Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,758,721	C→T	A381T (GCG→ACG)	selB ←	selenocysteyl‑tRNA‑specific translation elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,758,721	0	C	T	100.0%	59.0 / NA	18	A381T (GCG→ACG)	selB	selenocysteyl‑tRNA‑specific translation elongation factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

TTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGC  >  NC_000913/3758595‑3758855                                                                                                                               |                                                                                                                                       ttttCCGCTGCCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAAccgcg                                                                                                                           <  1:37798/140‑1 (MQ=255)    tCCGCTGCCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAAccgcgccg                                                                                                                        <  1:159859/140‑1 (MQ=255)              gcgggcggCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGAt                                                                                                              <  1:247126/140‑1 (MQ=255)                                   caacaaGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACgcatcggcat                                                                                         <  2:60551/140‑1 (MQ=255)                                              aaCCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGcc                                                                              >  2:461761/1‑140 (MQ=255)                                                             aaCCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACgcc                                                               <  2:392388/140‑1 (MQ=255)                                                                  ggCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACgccagcca                                                          >  1:80354/1‑140 (MQ=255)                                                                  ggCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACgccagcca                                                          >  2:367376/1‑140 (MQ=255)                                                                   gCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGGGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCAt                                                         >  2:414971/1‑140 (MQ=255)                                                                             caATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATAtt                                               >  1:14344/1‑140 (MQ=255)                                                                                 tCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCgg                                           >  1:323645/1‑140 (MQ=255)                                                                                                 cGTTGAGCTGGCGCGACCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGa                           <  1:367376/140‑1 (MQ=255)                                                                                                   ttGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTAc                                      <  1:25891/127‑1 (MQ=255)                                                                                                   ttGAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTAc                                      >  2:25891/1‑127 (MQ=255)                                                                                                     gAGCTGGCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGAcgcg                       <  1:461761/140‑1 (MQ=255)                                                                                                          ggCGCGCCCAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCggg                  <  2:323645/140‑1 (MQ=255)                                                                                                                cccAGGCGAAATCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGc            >  1:445283/1‑140 (MQ=255)                                                                                                                          aTCCGTAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGAcgc  <  2:295199/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       TTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGC  >  NC_000913/3758595‑3758855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑