BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,385,869	(A)_7→8	coding (10/972 nt)	yfbL →	putative peptidase YfbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,385,862	1	.	A	99.2%	447.9 / ‑4.0	125	coding (3/972 nt)	yfbL	putative peptidase YfbL
Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (76/48); total (77/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 131 total aligned reads displayed.

AAAATCCTTTTAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCG  >  NC_000913/2385725‑2385996

aaaaTCCTTTTAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑aa                                                                                                                                      >  1:153204/1‑140 (MQ=255)

     ccTTTTAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑aaaaaaa                                                                                                                                 >  2:909283/1‑140 (MQ=255)

        tttAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACTCAACCCATTAATAATTTGCATATG‑aaaaaaaata                                                                                                                              >  1:214742/1‑137 (MQ=255)

        tttAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑aaaaaaaata                                                                                                                              >  1:720376/1‑137 (MQ=255)

        tttAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑aaaaaaaata                                                                                                                              >  2:1347741/1‑137 (MQ=255)

            aataaaCTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCt                                                                                                                           <  1:128673/140‑1 (MQ=255)

                aaCTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGc                                                                                                                       <  1:1588734/140‑1 (MQ=255)

                 aCTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCt                                                                                                                      >  1:1129661/1‑140 (MQ=255)

                    cAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCtttt                                                                                                                   <  1:311859/140‑1 (MQ=255)

                      ggCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTttat                                                                                                                 >  1:1793851/1‑140 (MQ=255)

                       gCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTttatt                                                                                                                >  2:133938/1‑140 (MQ=255)

                             gACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCt                                                                                                                      >  2:764680/1‑128 (MQ=255)

                             gACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCt                                                                                                                      <  1:764680/128‑1 (MQ=255)

                                 tAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTCCTTTTATTATATTATTtg                                                                                                      >  2:1268363/1‑140 (MQ=255)

                                  aaaaTATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTtgt                                                                                                     >  2:907365/1‑140 (MQ=255)

                                     atatACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGtt                                                                                                  <  1:92859/140‑1 (MQ=255)

                                        taCTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGttttt                                                                                               >  1:800544/1‑140 (MQ=255)

                                         aCTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTa                                                                                              >  1:166297/1‑140 (MQ=255)

                                            ctTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATTATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTc                                                                                           >  1:1541043/1‑140 (MQ=255)

                                            ctTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTc                                                                                           >  1:820669/1‑140 (MQ=255)

                                            ctTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTc                                                                                           >  2:473618/1‑140 (MQ=255)

                                             tttGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTcc                                                                                          >  2:1135683/1‑140 (MQ=255)

                                             tttGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTcc                                                                                          >  2:961358/1‑140 (MQ=255)

                                               tGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCt                                                                                        >  2:1530616/1‑140 (MQ=255)

                                               tGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAA‑TTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTa                                                                                       >  1:1664419/1‑140 (MQ=255)

                                                gTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTa                                                                                       >  1:297259/1‑140 (MQ=255)

                                                    aaTCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGat                                                                                   <  2:153204/140‑1 (MQ=255)

                                                     aTCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGatt                                                                                  >  2:61747/1‑140 (MQ=255)

                                                       ccTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGattat                                                                                >  2:47639/1‑140 (MQ=255)

                                                         tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTAttt                                                                              >  2:463985/1‑140 (MQ=255)

                                                         tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTAttt                                                                              >  2:51252/1‑140 (MQ=255)

                                                         tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTAttt                                                                              >  2:803434/1‑140 (MQ=255)

                                                         tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTAttt                                                                              >  1:130340/1‑140 (MQ=255)

                                                         tGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTAttt                                                                              >  1:751341/1‑140 (MQ=255)

                                                              aTCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc                                                                         <  2:290153/140‑1 (MQ=255)

                                                              aTCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc                                                                         <  2:1129661/140‑1 (MQ=255)

                                                              aTCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc                                                                         <  1:1118604/140‑1 (MQ=255)

                                                               tCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAcc                                                                        <  2:1793851/140‑1 (MQ=255)

                                                               tCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAcc                                                                        <  1:9988/140‑1 (MQ=255)

                                                                    ataaAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAAccc                                                                   >  1:674366/1‑140 (MQ=255)

                                                                    ataaAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAAccc                                                                   <  1:1738447/140‑1 (MQ=255)

                                                                      aaaaTACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTg                                                                 <  1:1479677/140‑1 (MQ=255)

                                                                         aTACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt                                                              <  1:1268363/140‑1 (MQ=255)

                                                                         aTACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt                                                              <  2:1504281/140‑1 (MQ=255)

                                                                          tACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTc                                                             >  2:1404150/1‑140 (MQ=255)

                                                                           aCAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCa                                                            <  1:265577/140‑1 (MQ=255)

                                                                            cAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCaa                                                           <  1:1347741/140‑1 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc                                                                         >  1:1014831/1‑125 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTAc                                                                         <  2:1014831/125‑1 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAAt                                                          >  2:156272/1‑140 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAAt                                                          <  2:686466/140‑1 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAAt                                                          <  1:51252/140‑1 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAAt                                                          <  1:803434/140‑1 (MQ=255)

                                                                             aaTATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAAt                                                          >  1:686466/1‑140 (MQ=255)

                                                                               tatCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAAc                                                                     <  1:669802/127‑1 (MQ=255)

                                                                               tatCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGc                                                        >  1:1649843/1‑140 (MQ=255)

                                                                                  cGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt                                                              <  2:641881/131‑1 (MQ=255)

                                                                                  cGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCAtt                                                     >  1:619776/1‑140 (MQ=255)

                                                                                    aaaCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTAc                                                   >  2:232003/1‑140 (MQ=255)

                                                                                    aaaCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTAc                                                   >  2:1278275/1‑140 (MQ=255)

                                                                                    aaaCCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTAc                                                   >  2:1447894/1‑140 (MQ=255)

                                                                                         cATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCa                                              >  2:1376057/1‑140 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt                                                              >  2:377842/1‑122 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt                                                              <  2:749232/122‑1 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGt                                                              <  1:377842/122‑1 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAAc                                            >  1:634923/1‑140 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAAc                                            >  2:310492/1‑140 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAAc                                            >  2:1345453/1‑140 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAAc                                            >  1:1538194/1‑140 (MQ=255)

                                                                                           tAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAAc                                            >  1:744363/1‑140 (MQ=255)

                                                                                            aGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACg                                           <  2:674366/140‑1 (MQ=255)

                                                                                                  taatGATTCAACAACGCAACCCATTAATAATTTGCATGTGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa                                     <  1:1161883/140‑1 (MQ=255)

                                                                                                  taatGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa                                     <  1:1775676/140‑1 (MQ=255)

                                                                                                  taatGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa                                     <  2:619776/140‑1 (MQ=255)

                                                                                                  taatGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa                                     <  1:473618/140‑1 (MQ=255)

                                                                                                  taatGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATACATTACCGTCAACGCCCCGa                                     <  1:726785/140‑1 (MQ=255)

                                                                                                  taatGATTCAACAACGCAACCCATCAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGa                                     <  1:98689/140‑1 (MQ=255)

                                                                                                      gATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACg                                 >  1:880093/1‑140 (MQ=255)

                                                                                                       aTTCAACAACTCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGc                                <  2:214742/140‑1 (MQ=255)

                                                                                                       aTTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGc                                <  2:384478/140‑1 (MQ=255)

                                                                                                         tCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCaa                              <  2:1700260/140‑1 (MQ=255)

                                                                                                           aacaacGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGt                            >  2:583449/1‑140 (MQ=255)

                                                                                                           aacaacGAAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGt                            >  2:709980/1‑140 (MQ=255)

                                                                                                             caacGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTcc                          >  2:1171843/1‑140 (MQ=255)

                                                                                                                cGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGa                       >  2:1023275/1‑140 (MQ=255)

                                                                                                                cGCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGa                       >  2:577860/1‑140 (MQ=255)

                                                                                                                 gCAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAg                      >  2:199415/1‑140 (MQ=255)

                                                                                                                  cAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGc                     >  1:425325/1‑140 (MQ=255)

                                                                                                                  cAACCCATTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGc                     >  2:1108486/1‑140 (MQ=255)

                                                                                                                        aTTAATAATTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAg               >  1:615424/1‑140 (MQ=255)

                                                                                                                          taataaTTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAg                      >  2:404941/1‑131 (MQ=255)

                                                                                                                          taataaTTTGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGaa             >  2:1239984/1‑140 (MQ=255)

                                                                                                                                 ttGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAg      <  2:274295/140‑1 (MQ=255)

                                                                                                                                 ttGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAg      >  1:752672/1‑140 (MQ=255)

                                                                                                                                  tGCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGt     >  1:1831850/1‑140 (MQ=255)

                                                                                                                                   gCATATGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGtt    >  1:1456183/1‑140 (MQ=255)

                                                                                                                                     atatGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg  <  2:579047/140‑1 (MQ=255)

                                                                                                                                     atatGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg  <  2:166297/140‑1 (MQ=255)

                                                                                                                                     atatGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg  <  1:47639/140‑1 (MQ=255)

                                                                                                                                     atatGAAAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCg  <  2:1119544/140‑1 (MQ=255)

AAAATCCTTTTAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATG‑AAAAAAATAATCTTTGCTTTTATTATATTATTTGTGTTTTTACTCCCTATGATTATTTTTTACCAACCCTGGGTCAATGCATTACCGTCAACGCCCCGACACGCAAGTCCTGAGCAATTAGAAAAAACAGTTCG  >  NC_000913/2385725‑2385996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`