Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,470,718	C→A	E204D (GAG→GAT)	tufA ←	translation elongation factor Tu 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,470,718	0	C	A	100.0%	222.8 / NA	97	E204D (GAG→GAT)	tufA	translation elongation factor Tu 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (51/46);  total (51/46)

ATTTCAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGCTCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTACTGA  >  NC_000913/3470596‑3470853                                                                                                                           |                                                                                                                                        aTTTCAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAAtata                                                                                                                        <  2:171153/140‑1 (MQ=17)  tttCAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATaa                                                                                                                       <  2:271377/140‑1 (MQ=18)  tttCAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATaa                                                                                                                       <  2:646716/140‑1 (MQ=18)  tttCAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGGTCCGGAATATaa                                                                                                                       >  1:1282819/1‑140 (MQ=17)     cAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGaa                                                                                                                    <  1:31442/140‑1 (MQ=18)         ttcttcACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGTAATATAAGAATCCa                                                                                                                <  2:1532235/140‑1 (MQ=17)         ttcttcACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCa                                                                                                                >  2:481734/1‑140 (MQ=17)              cACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAag                                                                                                           <  1:1215892/140‑1 (MQ=18)              cACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAag                                                                                                           >  2:912100/1‑140 (MQ=17)               aCCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagc                                                                                                          <  1:661402/140‑1 (MQ=18)                   aCTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      <  2:1426153/140‑1 (MQ=18)                   aCTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      <  1:730960/140‑1 (MQ=18)                   aCTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      <  1:735226/140‑1 (MQ=18)                   aCTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      <  1:957200/140‑1 (MQ=18)                   aCTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      <  2:1396265/140‑1 (MQ=18)                          tgatACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTcc                                                                                               >  2:964532/1‑140 (MQ=17)                           gatACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCa                                                                                              >  1:1503568/1‑140 (MQ=17)                           gatACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCa                                                                                              >  1:1235542/1‑140 (MQ=17)                           gatACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCa                                                                                              >  1:48070/1‑140 (MQ=17)                              aCCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      <  2:106088/129‑1 (MQ=18)                              aCCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAagccagc                                                                                                      >  1:106088/1‑129 (MQ=17)                              aCCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGa                                                                                           >  1:1551767/1‑140 (MQ=17)                              aCCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGa                                                                                           >  2:1810808/1‑140 (MQ=17)                               ccGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGAt                                                                                          >  1:856605/1‑140 (MQ=17)                               ccGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGAt                                                                                          >  2:799902/1‑140 (MQ=17)                                  cgTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGAtttt                                                                                       >  1:920068/1‑140 (MQ=17)                                  cgTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGAttt                                                                                        >  1:34391/1‑139 (MQ=17)                                  cgTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGAttt                                                                                        <  2:34391/139‑1 (MQ=18)                                   gTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTc                                                                                      <  1:511227/140‑1 (MQ=18)                                    ttCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCg                                                                                     >  1:1340086/1‑140 (MQ=17)                                    ttCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCg                                                                                     >  2:929349/1‑140 (MQ=17)                                      cTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCt                                                                                   <  2:689270/140‑1 (MQ=18)                                         cacGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTcc                                                                                >  2:1133635/1‑140 (MQ=17)                                              ccGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCActc                                                                           >  2:1038516/1‑140 (MQ=17)                                               cGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCActct                                                                          >  1:399897/1‑140 (MQ=17)                                                 gTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGc                                                                        >  2:667498/1‑140 (MQ=17)                                                 gTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGc                                                                        >  1:689137/1‑140 (MQ=17)                                                        cGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCt                                                                 >  1:996504/1‑140 (MQ=17)                                                           tACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTcc                                                              <  1:1314166/140‑1 (MQ=17)                                                            aCCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCa                                                             >  1:1722956/1‑140 (MQ=17)                                                            aCCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCa                                                             >  2:1182007/1‑140 (MQ=17)                                                            aCCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCa                                                             >  1:1596865/1‑140 (MQ=17)                                                              cACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAgc                                                           >  2:707232/1‑140 (MQ=17)                                                              cACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAgc                                                           <  2:1308800/140‑1 (MQ=17)                                                                cGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAgcgc                                                         >  1:276224/1‑140 (MQ=17)                                                                cGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGACAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAgcgc                                                         >  2:1383185/1‑140 (MQ=17)                                                                 gACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCt                                                        <  1:1281713/140‑1 (MQ=17)                                                                  aCCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCtt                                                       >  1:1779107/1‑140 (MQ=17)                                                                   ccGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCttt                                                      <  1:1230687/140‑1 (MQ=17)                                                                   ccGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCttt                                                      <  1:799902/140‑1 (MQ=17)                                                                   ccGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCttt                                                      <  2:1551767/140‑1 (MQ=17)                                                                   ccGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCttt                                                      >  1:552330/1‑140 (MQ=17)                                                                     ggAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCa                                                    <  2:1503568/140‑1 (MQ=17)                                                                     ggAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCa                                                    >  2:1177012/1‑140 (MQ=17)                                                                        gaTGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCagag                                                 <  2:1722956/140‑1 (MQ=17)                                                                        gaTGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCagag                                                 <  2:996504/140‑1 (MQ=17)                                                                                aTACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCa                                         <  1:1133635/140‑1 (MQ=17)                                                                                aTACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCa                                         <  2:1282819/140‑1 (MQ=17)                                                                                 tACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGGAGAACCac                                        >  2:729539/1‑140 (MQ=17)                                                                                 tACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCac                                        >  2:419935/1‑140 (MQ=17)                                                                                 tACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCac                                        >  2:180426/1‑140 (MQ=17)                                                                                 tACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCac                                        >  2:656587/1‑140 (MQ=17)                                                                                  aCGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGGAGAACCacg                                       <  1:729539/140‑1 (MQ=12)                                                                                    gTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCttt                                                      >  1:403304/1‑123 (MQ=17)                                                                                    gTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCttt                                                      <  2:403304/123‑1 (MQ=17)                                                                                    gTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCacgaa                                     <  1:1230402/140‑1 (MQ=17)                                                                                    gTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCacgaa                                     <  1:685475/140‑1 (MQ=17)                                                                                      cTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCacgaacg                                   >  1:482622/1‑140 (MQ=17)                                                                                       ttCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCacgaacga                                  >  1:1367544/1‑140 (MQ=17)                                                                                       ttCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCacgaacga                                  >  1:1695687/1‑140 (MQ=17)                                                                                       ttCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCacgaacga                                  >  2:1095791/1‑140 (MQ=17)                                                                                        tCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGAt                                 <  1:1038516/140‑1 (MQ=17)                                                                                        tCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGAt                                 <  1:1165393/140‑1 (MQ=17)                                                                                        tCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGAt                                 <  1:1544226/140‑1 (MQ=17)                                                                                        tCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGACAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGAt                                 <  1:1383185/140‑1 (MQ=17)                                                                                         cGATCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATc                                >  2:445286/1‑140 (MQ=17)                                                                                           aTCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCgg                              >  2:611760/1‑140 (MQ=17)                                                                                           aTCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCgg                              >  2:535067/1‑140 (MQ=17)                                                                                            tCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGa                             <  1:611760/140‑1 (MQ=17)                                                                                            tCGGCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGa                             <  2:276224/140‑1 (MQ=17)                                                                                              ggCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAgt                           >  1:598742/1‑140 (MQ=17)                                                                                              ggCAGCAGGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAgg                           >  1:47634/1‑139 (MQ=17)                                                                                                   cagGAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTgtcgt                      >  1:452605/1‑140 (MQ=17)                                                                                                     ggAACGGCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGtcgtcg                    <  2:482622/140‑1 (MQ=17)                                                                                                           gCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCggg              <  1:419935/140‑1 (MQ=17)                                                                                                           gCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCggg              <  2:1187568/140‑1 (MQ=17)                                                                                                           gCTTGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCggg              <  1:649004/140‑1 (MQ=17)                                                                                                             ttGTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGGGTCCTCCCCCGGGaa            >  2:1312861/1‑140 (MQ=255)                                                                                                               gTCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGt          <  1:438449/140‑1 (MQ=17)                                                                                                                tCAATCGCACGATGTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGGGTCGTCGCCCGGGAAGTc         >  2:1226089/1‑140 (MQ=12)                                                                                                                tCAATCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTc         >  1:815778/1‑140 (MQ=18)                                                                                                                  aaTCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGt       <  2:1779107/140‑1 (MQ=17)                                                                                                                  aaTCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGt       <  2:815778/140‑1 (MQ=17)                                                                                                                   aTCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTa      <  2:1695687/140‑1 (MQ=17)                                                                                                                    tCGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTAc     <  1:1177012/140‑1 (MQ=17)                                                                                                                     cGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTACt    >  1:766242/1‑140 (MQ=18)                                                                                                                     cGCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTACt    >  1:1446288/1‑140 (MQ=17)                                                                                                                      gCACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTACTg   >  1:948710/1‑140 (MQ=18)                                                                                                                       cACGATCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTACTga  >  1:1378800/1‑140 (MQ=17)                                                                                                                           |                                                                                                                                        ATTTCAACTTCTTCACCAACTTTGATGATACCGCGTTCTACACGACCGGTAACAACGGTACCACGACCGGAGATGGAGAATACGTCTTCGATCGGCAGCAGGAACGGCTTGTCAATCGCACGCTCTGGTTCCGGAATATAAGAATCCAGGAAGCCAGCCAGTTCCAGGATTTTCGCTTCCCACTCTGCGTCGCCTTCCAGCGCTTTCAGAGCAGAACCACGAACGATCGGAGTGTCGTCGCCCGGGAAGTCGTACTGA  >  NC_000913/3470596‑3470853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑