BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,336	C→A	R73S (CGT→AGT)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,336	0	C	A	100.0%	127.0 / NA	36	R73S (CGT→AGT)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/18); total (18/18)

TAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCC  >  NC_000913/1619206‑1619468

tAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTgg                                                                                                                             <  2:228829/140‑1 (MQ=255)

tAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTgg                                                                                                                             <  2:168341/140‑1 (MQ=255)

 aaCGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGa                                                                                                                            >  1:204268/1‑140 (MQ=255)

        aTCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTg                                                                                                                     >  1:45539/1‑140 (MQ=255)

         tCTGTCTCCGCTGGATATTGCCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTgg                                                                                                                    <  2:153300/140‑1 (MQ=255)

         tCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTgg                                                                                                                    <  2:378279/140‑1 (MQ=255)

         tCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTgg                                                                                                                    >  2:215064/1‑140 (MQ=255)

                             ccGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGaa                                                                                                >  1:398526/1‑140 (MQ=255)

                                                 ctctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGa                                                                            >  1:243880/1‑140 (MQ=255)

                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGcc                                                                                        >  1:410109/1‑127 (MQ=255)

                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGcc                                                                                        <  2:410109/127‑1 (MQ=255)

                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGAc                                                                           >  1:454397/1‑140 (MQ=255)

                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGAc                                                                           >  2:40162/1‑140 (MQ=255)

                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGAc                                                                           >  1:254415/1‑140 (MQ=255)

                                                              gCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa                                                               <  1:304757/140‑1 (MQ=255)

                                                                tGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACt                                                             >  1:269121/1‑140 (MQ=255)

                                                                                 tCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcg                                            >  1:273459/1‑140 (MQ=255)

                                                                                     gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTTGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                        >  1:400101/1‑140 (MQ=255)

                                                                                     gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                        >  1:424304/1‑140 (MQ=255)

                                                                                     gTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcgg                                        >  1:451373/1‑140 (MQ=255)

                                                                                      ttGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCggcggc                                       <  2:424304/140‑1 (MQ=255)

                                                                                           cTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                                  <  2:243880/140‑1 (MQ=255)

                                                                                           cTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                                  <  2:398526/140‑1 (MQ=255)

                                                                                           cTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                                  <  1:215064/140‑1 (MQ=255)

                                                                                            tGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCa                                 <  1:386425/140‑1 (MQ=255)

                                                                                                aaaGGTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAatat                             <  2:454397/140‑1 (MQ=255)

                                                                                                   ggTATTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtgtg                          <  1:64701/140‑1 (MQ=255)

                                                                                                      aTTGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAAc                       <  2:195580/140‑1 (MQ=255)

                                                                                                       ttGTCGGTCGACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACa                      >  2:117387/1‑140 (MQ=255)

                                                                                                          tcggtcgACCTGGGAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATg                   <  2:204268/140‑1 (MQ=255)

                                                                                                                     gggAGCACTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAg        <  2:451373/140‑1 (MQ=255)

                                                                                                                           aCTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc  >  2:316659/1‑140 (MQ=255)

                                                                                                                            cTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGcc                 <  2:289851/124‑1 (MQ=255)

                                                                                                                            cTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGcc                 >  1:289851/1‑124 (MQ=255)

                                                                                                                            cTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc  >  2:300358/1‑139 (MQ=255)

                                                                                                                            cTGACCAGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc  <  1:300358/139‑1 (MQ=255)

TAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCC  >  NC_000913/1619206‑1619468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`