Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,147,552	A→G	F256L (TTT→CTT)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,147,552	0	A	G	100.0%	282.7 / NA	77	F256L (TTT→CTT)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/40);  total (37/40)

GGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGG  >  NC_000913/4147416‑4147686                                                                                                                                         |                                                                                                                                       ggTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAg                                                                                                                                     <  1:1758908/140‑1 (MQ=255)  gTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGa                                                                                                                                    >  1:1212520/1‑140 (MQ=255)   tAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGAc                                                                                                                                   <  1:1577526/140‑1 (MQ=255)      tGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCa                                                                                                                                      <  1:673414/134‑1 (MQ=255)      tGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCa                                                                                                                                      >  2:673414/1‑134 (MQ=255)      tGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGc                                                                                                                                <  2:1600482/140‑1 (MQ=255)       gAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCa                                                                                                                               <  2:137100/140‑1 (MQ=255)            cgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAagt                                                                                                                          >  1:1288029/1‑140 (MQ=255)              gTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAagtag                                                                                                                        <  2:895923/140‑1 (MQ=255)               tGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAagtagt                                                                                                                       >  1:1478162/1‑140 (MQ=255)                 cgcAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTg                                                                                                                     >  1:26073/1‑140 (MQ=255)                  gcAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGc                                                                                                                                <  1:1357955/128‑1 (MQ=255)                  gcAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGc                                                                                                                                >  2:1357955/1‑128 (MQ=255)                         gCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGAc                                                                                                             <  1:359085/140‑1 (MQ=255)                          cTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACa                                                                                                            <  1:502306/140‑1 (MQ=255)                           tttCTGTGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACaa                                                                                                           >  1:1231242/1‑140 (MQ=255)                           tttCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACaa                                                                                                           >  2:1322270/1‑140 (MQ=255)                                gcgcCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCg                                                                                                      >  2:1823576/1‑140 (MQ=255)                                gcgcCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCg                                                                                                      >  2:1262780/1‑140 (MQ=255)                                gcgcCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCg                                                                                                      >  2:216625/1‑140 (MQ=255)                                   ccgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAg                                                                                                   >  1:1660288/1‑140 (MQ=255)                                            aCCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGcc                                                                                          <  1:1308080/140‑1 (MQ=255)                                            aCCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGcc                                                                                          <  1:431138/140‑1 (MQ=255)                                             cccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCa                                                                                         <  2:26073/140‑1 (MQ=255)                                             cccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCa                                                                                         <  1:38659/140‑1 (MQ=255)                                              ccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCAc                                                                                        <  1:1711553/140‑1 (MQ=255)                                              ccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCAc                                                                                        >  1:1093739/1‑140 (MQ=255)                                                 tCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtc                                                                                     >  1:889318/1‑140 (MQ=255)                                                    tATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCttt                                                                                  <  1:404695/140‑1 (MQ=255)                                                    tATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCttt                                                                                  <  1:650769/140‑1 (MQ=255)                                                     aTTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCtttt                                                                                 <  1:120194/140‑1 (MQ=255)                                                      ttCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt                                                                                   <  2:972598/137‑1 (MQ=255)                                                      ttCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt                                                                                   >  1:972598/1‑137 (MQ=255)                                                        cTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTACGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTAcc                                                                              >  2:531460/1‑140 (MQ=255)                                                          ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt                                                                            <  2:1660288/140‑1 (MQ=255)                                                           gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc                                                                           >  2:244202/1‑140 (MQ=255)                                                           gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc                                                                           <  2:1212520/140‑1 (MQ=255)                                                            agtagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCa                                                                          >  1:1574410/1‑140 (MQ=255)                                                             gtagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGTGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAg                                                                         >  1:641315/1‑140 (MQ=255)                                                              tagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAgg                                                                        >  1:73937/1‑140 (MQ=255)                                                                gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTc                                                                      >  2:1423817/1‑140 (MQ=255)                                                                gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTc                                                                      >  2:1536854/1‑140 (MQ=255)                                                                gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTc                                                                      >  2:1647026/1‑140 (MQ=255)                                                                gtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCAGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTc                                                                      >  1:592093/1‑140 (MQ=255)                                                                 tCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCa                                                                     >  2:1301155/1‑140 (MQ=255)                                                                  cGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCAt                                                                    <  1:1046130/140‑1 (MQ=255)                                                                  cGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCAt                                                                    <  1:216625/140‑1 (MQ=255)                                                                      ttttCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGcc                                                                <  2:1288029/140‑1 (MQ=255)                                                                       tttCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCt                                                               <  2:1814902/140‑1 (MQ=255)                                                                        ttCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCtt                                                              >  1:960852/1‑140 (MQ=255)                                                                              cAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                        <  2:1370330/140‑1 (MQ=255)                                                                              cAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                        <  2:1478162/140‑1 (MQ=255)                                                                              cAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                        <  1:1718152/140‑1 (MQ=255)                                                                              cAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATa                                                        <  1:1536854/140‑1 (MQ=255)                                                                                    gCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCt                                                  <  2:889318/140‑1 (MQ=255)                                                                                       gtgGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAg                                               <  1:430434/140‑1 (MQ=255)                                                                                         ggTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCg                                             <  1:1152530/140‑1 (MQ=255)                                                                                           tACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTg                                           <  1:244202/140‑1 (MQ=255)                                                                                                  ggCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGt                                    <  2:73937/140‑1 (MQ=255)                                                                                                  ggCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGt                                    >  2:1539930/1‑140 (MQ=255)                                                                                                   gCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCg                                             <  2:950412/130‑1 (MQ=255)                                                                                                   gCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCg                                             >  1:950412/1‑130 (MQ=255)                                                                                                   gCGATATTCCGACGGCGAGCGTTCGGCGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTc                                   >  1:1035464/1‑140 (MQ=255)                                                                                                    cGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTcg                                  >  2:1042291/1‑140 (MQ=255)                                                                                                     gATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTcgc                                 >  2:380831/1‑140 (MQ=255)                                                                                                        atTACGACGGCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCgtg                              <  1:531460/140‑1 (MQ=255)                                                                                                                 gCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCgtg                              >  1:701295/1‑131 (MQ=255)                                                                                                                 gCGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCgtg                              <  2:701295/131‑1 (MQ=255)                                                                                                                  cGAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGt                    >  1:325804/1‑140 (MQ=255)                                                                                                                   gAGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTa                   <  2:1093739/140‑1 (MQ=255)                                                                                                                    aGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTAt                  >  2:973049/1‑140 (MQ=255)                                                                                                                    aGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTAt                  <  1:747574/140‑1 (MQ=255)                                                                                                                    aGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTAt                  <  1:1539930/140‑1 (MQ=255)                                                                                                                    aGCGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTAt                  <  1:1423817/140‑1 (MQ=255)                                                                                                                      cGTTCGGTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTc                >  2:653968/1‑140 (MQ=255)                                                                                                                           ggTGTTTTTACGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTaa           >  1:682368/1‑140 (MQ=255)                                                                                                                                    aCGAAGCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATgg  >  2:518747/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCAGGTCATAGCCTTTCAATAACGTCTTAGCGTGCTCCAGTCGCGTGTGATTCAGGTATTCGTTAAAACCAATGG  >  NC_000913/4147416‑4147686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑