (MQ=255)                                                                                          ccTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGc                                          >  2:46495/1‑140 (MQ=255)                                                                                               ggCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAg                                     <  2:359780/140‑1 (MQ=255)                                                                                               ggCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAg                                     <  1:591186/140‑1 (MQ=255)                                                                                               ggCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAg                                     <  2:82574/140‑1 (MQ=255)                                                                                                         gCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGa                           >  1:363026/1‑140 (MQ=255)                                                                                                           gTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACa                         <  1:538145/140‑1 (MQ=255)                                                                                                           gTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACa                         <  2:118021/140‑1 (MQ=255)                                                                                                              aTTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACt                      >  2:56021/1‑140 (MQ=255)                                                                                                                    tct‑‑‑‑GGCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCaaa                >  2:57068/1‑140 (MQ=255)                                                                                                                            gCAGGATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATaa            >  1:200360/1‑140 (MQ=255)                                                                                                                               ggATGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACg         <  2:424423/140‑1 (MQ=255)                                                                                                                                 aTGCCAGTCAAATTCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGa       <  1:56021/140‑1 (MQ=255)                                                                                                                                      aGTCAAATTCTGACGATAATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGca  >  2:201345/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                    TTACTTTCCTCTTTTGATGATCTTAAATGTCTTATTTTTCGTAATGTGTATAACAAGGAATAGTGATGAAATTTAAAAAATGTCTTCTGCCTGTGGCAATGTTAGCGTCATTCACTCT‑‑‑‑GGCAGGATGCCAGTCAAATGCTGACGATCATGCTGCCGATGTTTATCAAACCGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCA  >  NC_000913/2629730‑2629998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑