Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,749,752	G→T	G327C (GGC→TGC)	yfjD →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,749,752	0	G	T	100.0%	82.5 / NA	24	G327C (GGC→TGC)	yfjD	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/9);  total (15/9)

TGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATC  >  NC_000913/2749618‑2749875                                                                                                                                       |                                                                                                                            tgCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGAt                                                                                                                        <  1:295853/140‑1 (MQ=255)                       cACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCaa                                                                                                  >  1:440626/1‑140 (MQ=255)                             gTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTg                                                                                            <  2:180669/140‑1 (MQ=255)                                ggTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCg                                                                                         >  1:481846/1‑140 (MQ=255)                                 gTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGa                                                                                        >  2:62971/1‑140 (MQ=255)                                        ttCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTc                                                                                 >  2:112585/1‑140 (MQ=255)                                           aGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCAcg                                                                              >  1:183276/1‑140 (MQ=255)                                                                 ccTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGa                                                        >  2:31536/1‑140 (MQ=255)                                                                   tGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGAtt                                                      <  2:285362/140‑1 (MQ=255)                                                                     gtcgtcAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTAt                                                    >  2:393719/1‑140 (MQ=255)                                                                                          aTTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCg                               >  1:377796/1‑140 (MQ=255)                                                                                           ttCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCgg                              >  2:123148/1‑140 (MQ=255)                                                                                                gggCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAAt                         >  2:58817/1‑140 (MQ=255)                                                                                                     ggTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATcaaca                    >  2:45941/1‑140 (MQ=255)                                                                                                     ggTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATcaaca                    <  2:440626/140‑1 (MQ=255)                                                                                                     ggTGACGGTTGAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATcaaca                    >  2:160797/1‑140 (MQ=255)                                                                                                               gAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTaa          <  1:123148/140‑1 (MQ=255)                                                                                                               gAAGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTaa          <  1:45941/140‑1 (MQ=255)                                                                                                                aaGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATcaaca                    >  2:229161/1‑129 (MQ=255)                                                                                                                aaGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATcaaca                    <  1:229161/129‑1 (MQ=255)                                                                                                                 aGATATTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACt        >  1:410615/1‑140 (MQ=255)                                                                                                                   atatTCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTgg      <  1:31536/140‑1 (MQ=255)                                                                                                                      ttCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGTGAAATCAACAAAGCCTTTAACTGGCAt   <  1:100110/140‑1 (MQ=255)                                                                                                                       tCTGGAAGAGATTGTCTGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATc  >  1:411787/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                            TGCCGGAAGGTACGCCGCTCAGCACGCAGTTGGTAAAGTTTCAGCGCAACAAAAAGAAAGTCGGCCTGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATC  >  NC_000913/2749618‑2749875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑