Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,122,489	G→T	I439I (ATC→ATA)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,122,489	0	G	T	100.0%	94.8 / NA	28	I439I (ATC→ATA)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/17);  total (11/17)

AGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCGATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCAATGCGGGTAAACAGTTTGTTGGCGAACGCCAC  >  NC_000913/3122353‑3122611                                                                                                                                         |                                                                                                                           aGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCtata                                                                                                                         >  1:387919/1‑140 (MQ=255)     tttGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGc                                                                                                                     <  2:269881/140‑1 (MQ=255)         aTCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACg                                                                                                                 <  1:7018/140‑1 (MQ=255)             aaGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAg                                                                                                             >  1:165020/1‑140 (MQ=255)              aGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGt                                                                                                            >  2:399613/1‑140 (MQ=255)                         tCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTc                                                                                                 <  2:165020/140‑1 (MQ=255)                          cATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTcc                                                                                                >  2:324615/1‑140 (MQ=255)                           aTCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCg                                                                                               <  2:376474/140‑1 (MQ=255)                            tCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGa                                                                                              <  1:214768/140‑1 (MQ=255)                             cTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGAc                                                                                             <  2:405462/140‑1 (MQ=255)                                 tttttACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGttc                                                                                         <  1:600366/140‑1 (MQ=255)                                   tttACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGttctt                                                                                       <  1:651388/140‑1 (MQ=255)                                     tACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCa                                                                                     <  1:405473/140‑1 (MQ=255)                                                      gCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCAtttt                                                                    >  2:65541/1‑140 (MQ=255)                                                            aTCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGt                                                              >  1:485479/1‑140 (MQ=255)                                                            aTCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGt                                                              >  2:373286/1‑140 (MQ=255)                                                               aCCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTAtt                                                           <  2:266615/140‑1 (MQ=255)                                                               aCCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTAtt                                                           <  1:324615/140‑1 (MQ=255)                                                                    aTGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTg                                                      <  2:453138/140‑1 (MQ=255)                                                                           tCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATAcc                                               <  2:485479/140‑1 (MQ=255)                                                                               cGCCGGTACGGTAGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGc                                           >  1:464287/1‑140 (MQ=255)                                                                                gCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCa                                          <  1:399613/140‑1 (MQ=255)                                                                                    gTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGc                                                     <  1:592662/125‑1 (MQ=255)                                                                                    gTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGc                                                     >  2:592662/1‑125 (MQ=255)                                                                                         gTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCaa                                 <  2:387919/140‑1 (MQ=255)                                                                                                   ccGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCAATGCGGGTaaa                       >  1:102001/1‑140 (MQ=255)                                                                                                                 cGCCACGCGGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCAATGCGGGTAAACAGTTTGTTGGCGa         >  1:103925/1‑140 (MQ=255)                                                                                                                      cgcgGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCAATGCGGGTAAACAGTTTGTTGGCGAACGcc    >  2:412196/1‑140 (MQ=255)                                                                                                                        cgGTTGCGCGCCTGAGCTATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCAATGCGGGTAAACAGTTTGTTGGCGAACGCCAc  <  1:412196/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           AGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATCACCGAATGCATTTCATCGCCGGTACGGTCGAGGAAACCGGTATTGATGAACGCCACGCGGTTGCGCGCCTGAGCGATACAGCTACGCAAGTTCAGCGAGGTCCGACGTTCTTCATCCATAATGCCCATTTTCAGGGTATTCGGTGCCATACCGAGCATTGTCTCAATGCGGGTAAACAGTTTGTTGGCGAACGCCAC  >  NC_000913/3122353‑3122611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑