Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,997,400	C→A	T73K (ACG→AAG)	yigB →	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,997,400	0	C	A	100.0%	63.2 / NA	19	T73K (ACG→AAG)	yigB	5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/8);  total (11/8)

AGCGAGAGGCGCTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAAC  >  NC_000913/3997277‑3997535                                                                                                                            |                                                                                                                                        agcgagAGGCGCTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTttcgt                                                                                                                         >  1:143683/1‑140 (MQ=255)          cgcTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGaa                                                                                                                <  2:564007/140‑1 (MQ=255)                 cTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGa                                                                                                         <  2:566066/140‑1 (MQ=255)                     gtgCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCt                                                                                                     <  2:290701/140‑1 (MQ=255)                                     cGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGt                                                                                     >  2:374334/1‑140 (MQ=255)                                       gcgcTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCggg                                                                                           <  1:447090/132‑1 (MQ=255)                                       gcgcTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCggg                                                                                           >  2:447090/1‑132 (MQ=255)                                                aGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGc                                                                          <  2:586898/140‑1 (MQ=255)                                                                    gCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACgcag                                                      <  1:613891/140‑1 (MQ=255)                                                                          ccTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGa                                                <  2:143683/140‑1 (MQ=255)                                                                                   ggCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCATTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTg                                       >  2:454108/1‑140 (MQ=255)                                                                                        tACGGGAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCaaa                                  >  1:562590/1‑140 (MQ=255)                                                                                             gAAGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGGGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGgcg                             >  2:36510/1‑140 (MQ=255)                                                                                               aGCGGAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCa                           >  1:18694/1‑140 (MQ=255)                                                                                                  ggAACCCGAGATTTATCACGACGTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGcc                        >  2:418146/1‑140 (MQ=255)                                                                                                                    cgacgTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCgcagc      <  1:490055/140‑1 (MQ=255)                                                                                                                     gacgTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCgcagca     >  2:513021/1‑140 (MQ=255)                                                                                                                     gacgTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCgcagca     >  2:375795/1‑140 (MQ=255)                                                                                                                      acgTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCaa    >  2:90074/1‑140 (MQ=255)                                                                                                                        gTGAAGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAAc  >  1:400644/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                        AGCGAGAGGCGCTTACCTTTGTGCAAAATTATCATCCGGCGCTGCGCAGCTTCCAGAATGAAGATCTGCAACGCCTGCGCCAGGCGGTACGGGAAGCGGAACCCGAGATTTATCACGACGTGACGCGCTGGCGTTTTCGTTCGATTGAACAAGCGATGCTCGACGCCGGGCTGAGTGCCGAAGAAGCCAGTGCAGGCGCACACGCAGCAATGATCAACTTTGCCAAATGGCGCAGCCGAATCGACGTCCCGCAGCAAAC  >  NC_000913/3997277‑3997535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑