Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,245,598	C→A	P274P (CCG→CCT)	malE ←	maltose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,245,598	0	C	A	100.0%	108.9 / NA	32	P274P (CCG→CCT)	malE	maltose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/18);  total (14/18)

ACCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTCGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCA  >  NC_000913/4245464‑4245727                                                                                                                                       |                                                                                                                                  aCCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAg                                                                                                                              <  1:160123/140‑1 (MQ=255) aCCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAg                                                                                                                              <  1:317829/140‑1 (MQ=255) aCCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAg                                                                                                                              <  1:1053/140‑1 (MQ=255)    gCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTAc                                                                                                                           >  1:82863/1‑140 (MQ=255)         cAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTa                                                                                                                      <  2:474147/140‑1 (MQ=255)         cAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTa                                                                                                                      <  1:384732/140‑1 (MQ=255)             ccTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTa                                                                                                                      >  1:181444/1‑136 (MQ=255)             ccTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTa                                                                                                                      <  2:181444/136‑1 (MQ=255)                         agcagATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACAcc                                                                                                                  >  2:24266/1‑128 (MQ=255)                         agcagATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACAcc                                                                                                                  <  1:24266/128‑1 (MQ=255)                           cagATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGc                                                                                                    >  2:151126/1‑140 (MQ=255)                           cagATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGGTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGc                                                                                                    >  2:96359/1‑140 (MQ=255)                              aTAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTgg                                                                                                 >  1:53109/1‑140 (MQ=255)                                 gTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGgtgt                                                                                              <  2:53109/140‑1 (MQ=255)                                 gTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGgtgt                                                                                              <  2:170731/140‑1 (MQ=255)                                 gTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGgtgt                                                                                              <  2:309408/140‑1 (MQ=255)                                     tCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGAt                                                                                          >  1:340844/1‑140 (MQ=255)                                                        gCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACggg                                                                       >  1:95300/1‑140 (MQ=255)                                                                                     ccTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCttt                                          <  2:155927/140‑1 (MQ=255)                                                                                         cgcTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTttatta                                      >  1:317871/1‑140 (MQ=255)                                                                                         cgcTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTttatta                                      <  1:151126/140‑1 (MQ=255)                                                                                              aGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGc                                 >  1:436224/1‑140 (MQ=255)                                                                                              aGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGc                                 >  1:229585/1‑140 (MQ=255)                                                                                                cACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAg                               <  1:407383/140‑1 (MQ=255)                                                                                                cACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAg                               >  1:350533/1‑140 (MQ=255)                                                                                                        cgaacgGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTaaa                                    <  1:141571/127‑1 (MQ=255)                                                                                                        cgaacgGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTaaa                                    >  2:141571/1‑127 (MQ=255)                                                                                                        cgaacgGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCg                       <  1:429514/140‑1 (MQ=255)                                                                                                            cgGTTTGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATgg                   <  2:172130/140‑1 (MQ=255)                                                                                                               tttGGATGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGt                >  1:177757/1‑140 (MQ=255)                                                                                                                    aTGGTTGACCCTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCg           <  2:177757/140‑1 (MQ=255)                                                                                                                             ccTTGAAGGTAGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCa  <  2:317871/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  ACCGCTTCCAGACCTTCATCAGTCAGCAGATAGTTTTCGAGGAACTCTTTCGCCAGCTCTTTGTTCGGACTGGCGGCGTTAATACCTGCGCTCAGCACGCCAACGAACGGTTTGGATGGTTGACCCTTGAAGGTCGGCAGTACCGTTACACCATAATTCACTTTGCTGGTGTCGATGTTGGACCATGCCCACGGGCCGTTGATGGTCATCGCTGTTTCGCCTTTATTAAAGGCAGCTTCTGCGATGGAGTAATCGGTGTCTGCA  >  NC_000913/4245464‑4245727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑