Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,340,044	G→T	P160T (CCA→ACA)	adiA ←	arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,044	0	G	T	100.0%	101.5 / NA	30	P160T (CCA→ACA)	adiA	arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/18);  total (12/18)

CCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTAT  >  NC_000913/4339908‑4340169                                                                                                                                         |                                                                                                                              ccAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGtcgc                                                                                                                            <  2:114443/140‑1 (MQ=255) ccAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGtcgc                                                                                                                            >  1:60835/1‑140 (MQ=255)   aGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTg                                                                                                                          <  2:356515/140‑1 (MQ=255)   aGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTg                                                                                                                          <  2:231869/140‑1 (MQ=255)        ggTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAg                                                                                                                     <  2:9289/140‑1 (MQ=255)        ggTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAg                                                                                                                     <  1:557177/140‑1 (MQ=255)              gCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAatat                                                                                                               >  2:443236/1‑140 (MQ=255)               cAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATAtt                                                                                                              >  1:148070/1‑140 (MQ=255)                 aaGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCa                                                                                                            <  1:443236/140‑1 (MQ=255)                      ccGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGACCTGTCGCTGCCCAGGAATATTCATGGAt                                                                                                       >  1:437408/1‑140 (MQ=255)                        gAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGt                                                                                                     <  2:339587/140‑1 (MQ=255)                           ggAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCAc                                                                                                  <  1:266174/140‑1 (MQ=255)                           ggAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCAc                                                                                                  <  1:538850/140‑1 (MQ=255)                                     tcgATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcat                                                                                        >  1:87969/1‑140 (MQ=255)                                        aTGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAg                                                                                     >  1:65086/1‑140 (MQ=255)                                            ccATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcg                                                                                 <  1:510041/140‑1 (MQ=255)                                                     tGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGt                                                                        >  2:70923/1‑140 (MQ=255)                                                     tGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGt                                                                        <  1:434673/140‑1 (MQ=255)                                                     tGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGt                                                                        >  2:434673/1‑140 (MQ=255)                                                          aaCAGATTTTCAACATAGTAGTCATGGAAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGCGGCTTGTCGCTGCCCAGGAATATTCCTGGATGTCACTTTATTCCATCAGCGCGCTGAACAGcggcgg                                                                   >  2:473670/1‑140 (MQ=255)                                                              gATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAAc                                                               <  1:377200/140‑1 (MQ=255)                                                                   tCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAgctg                                                          <  2:437408/140‑1 (MQ=255)                                                                      ccATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGctgctg                                                       <  2:65086/140‑1 (MQ=255)                                                                          agtagtCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCgg                                                   >  2:267969/1‑140 (MQ=255)                                                                                    gTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGTGGCAACAGCTGCTGGCGGTAGCGGGTCa                                         >  2:74476/1‑140 (MQ=255)                                                                                    gTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGgggggcg                                         >  2:391997/1‑137 (MQ=255)                                                                                      aGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGGGGGTCATc                                       >  1:496366/1‑140 (MQ=255)                                                                                      aGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGGAGCGGGGCATc                                       >  2:600642/1‑140 (MQ=255)                                                                                       gAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATcg                                      <  1:70923/140‑1 (MQ=255)                                                                                       gAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATcg                                      <  2:87969/140‑1 (MQ=255)                                                                                                             gTAAAACCAACGCCGCCCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGAt                <  2:60835/140‑1 (MQ=255)                                                                                                                         ccgccCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGt    <  1:267969/140‑1 (MQ=255)                                                                                                                          cgccCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTa   <  2:496366/140‑1 (MQ=255)                                                                                                                           gccCTGGTGGCCTGTCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCCAGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTAt  <  1:473670/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                              CCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTAT  >  NC_000913/4339908‑4340169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑