Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,967,611	(C)5→4	coding (891/1050 nt)	cheB ←	protein‑glutamate methylesterase/protein glutamine deamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,967,607	0	C	.	100.0%	268.7 / NA	64	coding (895/1050 nt)	cheB	protein‑glutamate methylesterase/protein glutamine deamidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (44/20);  total (44/20)

TCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGCCCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGT  >  NC_000913/1967470‑1967741                                                                                                                                           |                                                                                                                                        tCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGccc                                                                                                                                       >  1:248918/1‑140 (MQ=255) tCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGccc                                                                                                                                       >  1:1505148/1‑140 (MQ=255) tCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGccc                                                                                                                                       >  1:1489990/1‑140 (MQ=255) tCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGccc                                                                                                                                       >  2:227023/1‑140 (MQ=255)     gCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGccccgcc                                                                                                                                   >  2:1821078/1‑137 (MQ=255)           aTTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGc                                                                                                                            >  1:753051/1‑140 (MQ=255)             ttttGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCAt                                                                                                                          <  1:1595624/140‑1 (MQ=255)                 cccAACATTTGCTGGCTTACCTGGCTAAGATCGCCCACTTCCCAGACCCCACCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGcc                                                                                                                      <  2:352940/139‑1 (MQ=255)                                tttACCTGGCTAAGAGCGCCCACTTCCCAGCCCCCACCCATATGGATGGCCTCGCGCGGCATCCCAAACCCCACGCACCTTGC‑TTCGTTTGGCGCAAGGGCCCAT‑CCCCCGCCTGACGCATCCCCAA‑CATTCCcgccgcg                                                                                                      <  2:83504/139‑1 (MQ=255)                                cTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCcgccgcg                                                                                                      <  1:1094709/140‑1 (MQ=255)                                cTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCcgccgcg                                                                                                      >  2:150325/1‑140 (MQ=255)                                   aCCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCGTCGCCAA‑CATTCCcgccgcgccg                                                                                                   >  2:809118/1‑140 (MQ=255)                                     cTGGCTAAGATCGCCCCCTTCGCAGACACCCCCCATTTTGATGCCCTCGCGCGGCATGCCGAACCCCACGCAACTTGC‑TTCGTTTTGCGCAGGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATCCCCGCCGCGCcgtc                                                                                                 <  2:369249/140‑1 (MQ=255)                                     cTGGCTAAGATCGACCACTTCGCAGACCCCACCCATATTGAGGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCcgtc                                                                                                 <  2:1380532/140‑1 (MQ=255)                                      tggtaaaGATCGACCACTCCCCAGACCCCACCCATATTGACGGCCTCGCGCGCCATGCCGAACACCACGCACTTTCC‑TTCTTTTTGCCCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCcgtcg                                                                                                <  2:1266560/135‑1 (MQ=255)                                      tGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCcgtcg                                                                                                <  1:209143/140‑1 (MQ=255)                                        gCTAAGATCGACCACTCCGCAGACCCCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTGGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGtt                                                                                              <  1:651437/140‑1 (MQ=255)                                         cTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTg                                                                                             >  1:421787/1‑140 (MQ=255)                                         cTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTg                                                                                             >  1:350270/1‑140 (MQ=255)                                         cTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTg                                                                                             >  2:1213654/1‑140 (MQ=255)                                         cTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTg                                                                                             >  2:1310119/1‑140 (MQ=255)                                          tAAGATCGACCACTTCGCAGACCCCCCCCATATTGAGGCCCTCGCGGGGCATGCCGAACCCCACCCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGc                                                                                            <  2:1144923/140‑1 (MQ=255)                                          tAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGc                                                                                            <  1:150325/140‑1 (MQ=255)                                               tCGACCACTTCGCAGACCCCCCCCATATTGATGGCCCCGCGCGGCATGCCGAACCCCACGCACCTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATa                                                                                       <  2:1249738/140‑1 (MQ=255)                                               tCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATa                                                                                       <  2:533757/140‑1 (MQ=255)                                                cGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATAc                                                                                      <  1:1213654/140‑1 (MQ=255)                                                  aCCACTTCGTAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCg                                                                                    >  2:609428/1‑140 (MQ=255)                                                  aCCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCg                                                                                    >  1:1296559/1‑140 (MQ=255)                                                  aCCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCg                                                                                    >  1:98042/1‑140 (MQ=255)                                                  aCCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCg                                                                                    >  1:582313/1‑140 (MQ=255)                                                  aCCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCg                                                                                    >  1:1530017/1‑140 (MQ=255)                                                    cACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGt                                                                                  <  1:615347/140‑1 (MQ=255)                                                      cTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCa                                                                                >  2:1417701/1‑140 (MQ=255)                                                       ttCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAg                                                                               >  1:1498380/1‑140 (MQ=255)                                                         cGCAGACCCCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGa                                                                             <  1:737155/140‑1 (MQ=255)                                                         cGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGa                                                                             >  2:645550/1‑140 (MQ=255)                                                              acacCACCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCAcc                                                                        <  1:627497/140‑1 (MQ=255)                                                                 ccaccCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCa                                                                     >  1:1332433/1‑140 (MQ=255)                                                                  caccCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCaa                                                                    >  2:989439/1‑140 (MQ=255)                                                                   accCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAAc                                                                   >  1:1581242/1‑140 (MQ=255)                                                                   accCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAAc                                                                   <  2:350270/140‑1 (MQ=255)                                                                    cccATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAAcc                                                                  >  1:1580901/1‑140 (MQ=255)                                                                        tatTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAGGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAt                                                              <  1:1005572/140‑1 (MQ=255)                                                                          ttGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTa                                                            >  2:133434/1‑140 (MQ=255)                                                                          ttGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTa                                                            >  2:1124085/1‑140 (MQ=255)                                                                          ttGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTa                                                            >  2:795225/1‑140 (MQ=255)                                                                          ttGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTa                                                            <  1:172513/140‑1 (MQ=255)                                                                             agggCCGCGCGCGCCACCCCGAAAACCACCCATCTTGC‑TCCGTTTGACGCAGGGTTCCA‑CCCCCCGCTTGGCG‑ATGCCCAACCATTCCCGCCGCGCCGTCGTTGCCCATACAGGTCAGGATCACCCCAACCGCATTACGc                                                         <  2:753051/138‑1 (MQ=255)                                                                             aTGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGc                                                         >  2:1514440/1‑140 (MQ=255)                                                                               ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAt                                                              <  2:607187/133‑1 (MQ=255)                                                                               ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAt                                                              >  2:607636/1‑133 (MQ=255)                                                                               ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAt                                                              <  1:607636/133‑1 (MQ=255)                                                                               ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAt                                                              >  1:607187/1‑133 (MQ=255)                                                                               ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGccc                                                       >  2:690620/1‑140 (MQ=255)                                                                               ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCAAG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGccc                                                       >  2:854403/1‑140 (MQ=255)                                                                                gCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGcccc                                                      >  2:731644/1‑140 (MQ=255)                                                                                gCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGcccc                                                      >  1:1022396/1‑140 (MQ=255)                                                                                  cTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGc                                                    >  1:1230137/1‑140 (MQ=255)                                                                                  cTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGc                                                    >  1:319425/1‑140 (MQ=255)                                                                                  cTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGc                                                    >  2:1182606/1‑140 (MQ=255)                                                                                    cgcgcgGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCt                                                  >  1:704958/1‑140 (MQ=255)                                                                                    cgcgcgGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGAATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCt                                                  >  1:553054/1‑140 (MQ=255)                                                                                     gcgcgGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACTGCATTACGCCCCGCCTg                                                 >  2:535248/1‑140 (MQ=255)                                                                                        gggCATCCCGAACCCCACGCAACTTGC‑TTCTTTTTGCGCAGGGGTCCAT‑CCCCCGCCTGACGCACCGCCAA‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGttt                                              <  2:1489990/139‑1 (MQ=255)                                                                                             tGCCGACCCCCCCGCAACTTGC‑TTCTTTTTGCGCAGGGGTCCATG‑CCCCGCCTGACGCACCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGa                                         <  2:1230137/140‑1 (MQ=255)                                                                                             tGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGa                                         <  1:1417701/140‑1 (MQ=255)                                                                                                    ccccccGCACCTTGC‑TTCTTTTTGCGCAGGGGTCCAT‑CCCCCGCCTGACGCACCGCCAC‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATg                                  <  2:1581242/135‑1 (MQ=255)                                                                                                    ccccccGCAACTTGC‑TTCTTTTTGCG‑AAGGGGCCCTTCCCCCGCCTGACGCACCGCCAA‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATg                                  <  2:1505148/135‑1 (MQ=255)                                                                                                     cccacGCAACTTCC‑TTCGTTTGGCGCAGGGGCCCA‑GCCCCCGCCTGACGCACCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATgg                                 <  2:1530017/139‑1 (MQ=255)                                                                                                       ccccaaccttcc‑ttcTTTTTGCGCAGGGGCCCATG‑CCCCGCCTGACGAATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaa                               <  2:553054/133‑1 (MQ=255)                                                                                                        ccccaccTTGC‑TTCTTTTTGCGAAGGGGTCCAT‑CCCCCGCCTGACCCATCCCCAC‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaac                              <  2:1296559/134‑1 (MQ=255)                                                                                                        acGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaac                              >  2:1451912/1‑140 (MQ=255)                                                                                                         cGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaaca                             >  2:1358709/1‑140 (MQ=255)                                                                                                         cGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaaca                             >  1:810871/1‑140 (MQ=255)                                                                                                         cGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaaca                             >  1:50697/1‑140 (MQ=255)                                                                                                          gCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaa                            >  2:1554611/1‑140 (MQ=255)                                                                                                          ccAACTTGC‑TTCGTTTTGCGCAAGGGTCCAT‑CCCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaa                            <  1:996390/139‑1 (MQ=255)                                                                                                           gaaCTTGCTTTCGTTTGGC‑CAAGGGGCCCTTCCCCCGCTTGACGCTGCGCCAA‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCTCCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaa                            <  2:1307288/139‑1 (MQ=255)                                                                                                               ttGC‑TTCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACAt                       >  2:1279839/1‑140 (MQ=255)                                                                                                                  c‑TTCGTTTTGCGCAGGGGTCCATG‑CCCCGCCTGACGCATCGCAAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTa                    <  1:49021/140‑1 (MQ=255)                                                                                                                   tttCTTTTTCCGCAGGGGCCCA‑GCCCCCGCCTGACGCATCCCCAA‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTa                    <  2:1580901/139‑1 (MQ=255)                                                                                                                     tCGTTTTGCGCAAGGGTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTAcc                  >  1:1418749/1‑140 (MQ=255)                                                                                                                      ctttttGCCCAGGGGTCCTTGCCCCC‑CCTGACGCACCGCCAA‑CATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCg                 <  2:1560946/138‑1 (MQ=255)                                                                                                                                  gggTCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGAc     >  2:1813366/1‑140 (MQ=255)                                                                                                                                     tCCATG‑CCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGt  >  2:778584/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        TCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGC‑TTCGTTTTGCGCAAGGGTCCATGCCCCCGCCTGACGCATCGCCAA‑CATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGT  >  NC_000913/1967470‑1967741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑