Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	331,384	A→G	Y641C (TAC→TGC)	betT →	choline:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	331,384	0	A	G	100.0%	158.8 / NA	43	Y641C (TAC→TGC)	betT	choline:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/16);  total (27/16)

TGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCC  >  NC_000913/331253‑331519                                                                                                                                    |                                                                                                                                         tGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAag                                                                                                                                  >  2:342062/1‑140 (MQ=255) tGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAag                                                                                                                                  >  2:382331/1‑140 (MQ=255)   tCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAagag                                                                                                                                <  1:342062/140‑1 (MQ=255)        cAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGt                                                                                                                           >  2:487757/1‑140 (MQ=255)           aTTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGAt                                                                                                                        <  2:149429/140‑1 (MQ=255)           aTTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGAt                                                                                                                        >  2:460397/1‑140 (MQ=255)            tttGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATc                                                                                                                       <  1:487757/140‑1 (MQ=255)                 ccGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGa                                                                                                                  <  2:55418/140‑1 (MQ=255)                 ccGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGa                                                                                                                  >  2:498472/1‑140 (MQ=255)                 ccGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGa                                                                                                                  >  1:271044/1‑140 (MQ=255)                     agcaATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATAtt                                                                                                              >  2:77242/1‑140 (MQ=255)                         atatTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTg                                                                                                          <  1:498472/140‑1 (MQ=255)                               ggTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAga                                                                                                    >  2:105826/1‑139 (MQ=255)                               ggTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGt                                                                                                    >  1:455668/1‑140 (MQ=255)                                gTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTa                                                                                                   <  1:275077/140‑1 (MQ=255)                                 tGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCg                                                                                                                   >  1:451792/1‑123 (MQ=255)                                 tGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCg                                                                                                                   <  2:451792/123‑1 (MQ=255)                                     gggCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑Gc                                                                                             <  2:271044/140‑1 (MQ=255)                                            acctaccGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCt                                                                                      <  1:382331/140‑1 (MQ=255)                                               taccGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTaa                                                                                   <  2:74671/140‑1 (MQ=255)                                                  cgcgCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCg                                                                                            >  1:398974/1‑128 (MQ=255)                                                  cgcgCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCg                                                                                            <  2:398974/128‑1 (MQ=255)                                                      cacgcaGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTAtt                                                                            >  1:478405/1‑140 (MQ=255)                                                         gcaGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCAt                                                                         >  1:321329/1‑140 (MQ=255)                                                             cGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTcc                                                                     >  1:447796/1‑140 (MQ=255)                                                                       aCCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAgc                                                           >  1:160699/1‑140 (MQ=255)                                                                       aCCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAgc                                                           >  1:106321/1‑140 (MQ=255)                                                                       aCCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAgc                                                           >  1:288150/1‑140 (MQ=255)                                                                           actacCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCg                                                       <  2:455668/140‑1 (MQ=255)                                                                              acCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGc                                                    >  1:220166/1‑140 (MQ=255)                                                                               ccGGCTGGAACCCTTCCTGTTAAAGGGCACCCGGGCCACCGACCGAAGGGACTGAAGAAAAAGGAAGGT‑ATCACCCAATTTTCTGGCCAGGACCAGGCGGC‑CCTTTACTTTATTCATCTCCATCGTGAAGCGCCGGGCCa                                                  <  2:210631/140‑1 (MQ=255)                                                                                       aaaCCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTg                                           <  2:450196/140‑1 (MQ=255)                                                                                                tGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCg                                  >  2:209765/1‑140 (MQ=255)                                                                                                tGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCg                                  >  2:426977/1‑140 (MQ=255)                                                                                                     gAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTg                                           <  1:479278/126‑1 (MQ=255)                                                                                                     gAAGGCAGCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTg                                           >  2:479278/1‑126 (MQ=255)                                                                                                            gCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTg                      >  2:447265/1‑140 (MQ=255)                                                                                                            gCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTg                      >  1:257335/1‑140 (MQ=255)                                                                                                            gCCAGGGCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTg                      >  2:345531/1‑140 (MQ=255)                                                                                                                  gCAACGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTg                >  1:202617/1‑140 (MQ=255)                                                                                                                      cGACCTGATGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATa            >  2:264616/1‑140 (MQ=255)                                                                                                                             aTGGACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACcgc     >  2:313849/1‑140 (MQ=255)                                                                                                                               ggACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCgcgc   <  1:264616/140‑1 (MQ=255)                                                                                                                                gACTGCAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGcc  <  2:37437/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         TGTCTATCAGATTTGGCCGCAGCAATATTCGGTGCCGGGCTTTACCTACCGCGCACGCAGCGGTAAATCGACCTACTACCGGCTGGAAACCTTCCTGTTAGAAGGCAGCCAGGGCAACGACCTGATGGACTACAGCAAAGAGCAGGTGATCACCGATATTCTTGACCAGTACGA‑GCGGCACCTTAACTTTATTCATCTCCATCGTGAAGCGCCGGGCCATAGCGTGATGTTCCCGGACGCGTGATTGTTATTGCATAAAACCGCGCC  >  NC_000913/331253‑331519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑