Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,447,873	G→T	Q93K (CAG→AAG)	rplN ←	50S ribosomal subunit protein L14

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,447,873	0	G	T	100.0%	89.0 / NA	26	Q93K (CAG→AAG)	rplN	50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/11);  total (15/11)

ATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTA  >  NC_000913/3447744‑3448000                                                                                                                                  |                                                                                                                                atcaCGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCtgttg                                                                                                                       <  1:270461/140‑1 (MQ=255)     cgacgGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCtgttgttgt                                                                                                                   >  1:391329/1‑140 (MQ=255)         ggATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCag                                                                                                               >  2:8631/1‑140 (MQ=255)                cGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAaca                                                                                                        <  1:176124/140‑1 (MQ=255)                  cTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTGCCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAAcaca                                                                                                      <  2:98221/140‑1 (MQ=255)                                    tAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCg                                                                                    <  2:258091/140‑1 (MQ=255)                                              cTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGaa                                                                          >  1:19108/1‑140 (MQ=255)                                              cTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGaa                                                                          >  1:240973/1‑140 (MQ=255)                                                      agagagATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCgg                                                                  >  1:81611/1‑140 (MQ=255)                                                            aTAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGcgac                                                             <  1:214562/139‑1 (MQ=255)                                                            aTAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGcgac                                                             >  2:214562/1‑139 (MQ=255)                                                                            tctcACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTg                                            <  2:391329/140‑1 (MQ=255)                                                                             ctcACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTgc                                           >  1:205550/1‑140 (MQ=255)                                                                                 cTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGACCCGTCCGGGCGACGAACCCCCTTCTTGGTGCGcac                                       <  2:272958/140‑1 (MQ=255)                                                                                    cGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGcaccac                                    >  2:19065/1‑140 (MQ=255)                                                                                     gAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACt                                   >  2:248216/1‑140 (MQ=255)                                                                                       aGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTAc                                 >  2:315388/1‑140 (MQ=255)                                                                                       aGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTAc                                 >  2:469763/1‑140 (MQ=255)                                                                                           cACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGcc                             >  2:37044/1‑140 (MQ=255)                                                                                             cGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCtt                           <  1:315388/140‑1 (MQ=255)                                                                                             cGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCtt                           <  1:469763/140‑1 (MQ=255)                                                                                                   aCCGGCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGcac                     >  2:238539/1‑140 (MQ=255)                                                                                                      ggCCCAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATc                  >  1:106636/1‑140 (MQ=255)                                                                                                        cccAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCAc                <  2:19108/140‑1 (MQ=255)                                                                                                          cAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCAc                >  2:171997/1‑138 (MQ=255)                                                                                                          cAAAAATACGCGTACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCAc                <  1:171997/138‑1 (MQ=255)                                                                                                                      tACCGATAGGCTTCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTa  <  1:248216/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                ATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTA  >  NC_000913/3447744‑3448000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑