Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	793,209	G→T	R194S (CGC→AGC)	modF ←	ABC family protein ModF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	793,209	0	G	T	100.0%	101.7 / NA	30	R194S (CGC→AGC)	modF	ABC family protein ModF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/19);  total (11/19)

AGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCG  >  NC_000913/793079‑793343                                                                                                                                   |                                                                                                                                       aGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCAc                                                                                                                               <  1:166425/140‑1 (MQ=255)      ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCAc                                                                                                                               >  2:243026/1‑135 (MQ=255)      ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCAc                                                                                                                               <  1:243026/135‑1 (MQ=255)      ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTa                                                                                                                          >  2:491377/1‑140 (MQ=255)      ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTa                                                                                                                          >  1:463565/1‑140 (MQ=255)       tCACTATGCGCCCATTGCGCAACGACTACTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTAc                                                                                                                         <  2:498083/140‑1 (MQ=255)       tCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTAc                                                                                                                         <  2:386245/140‑1 (MQ=255)       tCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTAc                                                                                                                         <  2:124170/140‑1 (MQ=255)              gcgcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTa                                                                                                                  >  2:324204/1‑140 (MQ=255)              gcgcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTa                                                                                                                  >  1:223131/1‑140 (MQ=255)                gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAAt                                                                                                                >  2:255417/1‑140 (MQ=255)                      tGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGa                                                                                                          <  1:120428/140‑1 (MQ=255)                       gcgcGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGAc                                                                                                         <  2:314114/140‑1 (MQ=255)                                      gttgGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCg                                                                                          <  2:403970/140‑1 (MQ=255)                                       ttgGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGa                                                                                         <  2:482452/140‑1 (MQ=255)                                           aGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCg                                                                                     >  2:381691/1‑140 (MQ=255)                                                 tCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTcagc                                                                               <  2:221796/140‑1 (MQ=255)                                                  cccctTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTcagcc                                                                              <  1:290860/137‑1 (MQ=255)                                                       ttAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAgctg                                                                         <  1:381691/140‑1 (MQ=255)                                                        tAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAgctgc                                                                        <  1:255417/140‑1 (MQ=255)                                                           cgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGa                                                                     >  1:382669/1‑140 (MQ=255)                                                                           cGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCa                                                     <  2:223131/140‑1 (MQ=255)                                                                                        agcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACgg                                        <  2:463565/140‑1 (MQ=255)                                                                                               cagcaAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGa                                            >  2:150261/1‑129 (MQ=255)                                                                                               cagcaAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGa                                            <  1:150261/129‑1 (MQ=255)                                                                                                agcaAACTGGACAAACTCCGGGATCTCATCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACAGCTCATCaa                                <  1:373315/140‑1 (MQ=255)                                                                                                                           aTCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCa     <  1:324204/140‑1 (MQ=255)                                                                                                                            tCGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAg    >  2:131450/1‑140 (MQ=255)                                                                                                                             cGAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAgc   >  2:407987/1‑140 (MQ=255)                                                                                                                              gAAGCTATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAgcg  <  1:131450/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       AGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCG  >  NC_000913/793079‑793343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑