Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	927,511	C→A	R562I (AGA→ATA)	cydC ←	glutathione/L‑cysteine ABC exporter subunit CydC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	927,511	0	C	A	100.0%	163.8 / NA	46	R562I (AGA→ATA)	cydC	glutathione/L‑cysteine ABC exporter subunit CydC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/25);  total (21/25)

TGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTGCAAG  >  NC_000913/927376‑927647                                                                                                                                        |                                                                                                                                         tGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGc                                                                                                                                      >  1:562338/1‑140 (MQ=255)    gcCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAg                                                                                                                                   <  2:562338/140‑1 (MQ=255)      cTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCa                                                                                                                                 >  1:337418/1‑140 (MQ=255)       ttCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAg                                                                                                                                >  1:201601/1‑140 (MQ=255)            gggaagggaaGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTg                                                                                                                           <  2:393064/140‑1 (MQ=255)            gggaagggaaGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTg                                                                                                                           <  2:337418/140‑1 (MQ=255)            gggaagggaaGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTg                                                                                                                           <  2:222072/140‑1 (MQ=255)                       gCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCt                                                                                                                <  2:453710/140‑1 (MQ=255)                       gCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCt                                                                                                                <  2:177495/140‑1 (MQ=255)                        cTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCtt                                                                                                               <  2:328433/140‑1 (MQ=255)                           ttGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCt                                                                                                            >  1:446179/1‑140 (MQ=255)                                   cGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAAtt                                                                                                    >  1:141216/1‑140 (MQ=255)                                   cGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAAtt                                                                                                    >  2:482165/1‑140 (MQ=255)                                    gagaAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAAttt                                                                                                   <  2:141216/140‑1 (MQ=255)                                       aaaGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGcc                                                                                                <  2:11779/140‑1 (MQ=255)                                         aGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCg                                                                                              <  2:626328/140‑1 (MQ=255)                                            tGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTg                                                                                           >  1:562267/1‑140 (MQ=255)                                                 cAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCAt                                                                                      <  2:201601/140‑1 (MQ=255)                                                   ggCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCa                                                                                    >  1:547233/1‑140 (MQ=255)                                                     cgcATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACt                                                                                  <  2:580376/140‑1 (MQ=255)                                                             cGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATttg                                                                          <  2:446179/140‑1 (MQ=255)                                                                 aaaCTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTgg                                                                      <  2:118670/140‑1 (MQ=255)                                                                   aCTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGaa                                                                    <  2:198334/140‑1 (MQ=255)                                                                       cACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACga                                                                >  2:313157/1‑140 (MQ=255)                                                                              gTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTc                                                         >  2:383708/1‑140 (MQ=255)                                                                                  gATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCcgcg                                                     <  1:313157/140‑1 (MQ=255)                                                                                  gATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCcgcg                                                     <  2:361515/140‑1 (MQ=255)                                                                                        tCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCg                                               >  2:505816/1‑140 (MQ=255)                                                                                             aataGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATggg                                          <  1:482165/140‑1 (MQ=255)                                                                                                aGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCcgcg                                                     >  2:352613/1‑126 (MQ=255)                                                                                                aGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCcgcg                                                     <  1:352613/126‑1 (MQ=255)                                                                                                  cTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGAcc                                     >  1:57248/1‑140 (MQ=255)                                                                                                  cTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGAcc                                     >  1:387788/1‑140 (MQ=255)                                                                                                    tACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCcgcg                                                     <  2:333248/122‑1 (MQ=255)                                                                                                    tACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCcgcg                                                     >  1:333248/1‑122 (MQ=255)                                                                                                    tACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTg                                        >  2:202265/1‑135 (MQ=255)                                                                                                    tACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTg                                        <  1:202265/135‑1 (MQ=255)                                                                                                                    aCTGGTAATAACGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCa                   <  2:57248/140‑1 (MQ=255)                                                                                                                               cGCCCCTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTc        >  1:506477/1‑140 (MQ=255)                                                                                                                                  cccTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgc     >  1:366574/1‑140 (MQ=255)                                                                                                                                  cccTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgc     >  2:42300/1‑140 (MQ=255)                                                                                                                                  cccTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgc     >  2:213323/1‑140 (MQ=255)                                                                                                                                  cccTGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgc     >  2:178750/1‑140 (MQ=255)                                                                                                                                     tGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgcaag  <  1:42300/140‑1 (MQ=255)                                                                                                                                     tGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgcaag  <  2:506477/140‑1 (MQ=255)                                                                                                                                     tGTATGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTgcaag  <  2:562267/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCGCCTTCCGGGGAAGGGAAGGCTATTGAATGGCGAGAAAGCTGAACCAGGCGCATGACCGCAAAACTCCACGCAAGTCGGATCGTTCAATAATAGCTTACAAACCCTGCTTGAACTGGTAATAACGCCCCTGTCTGGCAAGCAGTTCTGCGTGAGTACCTTGCTCAATAATTTGCCCGTTGTCCATCACTATTATTTGTTGGAAACGAGAGAGTCCGCGAAGTCGATGGGTGACCATTAACACCGTTTTCTCACGCATCATTTCTGCAAG  >  NC_000913/927376‑927647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑