Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	94,844	A→G	L65L (CTA→CTG)	murF →	D‑alanyl‑D‑alanine‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	94,844	0	A	G	100.0%	334.9 / NA	92	L65L (CTA→CTG)	murF	D‑alanyl‑D‑alanine‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/45);  total (47/45)

CACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCG  >  NC_000913/94715‑94977                                                                                                                                  |                                                                                                                                      cACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGTGGCGCAGGCGCACTGCTGGTTAGcc                                                                                                                             <  2:511626/140‑1 (MQ=255) cACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGcc                                                                                                                             >  1:529112/1‑140 (MQ=255) cACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGcc                                                                                                                             <  1:970003/140‑1 (MQ=255) cACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGcc                                                                                                                             <  2:1537137/140‑1 (MQ=255) cACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGcc                                                                                                                             <  1:295005/140‑1 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  2:1054751/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  2:211552/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  1:351938/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  1:894010/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  1:832424/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  1:1438975/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  2:523989/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  1:765018/1‑140 (MQ=255)  aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCg                                                                                                                            >  1:533/1‑140 (MQ=255)    ccTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTc                                                                                                                          >  2:1069281/1‑140 (MQ=255)       tGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTTCTGGTTAGCCGTCCGc                                                                                                                       >  2:617370/1‑140 (MQ=255)       tGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGc                                                                                                                       >  1:1702583/1‑140 (MQ=255)        gATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCt                                                                                                                      >  2:344723/1‑140 (MQ=255)         aTGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTg                                                                                                                     >  1:1627374/1‑140 (MQ=255)         aTGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTg                                                                                                                     >  1:895665/1‑140 (MQ=255)             tGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACa                                                                                                                 <  1:1336174/140‑1 (MQ=255)             tGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACa                                                                                                                 >  2:777628/1‑140 (MQ=255)              gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACAt                                                                                                                >  2:1572651/1‑140 (MQ=255)              gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACAt                                                                                                                >  2:107602/1‑140 (MQ=255)              gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACAt                                                                                                                >  1:1594953/1‑140 (MQ=255)              gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACAt                                                                                                                >  2:819372/1‑140 (MQ=255)              gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACAt                                                                                                                <  1:1054751/140‑1 (MQ=255)                aaCCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCg                                                                                                              <  1:1445689/140‑1 (MQ=255)                  ccACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGAc                                                                                                            >  2:34922/1‑140 (MQ=255)                    aCTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCt                                                                                                          >  1:269190/1‑140 (MQ=255)                    aCTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCt                                                                                                          >  1:275385/1‑140 (MQ=255)                    aCTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCt                                                                                                          >  1:1600802/1‑140 (MQ=255)                      tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGc                                                                                                        <  1:1069281/140‑1 (MQ=255)                      tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGc                                                                                                        >  2:542106/1‑140 (MQ=255)                        aTACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCg                                                                                                      <  2:262845/140‑1 (MQ=255)                         tACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGCCCGCTGGACATCGACCTGCCGc                                                                                                     <  2:351938/140‑1 (MQ=255)                           cccGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAg                                                                                                   >  2:727614/1‑140 (MQ=255)                            ccGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGt                                                                                                  <  2:1627374/140‑1 (MQ=255)                              gAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGCCCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTa                                                                                                <  1:137876/140‑1 (MQ=255)                              gAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTa                                                                                                <  1:523989/140‑1 (MQ=255)                              gAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTa                                                                                                <  1:1603313/140‑1 (MQ=255)                               aaaaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTaa                                                                                               >  1:112313/1‑140 (MQ=255)                               aaaaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTaa                                                                                               <  1:819372/140‑1 (MQ=255)                               aaaaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTaa                                                                                               >  1:950648/1‑140 (MQ=255)                                aaaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTaa                                                                                               >  1:396420/1‑139 (MQ=255)                                aaaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTaa                                                                                               <  2:396420/139‑1 (MQ=255)                                aaaCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAAt                                                                                              <  1:1160482/140‑1 (MQ=255)                                  aCTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCg                                                                                            <  2:1702583/140‑1 (MQ=255)                                    tGACGCCGGGCTGCCTGTTTGTTTCGCGAAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGCCCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACCTCGACCTGCCGCAGTCAATCGTc                                                                                          <  2:575141/140‑1 (MQ=255)                                     gACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCa                                                                                         <  1:434465/140‑1 (MQ=255)                                      aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCaa                                                                                        >  2:63654/1‑140 (MQ=255)                                      aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCaa                                                                                        <  2:1465702/140‑1 (MQ=255)                                            ggCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATAc                                                                                  >  2:279271/1‑140 (MQ=255)                                                 ccTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTc                                                                             <  2:894010/140‑1 (MQ=255)                                                     tttgttGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGc                                                                         >  1:496837/1‑140 (MQ=255)                                                     tttgttGCCCTGAAAGGCGAACGTTTTGATGCCCACGATATTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGc                                                                         <  2:1277069/140‑1 (MQ=255)                                                         ttgCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGttt                                                                     <  1:777628/140‑1 (MQ=255)                                                          tgCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGTGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTg                                                                    <  1:1716066/140‑1 (MQ=255)                                                           gCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTgg                                                                   >  2:205250/1‑140 (MQ=255)                                                           gCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTgg                                                                   >  2:1692509/1‑140 (MQ=255)                                                               tGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGaa                                                               <  1:1502642/140‑1 (MQ=255)                                                                gAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAAc                                                              <  2:529112/140‑1 (MQ=255)                                                                 aaaGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACt                                                             <  1:1830917/140‑1 (MQ=255)                                                                   aGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTgg                                                           <  2:1438975/140‑1 (MQ=255)                                                                      cGAACGTTTTGATGCCCACGATTTTGTCGACCAGGCGGAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTg                                                        >  2:997071/1‑140 (MQ=255)                                                                      cGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCg                                                                        <  2:9729/124‑1 (MQ=255)                                                                      cGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCg                                                                        >  1:9729/1‑124 (MQ=255)                                                                       gAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGc                                                       >  2:219801/1‑140 (MQ=255)                                                                       gAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGc                                                       <  2:765018/140‑1 (MQ=255)                                                                             tttGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGt                                                 <  1:721637/140‑1 (MQ=255)                                                                                gATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCg                                              <  2:895665/140‑1 (MQ=255)                                                                                     ccACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGc                                         <  1:1692509/140‑1 (MQ=255)                                                                                      cACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCa                                        >  1:409841/1‑140 (MQ=255)                                                                                                  gACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcgcg                            <  2:1790395/140‑1 (MQ=255)                                                                                                  gACCAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcgcg                            <  2:1463061/140‑1 (MQ=255)                                                                                                     cAGGCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGACGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGt                         >  1:1699662/1‑140 (MQ=255)                                                                                                       ggCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTgg                       >  2:1492055/1‑140 (MQ=255)                                                                                                       ggCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTgg                       <  1:279271/140‑1 (MQ=255)                                                                                                        gCGAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGt                      <  1:205250/140‑1 (MQ=255)                                                                                                          gAAAGCTGGCGGCGCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTg                    <  1:1552240/140‑1 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcg                              <  2:119185/122‑1 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGgcg                              >  1:119185/1‑122 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGct                  <  1:135995/134‑1 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGct                  >  2:135995/1‑134 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACg            <  1:184734/140‑1 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACg            >  2:701833/1‑140 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACg            >  1:1124429/1‑140 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACg            <  2:1438442/140‑1 (MQ=255)                                                                                                                  gcggcgCAGGCGCACTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACg            >  2:25050/1‑140 (MQ=255)                                                                                                                        caggcgcaCTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGtcc      >  1:686329/1‑140 (MQ=255)                                                                                                                         aggcgcaCTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGtcct     <  1:755627/140‑1 (MQ=255)                                                                                                                         aggcgcaCTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGtcct     <  1:701833/140‑1 (MQ=255)                                                                                                                           gcgcaCTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGtcctcc   <  2:169110/140‑1 (MQ=255)                                                                                                                            cgcaCTGCTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCg  >  2:1140882/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                      CACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGTTTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCGACCTGCCGCAGTTAATCGTCAAGGATACGCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCG  >  NC_000913/94715‑94977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑