Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,674	T→C	intergenic (‑148/+47)	fnr ← / ← ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,674	0	T	C	100.0%	123.6 / NA	33	intergenic (‑148/+47)	fnr/ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

AAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCAAT  >  NC_000913/1399537‑1399810                                                                                                                                          |                                                                                                                                         aaGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCaa                                                                                                                                        <  2:475077/140‑1 (MQ=255)   gCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACt                                                                                                                                      >  2:330958/1‑140 (MQ=255)               atTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAgt                                                                                                                          <  2:117462/140‑1 (MQ=255)                       aTTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACaa                                                                                                                  >  2:381441/1‑140 (MQ=255)                                             cAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGt                                                                                            >  2:174512/1‑140 (MQ=255)                                               agtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGttt                                                                                          <  1:190784/140‑1 (MQ=255)                                                                      agcagAGAATTCCGATATTAGATGTAAATATATGTCGATGTATTTGAAATCCCTTAAGGTGTTAAGGGCAACTGTACATAAAAGTGTGAACAAGCTGGCACAAATGGTTTAATGTTTACAGCAAAAGATAACCTTCATgg                                                                   <  2:431439/140‑1 (MQ=255)                                                                        cagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATg                                                                    <  2:438272/137‑1 (MQ=255)                                                                        cagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATg                                                                    >  1:438272/1‑137 (MQ=255)                                                                        cagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcg                                                                 <  1:174512/140‑1 (MQ=255)                                                                        cagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgcg                                                                 >  1:220251/1‑140 (MQ=255)                                                                            gaATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAAt                                                             <  2:300135/140‑1 (MQ=255)                                                                                          gATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCg                                               <  2:254837/140‑1 (MQ=255)                                                                                           aTGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGc                                              <  2:485666/140‑1 (MQ=255)                                                                                            tGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCt                                             >  1:499339/1‑140 (MQ=255)                                                                                                aaTATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAAc                                         >  1:24163/1‑140 (MQ=255)                                                                                                      tGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGcc                                   <  1:330958/140‑1 (MQ=255)                                                                                                       gTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCttt                                                  >  1:75574/1‑124 (MQ=255)                                                                                                       gTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCttt                                                  <  2:75574/124‑1 (MQ=255)                                                                                                        tctatctaTTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTg                                 >  2:118697/1‑140 (MQ=255)                                                                                                          tatctatTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCa                               >  1:80821/1‑140 (MQ=255)                                                                                                          tatctatTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCa                               >  1:451365/1‑140 (MQ=255)                                                                                                            tctatTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCTAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                             >  2:109992/1‑140 (MQ=255)                                                                                                            tctatTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                             >  2:265886/1‑140 (MQ=255)                                                                                                            tctatTTGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                             >  1:171774/1‑140 (MQ=255)                                                                                                                 ttGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCgg                        <  1:265886/140‑1 (MQ=255)                                                                                                                  tGAAAACCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGt                       >  1:217088/1‑140 (MQ=255)                                                                                                                    aaaaCCCTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCa                     <  1:118697/140‑1 (MQ=255)                                                                                                                         ccTTAAGTTGTTAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTg                <  2:80821/140‑1 (MQ=255)                                                                                                                                   ttAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCa                     >  2:447181/1‑125 (MQ=255)                                                                                                                                   ttAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCa                     <  1:447181/125‑1 (MQ=255)                                                                                                                                   ttAAGGGCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGc      >  1:400256/1‑140 (MQ=255)                                                                                                                                       gggCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCAAt  <  2:400256/140‑1 (MQ=255)                                                                                                                                       gggCAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTCCATGGCGCAATAACCGCTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCAAt  <  1:109992/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCAAT  >  NC_000913/1399537‑1399810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑