Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	224,699	(G)5→4	noncoding (929/1542 nt)	rrsH →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	224,695	0	G	.	100.0%	54.1 / NA	15	noncoding (925/1542 nt)	rrsH	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/6);  total (9/6)

CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATG  >  NC_000913/224632‑224827                                                                |                                                                                                                                     cTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCcaca                                                         <  1:346413/140‑1 (MQ=11)     cgcgTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAAc                                                     <  2:319539/140‑1 (MQ=18)              tCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCagag                                            <  2:302609/140‑1 (MQ=255)                      cTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTg                                    >  1:285523/1‑140 (MQ=255)                        ggggAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGt                                  <  2:156462/140‑1 (MQ=255)                              tACGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTc                            >  2:413754/1‑140 (MQ=255)                                cGGCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCgg                          >  2:297743/1‑140 (MQ=255)                                 ggCCGCAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCggg                         <  2:291973/140‑1 (MQ=255)                                      cAAGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACtg                    >  2:23241/1‑140 (MQ=255)                                        aGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACtgtg                  >  2:102355/1‑140 (MQ=255)                                        aGGTTAAAACTCAAATGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGCGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGCCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACtgtg                  >  2:136260/1‑140 (MQ=255)                                                    aaaTGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGtgctg      >  1:493215/1‑140 (MQ=255)                                                     aaTGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTga                 <  1:122629/128‑1 (MQ=255)                                                     aaTGAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTga                 >  2:122629/1‑128 (MQ=255)                                                        gAATTGAC‑GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATg  >  1:260685/1‑140 (MQ=255)                                                                |                                                                                                                                     CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATG  >  NC_000913/224632‑224827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑