Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,982,684	(C)8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,982,676	1	.	C	100.0%	174.2 / NA	49	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/24);  total (25/24)

TGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982537‑3982809                                                                                                                                             |                                                                                                                                       tGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGt                                                                                                                                         >  1:188609/1‑140 (MQ=255)   gATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑cc                                                                                                                                     >  1:1061574/1‑140 (MQ=255)     ttGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑cccc                                                                                                                                   <  2:816049/140‑1 (MQ=255)        tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:1450617/1‑140 (MQ=255)        tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:1460080/1‑140 (MQ=255)        tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:1536279/1‑140 (MQ=255)        tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:346933/1‑140 (MQ=255)        tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑cccc                                                                                                                                   <  2:323996/137‑1 (MQ=255)        tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑cccc                                                                                                                                   >  1:323996/1‑137 (MQ=255)           ttCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccct                                                                                                                             <  2:1693822/140‑3 (MQ=255)              cAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑cccccccccctcg                                                                                                                          >  1:203308/1‑135 (MQ=255)                  tGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGcacca                                                                                                                       <  2:1254167/140‑1 (MQ=255)                  tGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGcacca                                                                                                                       <  1:521098/140‑1 (MQ=255)                     cGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGa                                                                                                                    <  1:801132/140‑1 (MQ=255)                          gTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTa                                                                                                               <  2:1061574/140‑1 (MQ=255)                               aaTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGaa                                                                                                          <  1:1374865/140‑1 (MQ=255)                                aTCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAAt                                                                                                         <  2:470159/140‑1 (MQ=255)                                       tAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTACTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTaa                                                                                                 <  2:1536279/140‑1 (MQ=255)                                        aGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTaa                                                                                                 <  2:729568/140‑1 (MQ=255)                                            aCCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                                                                             >  2:958612/1‑140 (MQ=255)                                               ccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                          >  1:1709759/1‑140 (MQ=255)                                                cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaa                                                                                         <  2:346933/140‑1 (MQ=255)                                                       aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTTCTTGCGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  2:1672889/137‑1 (MQ=255)                                                       aGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     >  1:1672889/1‑137 (MQ=255)                                                        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                 >  1:1228834/1‑140 (MQ=255)                                                          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                                                               >  2:796343/1‑140 (MQ=255)                                                            ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                                                             >  2:1583176/1‑140 (MQ=255)                                                             tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  2:1592313/1‑140 (MQ=255)                                                              gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                           <  1:836213/140‑1 (MQ=255)                                                              gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                           <  2:1116898/140‑1 (MQ=255)                                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                          >  1:459927/1‑140 (MQ=255)                                                               tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                          >  2:1400366/1‑140 (MQ=255)                                                                gACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                                                         >  1:5475/1‑140 (MQ=255)                                                                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  1:898101/1‑140 (MQ=255)                                                                     tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  2:317232/1‑140 (MQ=255)                                                                      gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                                                                   <  1:1187212/140‑1 (MQ=255)                                                                       cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                                                                   <  2:203308/140‑1 (MQ=255)                                                                       cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATAGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                                                                  >  2:223122/1‑140 (MQ=255)                                                                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg                                                          >  2:522433/1‑140 (MQ=255)                                                                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg                                                         <  2:1228834/140‑1 (MQ=255)                                                                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  2:623192/140‑1 (MQ=255)                                                                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  2:1629707/140‑1 (MQ=255)                                                                                        gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  1:1618595/140‑1 (MQ=255)                                                                                             cgcgCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGt                                            <  1:796343/140‑1 (MQ=255)                                                                                              gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGtt                                           <  1:522433/140‑1 (MQ=255)                                                                                              gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGtt                                           >  2:1773089/1‑140 (MQ=255)                                                                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTggg                                       >  2:354839/1‑140 (MQ=255)                                                                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAc                                     <  1:354839/140‑1 (MQ=255)                                                                                                       tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAg                                  <  1:1773089/140‑1 (MQ=255)                                                                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTgg                               >  1:1028257/1‑140 (MQ=255)                                                                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  1:976116/1‑140 (MQ=255)                                                                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  2:1080316/1‑140 (MQ=255)                                                                                                              ttAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  2:1059666/1‑140 (MQ=255)                                                                                                                aGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGa                         >  1:1162947/1‑140 (MQ=255)                                                                                                                    tCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  2:898101/140‑1 (MQ=255)                                                                                                                     ccTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTc                    >  2:727902/1‑140 (MQ=255)                                                                                                                         aCGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                >  2:387124/1‑140 (MQ=255)                                                                                                                                  ggggTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       <  2:459927/140‑1 (MQ=255)                                                                                                                                  ggggTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       >  2:171987/1‑140 (MQ=255)                                                                                                                                       tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:1028257/140‑1 (MQ=255)                                                                                                                                       tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:958612/140‑1 (MQ=255)                                                                                                                                       tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  >  1:1724776/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                       TGGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982537‑3982809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑