Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,163,830	C→T	L319L (CTG→TTG)	baeS →	sensory histidine kinase BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,163,830	0	C	T	100.0%	232.0 / NA	65	L319L (CTG→TTG)	baeS	sensory histidine kinase BaeS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/40);  total (25/40)

GCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTA  >  NC_000913/2163697‑2163958                                                                                                                                      |                                                                                                                                  gCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAg                                                                                                                             >  1:1231854/1‑140 (MQ=255)           gTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTTGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGGTTTGATCCCACTGTTGGAAGTGgcgggcgg                                                                                                                   >  2:48754/1‑140 (MQ=255)               cGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCa                                                                                                               <  1:675059/140‑1 (MQ=255)               cGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCa                                                                                                               >  1:478909/1‑140 (MQ=255)                  cTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCAttt                                                                                                            >  1:548268/1‑140 (MQ=255)                           ggagggTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGa                                                                                                  <  1:1476490/136‑1 (MQ=255)                           cGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGa                                                                                                  <  2:1109486/140‑1 (MQ=255)                             gAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGAtt                                                                                                >  1:1643627/1‑140 (MQ=255)                                gTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGc                                                                                             <  1:1413778/140‑1 (MQ=255)                                      acacTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCg                                                                                       <  2:1518293/140‑1 (MQ=255)                                           gACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCTCG‑AACGAATCGCCCGGCGGGGcc                                                                                  >  2:1675835/1‑140 (MQ=255)                                            aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCCTTTTGCG‑AACGAATTGCCAGTCGTgggcc                                                                                 >  2:1427165/1‑137 (MQ=255)                                            aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCCCTTCCCG‑AACGAATCGCCCGGCGTgggcg                                                                                 >  2:661805/1‑137 (MQ=255)                                            aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTTGCG‑AACGATTCGCCAGTCGTGGCCt                                                                                 >  1:1192355/1‑140 (MQ=255)                                            aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCTCG‑AACGATTCGCCCGTCGTGGccc                                                                                 >  1:1750597/1‑139 (MQ=255)                                            aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCt                                                                                 >  1:288762/1‑140 (MQ=255)                                                 aCTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGGCTTTCGCG‑AACGATTCGCCCGCCGTGGCCTGaaaa                                                                            >  2:118717/1‑139 (MQ=255)                                                 aCTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAAc                                                                            <  2:1589404/140‑1 (MQ=255)                                                      ttGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGGGCATTTTTCG‑GACGATTTGCCAGTCGTGGGCTGGAAAAGCaa                                                                       >  2:1512028/1‑140 (MQ=255)                                                      ttGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGGCTGAAACTGCaa                                                                       >  1:107566/1‑140 (MQ=255)                                                      ttGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCaa                                                                       >  1:359616/1‑140 (MQ=255)                                                      ttGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCaa                                                                       >  1:759574/1‑140 (MQ=255)                                                      ttGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGAATCGCCAGTCGTGGCCTGAAAATTCaa                                                                       >  1:1149760/1‑140 (MQ=255)                                                          cGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAAtttt                                                                   <  1:518591/140‑1 (MQ=255)                                                          cGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAAtttt                                                                   <  2:724156/140‑1 (MQ=255)                                                          cGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAAtttt                                                                   <  1:661805/140‑1 (MQ=255)                                                            aTCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTcc                                                                 <  1:1132823/140‑1 (MQ=255)                                                               tcCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTg                                                              <  2:1643627/140‑1 (MQ=255)                                                               tcCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTg                                                              <  1:420754/140‑1 (MQ=255)                                                               gagacaggTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTg                                                              <  1:1250482/133‑1 (MQ=255)                                                                ccATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGc                                                             <  1:1675835/140‑1 (MQ=255)                                                                  aTCAGTTGTTGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGGTTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCa                                                           <  1:48754/140‑1 (MQ=255)                                                                  aTCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCa                                                           <  2:1231854/140‑1 (MQ=255)                                                                  aTCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCa                                                           <  2:759574/140‑1 (MQ=255)                                                                  aTCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCa                                                           <  1:1260981/140‑1 (MQ=255)                                                                   tCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGGGGGGGCTTTCTCG‑AACGATTTGCCACTCGTTGCCTGTAACTCCAATTTTTCCTccccg                                                          >  1:1361130/1‑138 (MQ=255)                                                                    cAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGa                                                         <  2:548268/140‑1 (MQ=255)                                                                    cAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGa                                                         <  2:1332973/140‑1 (MQ=255)                                                                       ttGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGGGGCGCCCTTTTCGCAAGAATTCCCCCG‑CGTGGGCTGAAACTGCAATTTTTCCTCCCAGACAg                                                      >  2:1111339/1‑140 (MQ=255)                                                                       ttGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑GACGATTCGCCCGTCGTGGCCTGAAAATGCAATTTTTCCTTCCCGACaa                                                      >  2:1132823/1‑139 (MQ=255)                                                                        tGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGt                                                     <  1:994019/140‑1 (MQ=255)                                                                         gTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTTGCG‑AACGATTCGCCCGTCGTGGGCCGAAACTGCAATTTTTCCTTCCCGACAgga                                                    >  2:1260981/1‑138 (MQ=255)                                                                         gTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTa                                                    <  1:118717/140‑1 (MQ=255)                                                                                 cTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGGGCATTTTGCG‑AACGATTCGCCCGTCGTGGCCTGAAAATGCAAATTTTCCTGCCCCACAGAAtctgtctc                                            >  2:1250482/1‑133 (MQ=255)                                                                                              tctcGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCCTTTCGCG‑AACCAATTTCCAGTCGTGGGCCGAAACTGCAATTTTTCCCCCCAGACAGTATTACCGTATTTTGCGAAcgcg                               >  2:1564897/1‑140 (MQ=255)                                                                                               ctcGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGa                              <  1:1688962/140‑1 (MQ=255)                                                                                               ctcGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGa                              <  1:1512028/140‑1 (MQ=255)                                                                                               ctcGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGa                              <  1:932352/140‑1 (MQ=255)                                                                                               ctcGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGa                              <  1:569996/140‑1 (MQ=255)                                                                                                 cGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGAcc                            <  1:507578/140‑1 (MQ=255)                                                                                                 cGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGAcc                            <  2:288762/140‑1 (MQ=255)                                                                                                       tCAAAAAGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTaa                      <  1:960887/140‑1 (MQ=255)                                                                                                         aaaaaGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATg                    <  1:109620/140‑1 (MQ=255)                                                                                                          aaaaGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGc                   <  1:152502/140‑1 (MQ=255)                                                                                                            aaGCACCGGTAGATTTGAT‑CCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGt                <  1:1817346/140‑1 (MQ=255)                                                                                                             aGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGt                <  1:10769/140‑1 (MQ=255)                                                                                                             aGCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGt                <  2:243694/140‑1 (MQ=255)                                                                                                              gCACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGGGCCTTTTGCG‑AACAATTCCCCCGGCGTGGGCTGAAAATGCAATTTTTCCTGCCCGACAGTATTACCGTATTTTGCGAACGCGACCGCTTAATGCAGtt               >  2:152502/1‑140 (MQ=255)                                                                                                               cACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTa              <  1:1560766/140‑1 (MQ=255)                                                                                                               cACCGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTa              <  2:478909/140‑1 (MQ=255)                                                                                                                 ccGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTTTCG‑AACGAATTGCCCGGCGGGGGCCGGAAATGCAATTTTTCCTCCCCGACAGAAATAACGTATTTTGCGGTCGCGGCCGGTTTATGCAGGTAtt            >  2:10769/1‑140 (MQ=255)                                                                                                                 ccGGTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTAtt            <  1:183205/140‑1 (MQ=255)                                                                                                                   ggTAGATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCa          <  1:1039512/140‑1 (MQ=255)                                                                                                                       gATTTGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCACCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCTATaa      <  2:731935/140‑1 (MQ=255)                                                                                                                           tGATCCCACTGTTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTa  <  1:648793/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  GCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTA  >  NC_000913/2163697‑2163958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑