Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,747,397	G→T	R105L (CGG→CTG)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,747,397	0	G	T	100.0%	86.6 / NA	25	R105L (CGG→CTG)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/11);  total (14/11)

TGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGC  >  NC_000913/3747268‑3747530                                                                                                                                  |                                                                                                                                      tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAGTGCTATTTTGTCCTCGGACCGCCTGGCGATGGaa                                                                                                                             >  2:101063/1‑140 (MQ=255)   cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAAt                                                                                                                           >  1:471909/1‑140 (MQ=255)               ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTg                                                                                                               <  2:149207/140‑1 (MQ=255)                gtgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTgg                                                                                                              >  1:22874/1‑140 (MQ=255)                                     tCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGaa                                                                                         >  1:434271/1‑140 (MQ=255)                                            cGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAActct                                                                                  <  2:35875/140‑1 (MQ=255)                                                        acaGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGAccc                                                                      >  1:387607/1‑140 (MQ=255)                                                           gggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGAc                                                                   <  1:462719/140‑1 (MQ=255)                                                           gggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGAc                                                                   >  1:467350/1‑140 (MQ=255)                                                            ggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACa                                                                  >  2:129409/1‑140 (MQ=255)                                                            ggAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACa                                                                  >  2:161654/1‑140 (MQ=255)                                                             gAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACaa                                                                 <  1:92911/140‑1 (MQ=255)                                                             gAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACaa                                                                 <  2:22874/140‑1 (MQ=255)                                                                      tGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTCTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTgg                                                        >  2:458294/1‑140 (MQ=255)                                                                        tttttGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGAc                                                      <  1:161654/140‑1 (MQ=255)                                                                            tGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCggg                                                  >  1:417187/1‑140 (MQ=255)                                                                                  aTAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCg                                            >  1:289755/1‑140 (MQ=255)                                                                                                ccGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                              <  2:471909/140‑1 (MQ=255)                                                                                                ccGCTCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                              <  2:467350/140‑1 (MQ=255)                                                                                                    tCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                              >  1:185479/1‑136 (MQ=255)                                                                                                    tCGGGAATGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACg                              <  2:185479/136‑1 (MQ=255)                                                                                                           tGCTATTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAag                   >  2:152962/1‑140 (MQ=255)                                                                                                               aTTTTGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCa               <  2:417187/140‑1 (MQ=255)                                                                                                                 tttGTCCTCGGACCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACg             <  1:458294/140‑1 (MQ=255)                                                                                                                            aCCGCCTGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGc  >  2:83611/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                      TGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTGGCAGGAAGATGGCATCCCGGAAAAACTCTACCCGCTGACCCGACAAACCTTGTGGACCGGGCATCCGGTGTCGCTGTTACGCTGGCTGAAAGAGCACGAACCAGAACGC  >  NC_000913/3747268‑3747530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑