Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	166,980	A→G	T751A (ACG→GCG)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	166,980	0	A	G	100.0%	341.2 / NA	93	T751A (ACG→GCG)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/45);  total (48/45)

CGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAG  >  NC_000913/166844‑167114                                                                                                                                         |                                                                                                                                       cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGgcgc                                                                                                                                     >  1:317120/1‑140 (MQ=255) cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGgcgc                                                                                                                                     >  2:218031/1‑140 (MQ=255) cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGgcgc                                                                                                                                     >  1:1251166/1‑140 (MQ=255)  gTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGcc                                                                                                                                    <  2:151468/140‑1 (MQ=255)    aGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCaa                                                                                                                                  <  2:1478289/140‑1 (MQ=255)    aGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCAGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCaa                                                                                                                                  <  1:1386516/140‑1 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                <  1:218031/140‑1 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                <  1:338203/140‑1 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                >  1:364234/1‑140 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                >  1:385192/1‑140 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                >  2:1004332/1‑140 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                >  2:163201/1‑140 (MQ=255)      aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                >  2:831225/1‑140 (MQ=255)        aCCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcgcc                                                                                                                              >  2:173943/1‑140 (MQ=255)         ccTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcgccg                                                                                                                             >  2:1710027/1‑140 (MQ=255)           tGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCt                                                                                                                           <  2:1374772/140‑1 (MQ=255)           tGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCt                                                                                                                           <  1:831225/140‑1 (MQ=255)           tGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCt                                                                                                                           >  2:914278/1‑140 (MQ=255)            ggTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTg                                                                                                                          <  2:317120/140‑1 (MQ=255)            ggTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTg                                                                                                                          >  1:1025134/1‑140 (MQ=255)             gTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGa                                                                                                                         >  1:1579717/1‑140 (MQ=255)              tttGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGaa                                                                                                                        >  1:723367/1‑140 (MQ=255)               ttGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAAt                                                                                                                       >  1:13179/1‑140 (MQ=255)                tGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCAATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                <  2:1148452/130‑1 (MQ=255)                tGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCAATTTATCAGCGTTATCTGGCTAACCAGGCGCCAAcg                                                                                                                                >  1:1148452/1‑130 (MQ=255)                      cATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACTAGGCGCCAACGCCGCTGAATCTTGTTc                                                                                                                <  1:1600648/140‑1 (MQ=255)                      cATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTc                                                                                                                <  2:385192/140‑1 (MQ=255)                        ttGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCg                                                                                                              >  2:48335/1‑140 (MQ=255)                           aCGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCa                                                                                                           <  1:1789084/140‑1 (MQ=255)                           aCGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCa                                                                                                           <  2:768840/140‑1 (MQ=255)                             ggCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCaga                                                                                                         >  2:721736/1‑140 (MQ=255)                              gcagcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCagaa                                                                                                        <  1:163201/140‑1 (MQ=255)                              gcagcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCagaa                                                                                                        <  2:758933/140‑1 (MQ=255)                                agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                                                      <  2:1775795/140‑1 (MQ=255)                                agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                                                      <  2:1579717/140‑1 (MQ=255)                                agcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                                                      <  1:161257/140‑1 (MQ=255)                                   aCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGatat                                                                                                   >  1:399133/1‑140 (MQ=255)                                    cGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATAtt                                                                                                  <  2:1251166/140‑1 (MQ=255)                                      gTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGc                                                                                                <  1:1710027/140‑1 (MQ=255)                                       tGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCa                                                                                               >  1:72023/1‑140 (MQ=255)                                           cATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                                                           <  2:162869/140‑1 (MQ=255)                                           cATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                                                           <  2:1746202/140‑1 (MQ=255)                                           cATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                                                           >  2:1399909/1‑140 (MQ=255)                                           cATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                                                           <  1:295185/140‑1 (MQ=255)                                                 cTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCg                                                                                     <  2:72023/140‑1 (MQ=255)                                                 cTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCg                                                                                     <  2:399133/140‑1 (MQ=255)                                                 cTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCg                                                                                     <  1:48335/140‑1 (MQ=255)                                                    ggTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCATTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTgg                                                                                  <  1:1612364/140‑1 (MQ=255)                                                     gTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGa                                                                                 >  2:467622/1‑140 (MQ=255)                                                     gTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGa                                                                                 >  1:500730/1‑140 (MQ=255)                                                     gTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGa                                                                                 >  1:387316/1‑140 (MQ=255)                                                         gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCGGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                                                           >  2:1654910/1‑126 (MQ=255)                                                         gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCGGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                                                           <  1:1654910/126‑1 (MQ=255)                                                         gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTac                                                                             >  2:940543/1‑140 (MQ=255)                                                         gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTac                                                                             >  2:749395/1‑140 (MQ=255)                                                               aTAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGc                                                                       >  1:95080/1‑140 (MQ=255)                                                                tAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCa                                                                      <  2:1431049/140‑1 (MQ=255)                                                                       cAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACtttgt                                                               >  2:661449/1‑140 (MQ=255)                                                                         gCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACgg                                                                        >  2:769508/1‑129 (MQ=255)                                                                         gCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACgg                                                                        <  1:769508/129‑1 (MQ=255)                                                                          ccGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACtttgtttg                                                            <  2:536907/140‑1 (MQ=255)                                                                                 aaCTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGt                                                     <  2:924104/140‑1 (MQ=255)                                                                                  aCTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTg                                                    <  2:1587906/140‑1 (MQ=255)                                                                                   cTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTgg                                                   <  2:526345/140‑1 (MQ=255)                                                                                   cTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTgg                                                   <  1:1004332/140‑1 (MQ=255)                                                                                      tATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCAt                                                <  2:1025134/140‑1 (MQ=255)                                                                                        tGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGc                                              >  1:492534/1‑140 (MQ=255)                                                                                         ggTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCg                                             >  1:1523313/1‑140 (MQ=255)                                                                                           tGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTa                                           >  1:831471/1‑140 (MQ=255)                                                                                           tGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTa                                           >  1:887161/1‑140 (MQ=255)                                                                                           tGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTATTTGCAGCGGTGGCATGCGTa                                           >  2:1218896/1‑140 (MQ=255)                                                                                           tGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCCGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTa                                           >  2:878974/1‑140 (MQ=255)                                                                                            gCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATAGGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTgg                                                   >  2:1138059/1‑131 (MQ=255)                                                                                            gCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATAGGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTgg                                                   <  1:1138059/131‑1 (MQ=255)                                                                                             ccAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATTGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATc                                         >  2:228714/1‑140 (MQ=255)                                                                                             ccAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATc                                         >  2:1701118/1‑140 (MQ=255)                                                                                             ccAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATc                                         >  1:1195493/1‑140 (MQ=255)                                                                                             ccAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAAGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGGTATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATc                                         >  2:1617194/1‑140 (MQ=255)                                                                                              cAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCt                                        >  2:472149/1‑140 (MQ=255)                                                                                              cAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCt                                        >  1:1281488/1‑140 (MQ=255)                                                                                              cAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCt                                        >  1:1532276/1‑140 (MQ=255)                                                                                                gCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATGTTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTg                                      >  1:1576000/1‑140 (MQ=255)                                                                                                 cGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGc                                     <  1:749395/140‑1 (MQ=255)                                                                                                     gCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGt                                 >  2:639672/1‑140 (MQ=255)                                                                                                     gCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGt                                 >  2:1011330/1‑140 (MQ=255)                                                                                                     gCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGt                                 >  2:1471551/1‑140 (MQ=255)                                                                                                      cAATGTCGATTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTc                                <  1:1800192/140‑1 (MQ=255)                                                                                                              aTTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAg                        >  2:989633/1‑140 (MQ=255)                                                                                                              aTTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAg                        <  1:1609732/140‑1 (MQ=255)                                                                                                              aTTTATCAGCGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAg                        >  1:1189552/1‑140 (MQ=255)                                                                                                                       cGTTATCTGGCTAACCAGGCGCCAACGCTGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCa               >  1:1287326/1‑140 (MQ=255)                                                                                                                       cGTTATCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCa               <  2:723367/140‑1 (MQ=255)                                                                                                                           aTCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCg           >  2:49393/1‑140 (MQ=255)                                                                                                                            tCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGc          <  2:1523313/140‑1 (MQ=255)                                                                                                                            tCTGGCTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGc          <  2:683986/140‑1 (MQ=255)                                                                                                                                 cTAACCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGc     <  1:689938/140‑1 (MQ=255)                                                                                                                                    aCCAGGCGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCcag  <  2:492534/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTCTGGACCAGCGATCCGCAATCGCTGTGCCAG  >  NC_000913/166844‑167114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑