Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,201,514	T→C	S59G (AGC→GGC)	zraP ←	zinc responsive, periplasmic protein with chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,201,514	0	T	C	100.0%	300.9 / NA	81	S59G (AGC→GGC)	zraP	zinc responsive, periplasmic protein with chaperone activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/35);  total (46/35)

ACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGA  >  NC_000913/4201379‑4201646                                                                                                                                        |                                                                                                                                     aCTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGa                                                                                                                                  <  2:380808/140‑1 (MQ=255)    cATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCg                                                                                                                               >  2:114916/1‑140 (MQ=255)     aTCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGt                                                                                                                              >  1:1786102/1‑140 (MQ=255)      tCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTa                                                                                                                             >  2:1144157/1‑140 (MQ=255)      tCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTAAGCGTa                                                                                                                             >  1:644571/1‑140 (MQ=255)        tAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTaaa                                                                                                                           <  1:1046330/140‑1 (MQ=255)        tAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTaaa                                                                                                                           <  2:558165/140‑1 (MQ=255)          aCGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCctt                                                                                                                                    >  1:1717260/1‑129 (MQ=255)             aCTGACGCAAATTCTCGATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCa                                                                                                                      >  1:1212233/1‑140 (MQ=255)             aCTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCa                                                                                                                      >  1:1268984/1‑140 (MQ=255)               tGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGt                                                                                                                              <  1:1236690/130‑1 (MQ=255)               tGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGt                                                                                                                              >  2:1236690/1‑130 (MQ=255)               tGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCAtt                                                                                                                    >  1:1307785/1‑140 (MQ=255)               tGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTACTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCAtt                                                                                                                    >  2:647903/1‑140 (MQ=255)                 aCGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCGTAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTAt                                                                                                                  >  2:620573/1‑140 (MQ=255)                 aCGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTAt                                                                                                                  >  1:1271517/1‑140 (MQ=255)                 aCGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTAt                                                                                                                  >  2:141398/1‑140 (MQ=255)                 aCGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTAt                                                                                                                  >  1:568129/1‑140 (MQ=255)                 aCGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTAt                                                                                                                  >  1:752987/1‑140 (MQ=255)                  agagacatctcCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATg                                                                                                                 <  1:27908/133‑1 (MQ=255)                             cATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGcc                                                                                                      <  2:1174137/140‑1 (MQ=255)                             cATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGcc                                                                                                      >  1:1699081/1‑140 (MQ=255)                                ctctTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACg                                                                                                   <  1:114916/140‑1 (MQ=255)                                 tctTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGc                                                                                                  <  1:1583505/140‑1 (MQ=255)                                  ctTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGct                                                                                                 <  1:184913/140‑1 (MQ=255)                                  ctTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGct                                                                                                 <  1:1247096/140‑1 (MQ=255)                                   tttGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTT‑CTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGctgt                                                                                               >  2:414521/1‑140 (MQ=255)                                     tGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGctgtc                                                                                              >  1:1092619/1‑140 (MQ=255)                                          aCCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCtgttg                                                                                         >  2:643477/1‑140 (MQ=255)                                            cgcgTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTgttgtt                                                                                       >  1:536269/1‑140 (MQ=255)                                            cgcgTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTgttgtt                                                                                       <  1:1144157/140‑1 (MQ=255)                                              cgTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCg                                                                                     <  2:1212233/140‑1 (MQ=255)                                              cgTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCg                                                                                     <  2:1268984/140‑1 (MQ=255)                                              cgTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCg                                                                                     <  1:141398/140‑1 (MQ=255)                                              cgTTAATTTTGCTACTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCg                                                                                     <  1:647903/140‑1 (MQ=255)                                                      ttGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCaa                                                                             >  2:1616841/1‑140 (MQ=255)                                                             tATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGcgcg                                                                      <  1:643477/140‑1 (MQ=255)                                                              aTCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCgg                                                                     >  2:1023191/1‑140 (MQ=255)                                                                     gggTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtg                                                              <  2:1092619/140‑1 (MQ=255)                                                                     gggTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtg                                                              <  2:1826574/140‑1 (MQ=255)                                                                     gggTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtg                                                              <  2:1128284/140‑1 (MQ=255)                                                                      ggTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgc                                                             >  2:324892/1‑140 (MQ=255)                                                                      ggTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgc                                                             >  2:147031/1‑140 (MQ=255)                                                                       gTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgct                                                            <  1:790752/140‑1 (MQ=255)                                                                       gTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgct                                                            >  2:1607737/1‑140 (MQ=255)                                                                       gTTCGCGGCTAACAGGGCATTGTATTCATAACGCCTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgct                                                            <  2:797081/140‑1 (MQ=255)                                                                        ttCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTT‑CTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgctgc                                                          <  1:414521/140‑1 (MQ=255)                                                                         tCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgctgc                                                          >  2:1658589/1‑140 (MQ=255)                                                                         tCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgctgc                                                          >  2:901245/1‑140 (MQ=255)                                                                         tCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgctgc                                                          >  2:1368983/1‑140 (MQ=255)                                                                          cgcgGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGcc                                                         >  2:641974/1‑140 (MQ=255)                                                                           gcgGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCca                                                        >  2:175102/1‑140 (MQ=255)                                                                           gcgGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCca                                                        >  1:413370/1‑140 (MQ=255)                                                                            cgGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCcac                                                       <  2:1307785/140‑1 (MQ=255)                                                                                tAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCCCGCTGTCTTTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATAc                                                   <  2:398168/140‑1 (MQ=255)                                                                                         aTTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgct                                                            <  1:1750093/122‑1 (MQ=255)                                                                                         aTTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTtgct                                                            >  2:1750093/1‑122 (MQ=255)                                                                                         aTTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTccgc                                          >  2:1685712/1‑140 (MQ=255)                                                                                           tGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTccgccg                                        >  2:968944/1‑140 (MQ=255)                                                                                           tGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTccgccg                                        <  2:536269/140‑1 (MQ=255)                                                                                             tATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACTGTGTCCGCCGTg                                      <  1:1695827/140‑1 (MQ=255)                                                                                             tATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTg                                      >  2:944761/1‑140 (MQ=255)                                                                                                tCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGc                                   >  2:1100374/1‑140 (MQ=255)                                                                                                tCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGc                                   <  1:662286/140‑1 (MQ=255)                                                                                                tCATAACGCTTCGTCACCAGTTGCTGTTGCAGCGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGc                                   >  1:343791/1‑140 (MQ=255)                                                                                                 cATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCa                                  <  1:944761/140‑1 (MQ=255)                                                                                                  aTAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCaa                                 >  1:894876/1‑140 (MQ=255)                                                                                                    aaCGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAAt                               >  2:1464135/1‑140 (MQ=255)                                                                                                    aaCGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAAt                               >  2:13078/1‑140 (MQ=255)                                                                                                    aaCGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAAt                               >  2:457472/1‑140 (MQ=255)                                                                                                    aaCGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCACTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAAt                               >  1:365005/1‑140 (MQ=255)                                                                                                     aCGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATg                              >  1:314707/1‑140 (MQ=255)                                                                                                      cGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGc                             <  2:390091/140‑1 (MQ=255)                                                                                                       gCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCa                            <  1:1308516/140‑1 (MQ=255)                                                                                                       gCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCa                            <  2:1786102/140‑1 (MQ=255)                                                                                                         ttCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGa                          <  1:457472/140‑1 (MQ=255)                                                                                                         ttCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGa                          >  2:208587/1‑140 (MQ=255)                                                                                                          tCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAg                           <  1:796230/138‑1 (MQ=255)                                                                                                          tCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAg                           >  2:796230/1‑138 (MQ=255)                                                                                                          tCGTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGAt                         >  2:1065421/1‑140 (MQ=255)                                                                                                            gTCACCAGTTGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGt                       >  2:1388312/1‑140 (MQ=255)                                                                                                                    ttGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCa               >  2:880954/1‑140 (MQ=255)                                                                                                                     tGCTGTTGCAGTGCGCTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCAt              <  1:1651354/140‑1 (MQ=255)                                                                                                                                 gcgcTGCCTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGa  <  1:324892/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     ACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTGTCTGTTGTTCGCTGGTCAAAGGCGCGGCATTTTGCTGCCACATACCGTGTCCGCCGTGAGCAAATGCAGATGTCGATCCCATCGCCATTGCTGA  >  NC_000913/4201379‑4201646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑