Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	319,759	C→A	K81N (AAG→AAT)	rclA ←	putative pyridine nucleotide‑disulfide oxidoreductase RclA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	319,759	0	C	A	100.0%	102.9 / NA	32	K81N (AAG→AAT)	rclA	putative pyridine nucleotide‑disulfide oxidoreductase RclA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/16);  total (16/16)

AAAATTTTCTCGCCATGAATTTCCAGATTTCCCTCAGGCCGATGAACACGCAGGCTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGTCCCGCCATACA  >  NC_000913/319631‑319889                                                                                                                                 |                                                                                                                                   aaaaTTTTCTCGCCATGAATTTCCAGATTTCCCTCAGGCCGATGAACACGCAGGCTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTaa                                                                                                                         >  2:435326/1‑140 (MQ=255)  aaaTTTTCTCGCCATGAATTTCCAGATTTCCCTCAGGCCGATGAACACGCAGGCTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTaaa                                                                                                                        >  2:252292/1‑140 (MQ=255)                                       ccGATGAACACGCAGGCTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGa                                                                                   >  1:136596/1‑140 (MQ=255)                                                       cTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCt                                                                           <  2:451652/132‑1 (MQ=255)                                                       cTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCt                                                                           >  1:451652/1‑132 (MQ=255)                                                                ttGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGtgctg                                                                   <  2:378133/131‑1 (MQ=255)                                                                ttGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGtgctg                                                                   >  1:378133/1‑131 (MQ=255)                                                                ttGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTc                                                          >  1:373964/1‑140 (MQ=255)                                                                ttGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTc                                                          >  1:55611/1‑140 (MQ=255)                                                                  gATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCAt                                                        >  2:234554/1‑140 (MQ=255)                                                                    tAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTc                                                          >  1:157186/1‑136 (MQ=255)                                                                    tAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTc                                                          <  2:157186/136‑1 (MQ=255)                                                                    tAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGa                                                      >  2:36846/1‑140 (MQ=255)                                                                        cTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCa                                                  <  1:490312/140‑1 (MQ=255)                                                                           cGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATg                                               >  2:7667/1‑140 (MQ=255)                                                                           cGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATg                                               >  1:452195/1‑140 (MQ=255)                                                                              cTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCGCTGCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGttt                                            <  2:490907/140‑1 (MQ=255)                                                                                 gCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTttgg                                         >  2:242688/1‑140 (MQ=255)                                                                                  ccGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTttggt                                        >  2:297877/1‑140 (MQ=255)                                                                                       gATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGa                                   <  1:36846/140‑1 (MQ=255)                                                                                         tCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATg                                 <  2:103515/140‑1 (MQ=255)                                                                                         tCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATg                                 <  1:234554/140‑1 (MQ=255)                                                                                              tCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            <  1:252292/140‑1 (MQ=255)                                                                                              tCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            <  2:447080/140‑1 (MQ=255)                                                                                              tCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            <  1:242688/140‑1 (MQ=255)                                                                                              tCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            <  2:499661/140‑1 (MQ=255)                                                                                              tCGATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            <  1:435326/140‑1 (MQ=255)                                                                                                gATATTGGGCATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGa                          <  2:55611/140‑1 (MQ=255)                                                                                                         cATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            >  2:398383/1‑129 (MQ=255)                                                                                                         cATATCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGcc                            <  1:398383/129‑1 (MQ=255)                                                                                                            atCCGCAAGATTATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAgg              >  2:268800/1‑140 (MQ=255)                                                                                                                      ttATGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGAAAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGTCCCGCCATa    <  1:268800/140‑1 (MQ=255)                                                                                                                        aTGAAAATTATTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGTCCCGCCATACa  <  1:297877/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                   AAAATTTTCTCGCCATGAATTTCCAGATTTCCCTCAGGCCGATGAACACGCAGGCTATGATTATTGATAAACTCCGCCTGGCCGTCGATCACGTCGATATTGGGCATATCCGCAAGATTATGAAAATTCTTATTACGTAAAAAATTAACCACTTCATTTTTACGCTGTATGGCACGGACAAAATCTGTGTGCTGCTGTGCGTCATGAACCAATGTTTTGGTTGGGATGCAGCCGATATTAATACAGGTCCCGCCATACA  >  NC_000913/319631‑319889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑