Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,494,132	T→C	T91A (ACC→GCC)	yfdX ←	protein YfdX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,494,132	0	T	C	100.0%	176.8 / NA	46	T91A (ACC→GCC)	yfdX	protein YfdX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

GGCAATTTTTATCGCGGCTTCTTTTTCGGGAGTTGCTACATAACTCTCAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTG  >  NC_000913/2493995‑2494266                                                                                                                                          |                                                                                                                                       ggCAATTTTTATCGCGGCTTCTTTTTCGGGAGTTGCTACATAACTCTCAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCAc                                                                                                                                      <  2:397101/140‑1 (MQ=255)                              gAGTTGCTACATAACTCTCAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCa                                                                                                         <  1:225658/140‑1 (MQ=255)                                            ctctCAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCttt                                                                                           >  1:199654/1‑140 (MQ=255)                                              ctcAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTg                                                                                         >  1:323778/1‑140 (MQ=255)                                               tcAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGc                                                                                        <  1:277738/140‑1 (MQ=255)                                               tcAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGc                                                                                        >  1:70961/1‑140 (MQ=255)                                                 aGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCt                                                                                      >  2:347104/1‑140 (MQ=255)                                                 aGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCt                                                                                      >  2:228347/1‑140 (MQ=255)                                                 aGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCt                                                                                      >  1:427759/1‑140 (MQ=255)                                                      ttCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCt                                                                                 >  2:223276/1‑140 (MQ=255)                                                          gACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGAt                                                                             <  2:151904/140‑1 (MQ=255)                                                           aCAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGAt                                                                             >  1:85222/1‑139 (MQ=255)                                                           aCAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGAt                                                                             <  2:85222/139‑1 (MQ=255)                                                            cAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCg                                                                           <  1:443208/140‑1 (MQ=255)                                                                             atatatTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGaaa                                                                   >  1:497021/1‑131 (MQ=255)                                                                             atatatTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGaaa                                                                   <  2:497021/131‑1 (MQ=255)                                                                              tatatTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAgg                                                         <  2:70961/140‑1 (MQ=255)                                                                               atatTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGgc                                                        >  2:60878/1‑140 (MQ=255)                                                                                 atTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGgcgc                                                      >  2:228200/1‑140 (MQ=255)                                                                                 atTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGgcgc                                                      <  1:347104/140‑1 (MQ=255)                                                                                 atTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGgcgc                                                      >  1:47825/1‑140 (MQ=255)                                                                                      tcatcaTTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTAc                                                 >  2:347526/1‑140 (MQ=255)                                                                                      tcatcaTTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTAc                                                 >  1:166865/1‑140 (MQ=255)                                                                                      tcatcaTTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTAc                                                 <  2:187751/140‑1 (MQ=255)                                                                                      tcatcaTTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTAc                                                 >  1:267915/1‑140 (MQ=255)                                                                                        atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCt                                               >  1:103712/1‑140 (MQ=255)                                                                                        atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCt                                               >  1:310172/1‑140 (MQ=255)                                                                                        atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCt                                               >  1:411147/1‑140 (MQ=255)                                                                                        atcatTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAAAAGAGCCAGGCGCGCTACCt                                               >  2:225372/1‑140 (MQ=255)                                                                                          catTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGc                                             >  1:84041/1‑140 (MQ=255)                                                                                          catTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGc                                             >  2:193954/1‑140 (MQ=255)                                                                                                     gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCAcgcat                                  <  1:223276/140‑1 (MQ=255)                                                                                                     gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCAcgcat                                  <  2:267915/140‑1 (MQ=255)                                                                                                     gTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCAcgcat                                  <  2:301867/140‑1 (MQ=255)                                                                                                      tCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCAcgcatc                                 <  2:199654/140‑1 (MQ=255)                                                                                                                tGGCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAAc                       >  2:340420/1‑140 (MQ=255)                                                                                                                 ggCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAAcc                      <  1:400276/140‑1 (MQ=255)                                                                                                                  gCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAGGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCAcgcat                                  >  2:410603/1‑127 (MQ=255)                                                                                                                  gCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCAcgcat                                  <  1:410603/127‑1 (MQ=255)                                                                                                                  gCTTAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCt                     >  2:4005/1‑140 (MQ=255)                                                                                                                    ttAGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTg                   <  1:60878/140‑1 (MQ=255)                                                                                                                      aGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGtt                 <  1:193954/140‑1 (MQ=255)                                                                                                                      aGCGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGtt                 >  2:117344/1‑140 (MQ=255)                                                                                                                        cGAACTTTGCCCATTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCa               >  2:381583/1‑140 (MQ=255)                                                                                                                                    aTTCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGctct   <  1:228347/140‑1 (MQ=255)                                                                                                                                     ttCGGCACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTctg  <  1:117344/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GGCAATTTTTATCGCGGCTTCTTTTTCGGGAGTTGCTACATAACTCTCAGAAATTCCGACAGATGCATTAATGACAATATATTGGTCATCATTGAGATTGGTCTTTTTACCTGGCTTAGCGAACTTTGCCCATTCGGTACTGTCATCTGACAGCAAAGCGGAAGCTTCATTGGTCAGTTCTTTTGCCTTTTCTGGATCGCCATGAAACAGAGCCAGGCGCGCTACCTGCACGTCACGCATCGCATATAAACCTTGTTCAGAGATACGCTCTG  >  NC_000913/2493995‑2494266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑