Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,495	G→T	G744G (GGC→GGA)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,495	0	G	T	100.0%	97.0 / NA	29	G744G (GGC→GGA)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/16);  total (13/16)

GTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTT  >  NC_000913/2780361‑2780631                                                                                                                                       |                                                                                                                                         gtgtCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTg                                                                                                                                     >  1:274541/1‑140 (MQ=255)       gACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTAct                                                                                                                               <  2:364819/140‑1 (MQ=255)       gACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTAct                                                                                                                               <  1:497682/140‑1 (MQ=255)         cTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTActct                                                                                                                             <  2:73085/140‑1 (MQ=255)         cTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTActct                                                                                                                             >  1:235496/1‑140 (MQ=255)          tCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTctg                                                                                                                            <  1:300813/140‑1 (MQ=255)            cACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTctgct                                                                                                                          <  1:296766/140‑1 (MQ=255)            cACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTctgct                                                                                                                          >  1:338499/1‑140 (MQ=255)              cGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGt                                                                                                                        >  1:110972/1‑140 (MQ=255)              cGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTCCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGt                                                                                                                        >  1:63997/1‑140 (MQ=255)                           gccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTgcgc                                                                                                           <  1:227766/140‑1 (MQ=255)                           gccATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTgcgc                                                                                                           >  2:227766/1‑140 (MQ=255)                             cATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTg                                                                                                         <  2:119222/140‑1 (MQ=255)                               tttGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGcc                                                                                                       >  1:105208/1‑140 (MQ=255)                                                                         aaCGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCAcc                                                               >  2:101493/1‑138 (MQ=255)                                                                         aaCGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCAcc                                                               <  1:101493/138‑1 (MQ=255)                                                                                      ccGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAgg                                                <  2:346538/140‑1 (MQ=255)                                                                                         cgcgTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGgccg                                             <  2:105208/140‑1 (MQ=255)                                                                                             tgtTCCTGTGACCCGCGTCGCCCTGGTGGTCGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCt                                         <  2:488774/140‑1 (MQ=255)                                                                                                         ccGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCt                             <  1:317566/140‑1 (MQ=255)                                                                                                         ccGCGTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCt                             >  2:460575/1‑140 (MQ=255)                                                                                                            cgTCGCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCtgtg                          >  2:164516/1‑140 (MQ=255)                                                                                                                gCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCTGTGGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAAcc                      <  2:63997/140‑1 (MQ=255)                                                                                                                gCCCTGGTGGTTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCTCCGAGTTTTCAGCCAGGCCGCCCTCATTACCTACCTGTGAAcc                      <  2:110972/140‑1 (MQ=255)                                                                                                                       tggtTGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAGCCCCCATta               <  2:235496/140‑1 (MQ=255)                                                                                                                           tGCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATtaataa           >  2:415568/1‑140 (MQ=255)                                                                                                                            gCAACCAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTaataat          >  1:73183/1‑140 (MQ=255)                                                                                                                                ccAAAGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGaa      >  1:154676/1‑140 (MQ=255)                                                                                                                                  aaaGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAAcc    <  1:460575/140‑1 (MQ=255)                                                                                                                                    aGCTCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCtt  <  2:274541/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGCCCTGGTGGTTGCAACCAAAGCGCCCTGGCTACTCTGCTGTATCCCCGTTGCGCTGCCTTTCTCCCGCACATCGAGTGTGCCGCCGTCATTAAGCACCGAGTTTTCAGCCAGGCCGCCCTCATTAACTACCTGTGAACCCCCATTAATAATGGAACCTT  >  NC_000913/2780361‑2780631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑