Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,109	A→C	intergenic (‑368/‑115)	uxaC ← / → exuT	D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,109	0	A	C	100.0%	119.8 / NA	32	intergenic (‑368/‑115)	uxaC/exuT	D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/15);  total (17/15)

TTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCAACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGAGGTTTTACATGCGTAAAATTAAAGGGTTAC  >  NC_000913/3244973‑3245245                                                                                                                                         |                                                                                                                                         ttAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACg                                                                                                                                       >  1:163638/1‑140 (MQ=255) ttAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACg                                                                                                                                       >  2:562842/1‑140 (MQ=255)        tGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCgggg                                                                                                                                >  1:334470/1‑140 (MQ=255)                  gTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGtt                                                                                                                      <  2:68368/140‑1 (MQ=255)                    tATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGtttt                                                                                                                    >  1:274869/1‑140 (MQ=255)                      tCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGtttttt                                                                                                                  <  1:331866/140‑1 (MQ=255)                         ttCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTc                                                                                                               >  1:29451/1‑140 (MQ=255)                          tCTGCCGTCGCAAATCATAAGTCGACGGGATGCAAATTGCCGATTCATTCATTTGATAGATGAATCGGGTTAACCGGTACGGGAGCCGAATTACCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTATTTTTTCg                                                                                                              >  2:404526/1‑140 (MQ=255)                           cTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCgg                                                                                                             <  2:334470/140‑1 (MQ=255)                                gTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTAcc                                                                                                        <  2:567072/140‑1 (MQ=255)                                 tCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTAccc                                                                                                       <  2:163638/140‑1 (MQ=255)                                    cAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGt                                                                                                    <  2:9142/140‑1 (MQ=255)                                                 gACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATc                                                                                       >  1:563634/1‑140 (MQ=255)                                                 gACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATc                                                                                       >  2:152898/1‑140 (MQ=255)                                                 gACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATc                                                                                       >  1:443817/1‑140 (MQ=255)                                                       aTGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGc                                                                                 <  2:29451/140‑1 (MQ=255)                                                         gCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCct                                                                               >  1:237236/1‑140 (MQ=255)                                                               tGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCg                                                                         <  2:40946/140‑1 (MQ=255)                                                                             tttGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCcgcg                                                           <  2:176644/140‑1 (MQ=255)                                                                              ttGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCgcgc                                                          >  1:190432/1‑140 (MQ=255)                                                                                      tGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCg                                                  >  1:28850/1‑140 (MQ=255)                                                                                        aaTCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGa                                                >  1:640545/1‑140 (MQ=255)                                                                                         aTCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGat                                               >  1:376046/1‑140 (MQ=255)                                                                                          tCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGata                                              >  2:492870/1‑140 (MQ=255)                                                                                                      gTACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGTCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGAt                                  <  2:518040/140‑1 (MQ=255)                                                                                                       tACGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATg                                 >  1:298978/1‑140 (MQ=255)                                                                                                        aCGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGata                                              <  2:216432/126‑1 (MQ=255)                                                                                                        aCGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGata                                              >  1:216432/1‑126 (MQ=255)                                                                                                        aCGGAAGCCGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGa                                <  2:274869/140‑1 (MQ=255)                                                                                                                cGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGAGGTTTTAc                        >  2:36383/1‑140 (MQ=255)                                                                                                                cGAATTAGCACGAAACTTTCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGAGGTTTTAc                        <  2:190432/140‑1 (MQ=255)                                                                                                                                tttCATGGCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGAGGTTTTACATGCGTAAAATTAAAg        <  2:376046/140‑1 (MQ=255)                                                                                                                                      ggCCACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGAGGTTTTACATGCGTAAAATTAAAGGGTTAc  <  1:492870/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAACCGGTACGGAAGCCGAATTAGCACGAAACTTTCATGGCAACGTTCGGGGCGTGCCGGTTTTTTTTCGGTTACCCGGTCGTAACTAACATCTTCAGCCTCTGGCGGGATGATGGCCGCGCTTCCTGCGGATATAACAAAACGATGAGGTTTTACATGCGTAAAATTAAAGGGTTAC  >  NC_000913/3244973‑3245245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑