Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,996,611	C→A	H109N (CAT→AAT)	xerC →	site‑specific tyrosine recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,996,611	0	C	A	100.0%	281.7 / NA	79	H109N (CAT→AAT)	xerC	site‑specific tyrosine recombinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/52);  total (27/52)

CCGTAAAGGGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTACGGCGCGGGTCTGCG  >  NC_000913/3996478‑3996729                                                                                                                                      |                                                                                                                       ccGTAAAGGGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGc                                                                                                                  >  2:672312/1‑140 (MQ=255)    tAAAGGGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGa                                                                                                               <  1:1473541/140‑1 (MQ=255)          gCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACa                                                                                                         <  2:275046/140‑1 (MQ=255)          gCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACa                                                                                                         <  2:7984/140‑1 (MQ=255)            tGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtc                                                                                                       >  1:148884/1‑140 (MQ=255)              ggAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcga                                                                                                     >  1:1411630/1‑140 (MQ=255)               gAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgac                                                                                                    <  1:1738208/140‑1 (MQ=255)                  cagcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtc                                                                                                 <  2:165440/140‑1 (MQ=255)                  cagcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtc                                                                                                 <  1:18028/140‑1 (MQ=255)                  cagcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtc                                                                                                 <  1:1433256/140‑1 (MQ=255)                   agcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcg                                                                                                <  2:1642596/140‑1 (MQ=255)                   agcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcg                                                                                                <  2:1513659/140‑1 (MQ=255)                    gcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcga                                                                                               >  2:25274/1‑140 (MQ=255)                    gcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcga                                                                                               >  2:838229/1‑140 (MQ=255)                    gcaAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcga                                                                                               <  1:25274/140‑1 (MQ=255)                            ggCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGa                                                                                       >  2:760243/1‑140 (MQ=255)                             gCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGaa                                                                                      >  2:1421555/1‑140 (MQ=255)                             gCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGaa                                                                                      >  1:95188/1‑140 (MQ=255)                               gTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATc                                                                                    <  2:1232803/140‑1 (MQ=255)                               gTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATc                                                                                    >  1:999962/1‑140 (MQ=255)                                 tACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCgg                                                                                  <  1:984723/140‑1 (MQ=255)                                 tACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCgg                                                                                  <  1:1701710/140‑1 (MQ=255)                                  aCGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcga                                                                                               >  2:837786/1‑126 (MQ=255)                                  aCGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACAtcgacgtcga                                                                                               <  1:837786/126‑1 (MQ=255)                                  aCGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgc                                                                                 >  1:1265652/1‑140 (MQ=255)                                  aCGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgc                                                                                 >  2:723782/1‑140 (MQ=255)                                   cGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgct                                                                                >  2:565050/1‑140 (MQ=255)                                   cGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgct                                                                                >  1:1112786/1‑140 (MQ=255)                                    ggCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctg                                                                               <  1:1213333/140‑1 (MQ=255)                                    ggCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctg                                                                               <  2:897426/140‑1 (MQ=255)                                    ggCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctg                                                                               <  2:151373/140‑1 (MQ=255)                                    ggCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTAAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctg                                                                               <  2:888087/140‑1 (MQ=255)                                      cTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctgct                                                                             <  2:1401120/140‑1 (MQ=255)                                      cTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctgct                                                                             <  2:823853/140‑1 (MQ=255)                                      cTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctgct                                                                             <  2:1112786/140‑1 (MQ=255)                                      cTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGgctgct                                                                             <  2:155004/140‑1 (MQ=255)                                           tGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGata                                                                        >  1:758667/1‑140 (MQ=255)                                           tGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGata                                                                        <  1:672312/140‑1 (MQ=255)                                            gcgcTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGatat                                                                       <  1:898246/140‑1 (MQ=255)                                              gcTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTg                                                                     <  2:451578/140‑1 (MQ=255)                                                tACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGat                                                                   <  2:1764585/140‑1 (MQ=255)                                                 aCGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGata                                                                  <  2:727742/140‑1 (MQ=255)                                                  cGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGatat                                                                 <  1:790775/140‑1 (MQ=255)                                                  cGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGatat                                                                 >  1:1653926/1‑140 (MQ=255)                                                  cGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGatat                                                                 >  2:965791/1‑140 (MQ=255)                                                      gCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAAt                                                             <  2:1218807/140‑1 (MQ=255)                                                      gCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAAt                                                             <  2:1653926/140‑1 (MQ=255)                                                       cTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATg                                                            <  2:1207553/140‑1 (MQ=255)                                                       cTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATg                                                            >  1:905144/1‑140 (MQ=255)                                                          ttttGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATc                                                         >  2:1290985/1‑140 (MQ=255)                                                            ttGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATccc                                                       <  2:1413640/140‑1 (MQ=255)                                                            ttGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATccc                                                       <  1:919879/140‑1 (MQ=255)                                                                ctggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCg                                                   <  1:1764653/140‑1 (MQ=255)                                                                ctggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCg                                                   <  1:1791189/140‑1 (MQ=255)                                                                ctggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCg                                                   <  2:752081/140‑1 (MQ=255)                                                                ctggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCg                                                   <  2:567447/140‑1 (MQ=255)                                                                ctggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCg                                                   <  1:375022/140‑1 (MQ=255)                                                                 tggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGc                                                  >  1:1483844/1‑140 (MQ=255)                                                                  ggctggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCt                                                 <  1:1464560/140‑1 (MQ=255)                                                                     tggTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTa                                              <  1:1179562/140‑1 (MQ=255)                                                                       gTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTAcg                                            <  1:1230468/140‑1 (MQ=255)                                                                       gTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTAcg                                            <  2:816218/140‑1 (MQ=255)                                                                          aGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGa                                         <  1:224039/140‑1 (MQ=255)                                                                                aacgaacTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGc                                   <  2:1819311/140‑1 (MQ=255)                                                                                aacgaacTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGc                                   <  1:297299/140‑1 (MQ=255)                                                                                 acgaacTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCa                                  >  2:1609845/1‑140 (MQ=255)                                                                                     acTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGc                              >  2:249267/1‑140 (MQ=255)                                                                                      cTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCt                             >  2:213909/1‑140 (MQ=255)                                                                                      cTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCt                             >  2:1833001/1‑140 (MQ=255)                                                                                        cAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTgg                           <  2:1265652/140‑1 (MQ=255)                                                                                        cAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTgg                           <  1:1290985/140‑1 (MQ=255)                                                                                         aaaGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGa                          <  1:838229/140‑1 (MQ=255)                                                                                             cTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTg                      <  2:785945/140‑1 (MQ=255)                                                                                                 cccGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGt                  >  2:663117/1‑140 (MQ=255)                                                                                                 cccGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGt                  >  1:510749/1‑140 (MQ=255)                                                                                                  ccGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTa                 <  2:1614450/140‑1 (MQ=255)                                                                                                     gCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTACgg              >  1:1193863/1‑140 (MQ=255)                                                                                                       gAAAGGTGTTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTACGgcg            <  2:510749/140‑1 (MQ=255)                                                                                                            gtgtTTCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTACGgcgcagac       <  2:17493/140‑5 (MQ=255)                                                                                                                 tCGGCACCGAAAGCGCCGCGTAATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTACGGCGCGGGTCTGCg  <  2:1411630/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                       CCGTAAAGGGCTGGGAGCAGCAAGTCTGGCGTTACGGCTTTCTGCGCTACGTAGCTTTTTTGACTGGCTGGTCAGCCAGAACGAACTCAAAGCTAACCCGGCGAAAGGTGTTTCGGCACCGAAAGCGCCGCGTCATCTGCCGAAAAACATCGACGTCGACGATATGAATCGGCTGCTGGATATTGATATCAATGATCCCCTCGCTGTACGCGACCGTGCAATGCTGGAAGTGATGTACGGCGCGGGTCTGCG  >  NC_000913/3996478‑3996729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑