BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,664	T→C	T169A (ACA→GCA)	yfdE ←	acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,664	0	T	C	100.0%	568.3 / NA	154	T169A (ACA→GCA)	yfdE	acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (77/77); total (77/77)

Only 100 of 155 total aligned reads displayed.

TGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATG  >  NC_000913/2488528‑2488800

tGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCAcc                                                                                                                                       >  2:1190145/1‑140 (MQ=255)

      aGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAAcgcg                                                                                                                                 >  1:621738/1‑140 (MQ=255)

      aGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAAcgcg                                                                                                                                 >  2:1533831/1‑140 (MQ=255)

      aGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAAcgcg                                                                                                                                 >  1:1418885/1‑140 (MQ=255)

        aaaaCTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCa                                                                                                                               <  1:1670496/140‑1 (MQ=255)

        aaaaCTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGCGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCa                                                                                                                               <  2:638434/140‑1 (MQ=255)

            cTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTgg                                                                                                                           >  2:762787/1‑140 (MQ=255)

              cAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCg                                                                                                                         <  2:1418885/140‑1 (MQ=255)

              cAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCg                                                                                                                         <  1:762787/140‑1 (MQ=255)

               aGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCgg                                                                                                                        >  2:600320/1‑140 (MQ=255)

                    ggCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCAt                                                                                                                   >  1:1684562/1‑140 (MQ=255)

                     gCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCTCTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATc                                                                                                                  <  1:285075/140‑1 (MQ=255)

                     gCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGGATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATc                                                                                                                  <  2:1743575/140‑1 (MQ=255)

                         cAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGa                                                                                                              <  1:916245/140‑1 (MQ=255)

                          aaaCATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGat                                                                                                             <  1:108255/140‑1 (MQ=255)

                               tCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCa                                                                                                                    <  1:1383775/128‑1 (MQ=255)

                               tCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCa                                                                                                                    >  2:1383775/1‑128 (MQ=255)

                                cGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTg                                                                                                       >  2:659777/1‑140 (MQ=255)

                                cGCTATATCGACATACGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTg                                                                                                       >  2:689925/1‑140 (MQ=255)

                                    atatCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTc                                                                                                   <  2:170773/140‑1 (MQ=255)

                                    atatCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTc                                                                                                   <  1:175769/140‑1 (MQ=255)

                                    atatCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTc                                                                                                   <  2:1199102/140‑1 (MQ=255)

                                         gACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCcatca                                                                                              <  1:741395/140‑1 (MQ=255)

                                          aCATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCcatcat                                                                                             >  1:1409910/1‑140 (MQ=255)

                                          aCATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCcatcat                                                                                             >  1:22241/1‑140 (MQ=255)

                                             tGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTAt                                                                                          >  2:422592/1‑140 (MQ=255)

                                               cgcCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTc                                                                                                   >  1:673405/1‑129 (MQ=255)

                                               cgcCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATcc                                                                                        >  1:1017189/1‑140 (MQ=255)

                                               cgcCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATcc                                                                                        <  1:1810985/140‑1 (MQ=255)

                                               cgcCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATcc                                                                                        >  2:879068/1‑140 (MQ=255)

                                                   ccTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCt                                                                                    <  1:139286/140‑1 (MQ=255)

                                                     tctctGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTc                                                                                                   >  1:1177030/1‑123 (MQ=255)

                                                      ctctGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCAt                                                                                 <  2:798820/140‑1 (MQ=255)

                                                       tctGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCAtt                                                                                >  1:90662/1‑140 (MQ=255)

                                                         tGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGc                                                                              <  1:159469/140‑1 (MQ=255)

                                                         tGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGc                                                                              <  2:914524/140‑1 (MQ=255)

                                                              ctTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGGCACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGaa                                                                         <  1:1064815/140‑1 (MQ=255)

                                                              ctTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGaa                                                                         >  2:1707140/1‑140 (MQ=255)

                                                              ctTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGaa                                                                         <  1:809476/140‑1 (MQ=255)

                                                                tttCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCGAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAatg                                                                       >  2:1806267/1‑140 (MQ=255)

                                                                 ttCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCt                                                                                    >  1:160212/1‑126 (MQ=255)

                                                                 ttCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCt                                                                                    <  2:160212/126‑1 (MQ=255)

                                                                 ttCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAatga                                                                      <  1:81782/140‑1 (MQ=255)

                                                                 ttCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAatga                                                                      >  2:1228590/1‑140 (MQ=255)

                                                                   cgcgGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAatgatg                                                                    <  1:1478953/140‑1 (MQ=255)

                                                                     cgGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGt                                                                  >  1:891744/1‑140 (MQ=255)

                                                                           tAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTa                                                            <  2:1684562/140‑1 (MQ=255)

                                                                                  cactcactATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGa                                                     >  2:378623/1‑140 (MQ=255)

                                                                                  cactcactATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGa                                                     >  2:299806/1‑140 (MQ=255)

                                                                                  cactcactATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGa                                                     >  2:1470645/1‑140 (MQ=255)

                                                                                      cactATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCAt                                                 >  1:1270247/1‑140 (MQ=255)

                                                                                       actATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATc                                                >  1:35889/1‑140 (MQ=255)

                                                                                              cGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAgg                                                          >  1:1284965/1‑123 (MQ=255)

                                                                                              cGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAgg                                                          <  2:1284965/123‑1 (MQ=255)

                                                                                              cGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGc                                         >  1:1395732/1‑140 (MQ=255)

                                                                                                cTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCgg                                       <  2:1216001/140‑1 (MQ=255)

                                                                                                 tGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGa                                      >  2:442219/1‑140 (MQ=255)

                                                                                                 tGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGa                                      >  1:953573/1‑140 (MQ=255)

                                                                                                 tGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGa                                      <  2:427637/140‑1 (MQ=255)

                                                                                                 tGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGa                                      >  2:876491/1‑140 (MQ=255)

                                                                                                 tGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGa                                      <  1:1202001/140‑1 (MQ=255)

                                                                                                     taaataGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCgg                                  <  1:422592/140‑1 (MQ=255)

                                                                                                      aaataGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGt                                 <  2:22241/140‑1 (MQ=255)

                                                                                                      aaataGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGt                                 <  2:319681/140‑1 (MQ=255)

                                                                                                       aataGACACCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTa                                <  2:1611356/140‑1 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTa                                           >  1:84755/1‑123 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTa                                           <  2:84755/123‑1 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCg                          >  2:1785317/1‑140 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCg                          >  1:96187/1‑140 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCg                          >  2:790414/1‑140 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCg                          >  1:1826812/1‑140 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCg                          >  2:1033078/1‑140 (MQ=255)

                                                                                                             cacCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCg                          >  1:927839/1‑140 (MQ=255)

                                                                                                              acCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGa                         >  2:939460/1‑140 (MQ=255)

                                                                                                              acCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGa                         <  1:1173060/140‑1 (MQ=255)

                                                                                                              acCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGa                         <  1:1785317/140‑1 (MQ=255)

                                                                                                              acCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGa                         <  2:1270247/140‑1 (MQ=255)

                                                                                                              acCGCCGCATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGa                         >  2:213724/1‑140 (MQ=255)

                                                                                                                cgccgcATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGaaa                       >  2:532849/1‑140 (MQ=255)

                                                                                                                cgccgcATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCACCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGaaa                       >  1:1624729/1‑140 (MQ=255)

                                                                                                                  ccgcATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAAcc                     >  1:1753190/1‑140 (MQ=255)

                                                                                                                     cATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGa                  <  2:1396015/140‑1 (MQ=255)

                                                                                                                     cATAGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGa                  >  1:371188/1‑140 (MQ=255)

                                                                                                                        aGATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGa               <  2:1395732/140‑1 (MQ=255)

                                                                                                                         gATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGAt              <  1:1228590/140‑1 (MQ=255)

                                                                                                                         gATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGAt              <  1:1470645/140‑1 (MQ=255)

                                                                                                                         gATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGAt              <  2:1753190/140‑1 (MQ=255)

                                                                                                                         gATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGAt              <  2:76110/140‑1 (MQ=255)

                                                                                                                         gATCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGAt              <  1:1783809/140‑1 (MQ=255)

                                                                                                                          aTCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATg             <  1:939460/140‑1 (MQ=255)

                                                                                                                          aTCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATg             <  2:1385584/140‑1 (MQ=255)

                                                                                                                          aTCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATg             <  2:1093850/140‑1 (MQ=255)

                                                                                                                          aTCCGCAAGAGATGCACCAACGCGCACTGGCGCAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATg             >  2:1436937/1‑140 (MQ=255)

                                                                                                                           tCCGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGa            <  1:659777/140‑1 (MQ=255)

                                                                                                                            ccGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGaa           <  1:479558/140‑1 (MQ=255)

                                                                                                                             cGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAg          >  1:303429/1‑140 (MQ=255)

                                                                                                                             cGCAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAg          <  2:45106/140‑1 (MQ=255)

                                                                                                                               cAAGAGATGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCa        <  1:399062/140‑1 (MQ=255)

                                                                                                                                    gaTGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATg             <  2:1364691/130‑1 (MQ=255)

                                                                                                                                     aTGCACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATg  >  1:1015214/1‑140 (MQ=255)

TGCTCCAGAAAACTCAGCGTGGCATCAAACATCGCTATATCGACATGCGCCCCTCTCTGGCTCTTTTCGCGGCCATAAAGTGCACTCACTATTCCGCTGAATAAATAGACACCGCCGCATAGATCCGCAAGAGATGTACCAACGCGCACTGGCGGAGCATCAGGATATCCTGTTTCCATCATTATCCCGCTCATTGCCTGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATG  >  NC_000913/2488528‑2488800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`