Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,604,033	C→A	R339R (CGG→AGG)	lsrC →	Autoinducer‑2 ABC transporter membrane subunit LsrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,033	0	C	A	100.0%	108.4 / NA	31	R339R (CGG→AGG)	lsrC	Autoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/20);  total (11/20)

GGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCAC  >  NC_000913/1603897‑1604162                                                                                                                                         |                                                                                                                                  gggTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAggg                                                                                                                                <  1:122440/140‑1 (MQ=255)         ttCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCa                                                                                                                        <  2:431142/140‑1 (MQ=255)         ttCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCa                                                                                                                        <  1:305503/140‑1 (MQ=255)         ttCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCa                                                                                                                        <  1:291435/140‑1 (MQ=255)                 gCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGt                                                                                                                <  2:331113/140‑1 (MQ=255)                 gCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGt                                                                                                                <  2:121647/140‑1 (MQ=255)                  cGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTa                                                                                                               <  1:261366/140‑1 (MQ=255)                         ggTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTa                                                                                                        <  1:160680/140‑1 (MQ=255)                                            cGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCtt                                                                                     >  1:173026/1‑140 (MQ=255)                                             gTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTg                                                                                    <  1:70732/140‑1 (MQ=255)                                                 tgCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGc                                                                                >  1:223794/1‑140 (MQ=255)                                                 tgCGCTGGAACGTAATCTACGGCGGC‑AAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCa                                                                               <  2:59954/140‑1 (MQ=255)                                                             tAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTAt                                                                    >  2:224673/1‑140 (MQ=255)                                                                      gcggcAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGt                                                           >  2:141915/1‑140 (MQ=255)                                                                       cggcAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGc                                                                           <  1:28408/123‑1 (MQ=255)                                                                       cggcAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGc                                                                           >  2:28408/1‑123 (MQ=255)                                                                       cggcAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTc                                                          >  2:425154/1‑140 (MQ=255)                                                                          cAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCa                                                       >  1:239623/1‑140 (MQ=255)                                                                          cAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCa                                                       <  2:173026/140‑1 (MQ=255)                                                                           aaaaaTATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCAt                                                      >  2:63845/1‑140 (MQ=255)                                                                               atatGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTgg                                                  <  2:239623/140‑1 (MQ=255)                                                                                   gCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCa                                              >  2:321269/1‑140 (MQ=255)                                                                                                   cACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGt                              >  2:310517/1‑140 (MQ=255)                                                                                                    aCCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGtt                             <  1:224673/140‑1 (MQ=255)                                                                                                     ccGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTa                            <  1:321269/140‑1 (MQ=255)                                                                                                      cGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAg                           <  2:223794/140‑1 (MQ=255)                                                                                                      cGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTAGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAg                           <  1:325486/140‑1 (MQ=255)                                                                                                               ttAAACCCGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAata                  <  1:63845/140‑1 (MQ=255)                                                                                                                    cccGCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTg             <  1:425154/140‑1 (MQ=255)                                                                                                                       gCTTCGTCAGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTg          >  2:73316/1‑140 (MQ=255)                                                                                                                               aGGTAAAAAAAGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCAc  <  1:73316/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCCGCACTGCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCAC  >  NC_000913/1603897‑1604162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑