Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,112,586	C→A	A91E (GCG→GAG)	yiiR →	DUF805 domain‑containing protein YiiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,112,586	0	C	A	100.0%	41.0 / NA	13	A91E (GCG→GAG)	yiiR	DUF805 domain‑containing protein YiiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (1/12);  total (1/12)

GACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATG  >  NC_000913/4112449‑4112720                                                                                                                                           |                                                                                                                                       gACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAAT‑GGgaga                                                                                                                                      >  1:610512/1‑140 (MQ=255)                            gctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAAT‑GGGGGGTTTTTCCGGGGGTCTGGCAATgggggg                                                                                                           >  1:375166/1‑140 (MQ=255)                                           ggCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGCGGGGCGTTTTGTcc                                                                                            <  2:502313/140‑1 (MQ=255)                                            gCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTccc                                                                                           <  1:276469/140‑1 (MQ=255)                                               gTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAATCGGGGGTTTTTCCGGGGGGTTGGGAAAGGGGGGGGGGGGGGTTTTTTCCCgc                                                                                         >  1:103424/1‑139 (MQ=255)                                                tAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACg                                                                                       <  1:55712/140‑1 (MQ=255)                                                          gcgcTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAAT‑GGGGGGTTTTTCCCGGGGGGTGGGAAAGGGGGGGGGGGGGGTTTTTCCCCGCGTTGGtttttg                                                                             >  2:55712/1‑136 (MQ=255)                                                                     aTCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCtt                                                                  <  1:354398/140‑1 (MQ=255)                                                                                tCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAAT‑GGGGGATTTTTACGGGGGGCTGGCGATGGGGGGGGGGGGGGTTTGTCCCCGCGGTGGTTTTTGGGATGATGGTTTTCGATCtttg                                                       >  2:333390/1‑138 (MQ=255)                                                                                 ccGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGt                                                      <  1:431153/140‑1 (MQ=255)                                                                                                      tgatTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGc                                 <  1:410160/140‑1 (MQ=255)                                                                                                             ggCGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAaaata                          <  1:29583/140‑1 (MQ=255)                                                                                                               cGTGGATGCTGCTGGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAaaataaa                        <  1:625646/140‑1 (MQ=255)                                                                                                                 tGGATGCTGCTGGCGGGGAAAT‑GGGGGAATTTATCCGGGGTTTGGCAAAGGGGGGGGGGGGGCTTTGTTCCCAAGTTGATTTTGGGGATTATGCTTATCGATTTTGTTGCGTTTTTTGGCACACAAGGCGAaaataaaaa                      >  2:625646/1‑138 (MQ=255)                                                                                                                          ctgGCGGGTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATGGTAAAGa             <  1:54892/140‑1 (MQ=255)                                                                                                                                ggTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATgg                   <  2:452658/128‑1 (MQ=255)                                                                                                                                ggTAACT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATgg                   >  1:452658/1‑128 (MQ=255)                                                                                                                                    aCT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGAt   <  1:413868/140‑1 (MQ=255)                                                                                                                                     cT‑GGGAGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGAtg  <  1:30701/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       GACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTTATCGATCTTGGTGCGTTTGTTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATG  >  NC_000913/4112449‑4112720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑