Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,262,000	A→G	N111S (AAT→AGT)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,262,000	0	A	G	100.0%	271.6 / NA	73	N111S (AAT→AGT)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/32);  total (41/32)

TAAAGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCG  >  NC_000913/4261869‑4262132                                                                                                                                    |                                                                                                                                     tAAAGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCt                                                                                                                              >  1:446608/1‑140 (MQ=255) tAAAGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCt                                                                                                                              >  1:1582983/1‑140 (MQ=255)  aaaGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTg                                                                                                                             <  2:1634951/140‑1 (MQ=255)   aaGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGc                                                                                                                            <  1:947438/140‑1 (MQ=255)   aaGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGc                                                                                                                            >  2:693665/1‑140 (MQ=255)         aTTATCTGGCGTACCGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGTGATCAACCTGAGTGTCGCCTGCCCGTCt                                                                                                                      >  2:236426/1‑140 (MQ=255)         aTTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCt                                                                                                                      >  2:1829102/1‑140 (MQ=255)             tCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGTGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGAcc                                                                                                                  <  2:397330/140‑1 (MQ=255)             tCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGAcc                                                                                                                  <  2:960648/140‑1 (MQ=255)               tGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCgg                                                                                                                >  1:1120186/1‑140 (MQ=255)                ggCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCggg                                                                                                               >  2:519908/1‑140 (MQ=255)                ggCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCggg                                                                                                               >  2:179834/1‑140 (MQ=255)                      cAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGa                                                                                                         <  2:517484/140‑1 (MQ=255)                      cAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGa                                                                                                         <  1:239833/140‑1 (MQ=255)                       aGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGaa                                                                                                        <  1:1829102/140‑1 (MQ=255)                         tGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACg                                                                                                      >  1:1392495/1‑140 (MQ=255)                          gaagaagaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACgg                                                                                                     <  1:128243/140‑1 (MQ=255)                           aagaagaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGc                                                                                                    <  2:694760/140‑1 (MQ=255)                           aagaagaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGc                                                                                                    >  1:694760/1‑140 (MQ=255)                            agaagaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCa                                                                                                   <  2:446608/140‑1 (MQ=255)                              aagaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATg                                                                                                 >  2:1449411/1‑140 (MQ=255)                              aagaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATg                                                                                                 >  2:1045497/1‑140 (MQ=255)                                gaaCATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGGGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGtt                                                                                               <  2:647927/140‑1 (MQ=255)                                    aTCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGt                                                                                           <  1:699024/140‑1 (MQ=255)                                     tCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTg                                                                                          >  2:664864/1‑140 (MQ=255)                                     tCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTg                                                                                          >  2:1638255/1‑140 (MQ=255)                                     tCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTg                                                                                          >  1:646905/1‑140 (MQ=255)                                     tCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTg                                                                                          >  2:610177/1‑140 (MQ=255)                                       cGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCg                                                                                        <  2:81106/140‑1 (MQ=255)                                        ggTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCgt                                                                                       <  1:1638255/140‑1 (MQ=255)                                          tAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCgtgt                                                                                     <  1:111369/140‑1 (MQ=255)                                          tAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCgtgt                                                                                     <  2:1419126/140‑1 (MQ=255)                                          tAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCgtgt                                                                                     <  1:1182512/140‑1 (MQ=255)                                          tAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCgtgt                                                                                     <  1:664864/140‑1 (MQ=255)                                          tAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGAGATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCgtgt                                                                                     <  1:585150/140‑1 (MQ=255)                                                     cTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTaa                                                                          <  2:1582983/140‑1 (MQ=255)                                                        ggCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATgc                                                                       >  1:763252/1‑140 (MQ=255)                                                        ggCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATgc                                                                       >  2:1792409/1‑140 (MQ=255)                                                          cGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATgcgc                                                                     >  1:489647/1‑140 (MQ=255)                                                             tAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGc                                                                  >  1:720348/1‑140 (MQ=255)                                                                       gcggcgCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTTGGGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGa                                                        >  1:1750483/1‑140 (MQ=255)                                                                             cTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGt                                                  >  1:462759/1‑140 (MQ=255)                                                                                gcgcAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGaa                                               >  2:173270/1‑140 (MQ=255)                                                                                      tgtgCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGAt                                         <  2:1507237/140‑1 (MQ=255)                                                                                         gCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATgcg                                      <  1:202519/140‑1 (MQ=255)                                                                                         gCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATgcg                                      <  1:179834/140‑1 (MQ=255)                                                                                             aGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATgcg                                      <  1:1232104/136‑1 (MQ=255)                                                                                             aGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATgcg                                      >  2:1232104/1‑136 (MQ=255)                                                                                             aGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAt                                  >  1:1540557/1‑140 (MQ=255)                                                                                              gCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAtg                                 <  2:720348/140‑1 (MQ=255)                                                                                              gCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAtg                                 <  1:1792409/140‑1 (MQ=255)                                                                                               cTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAtgt                                <  1:1045497/140‑1 (MQ=255)                                                                                                tGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAtgtg                               >  2:812373/1‑140 (MQ=255)                                                                                                tGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAtgtg                               >  1:250536/1‑140 (MQ=255)                                                                                                tGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGAtgtg                               >  1:1353062/1‑140 (MQ=255)                                                                                                  gCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATgtggt                             >  2:1539919/1‑140 (MQ=255)                                                                                                  gCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATgtggt                             >  2:843927/1‑140 (MQ=255)                                                                                                  gCAGAAGCGCGCGGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATgtggt                             >  2:1179947/1‑140 (MQ=255)                                                                                                         cgcgcgGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGAtt                      >  1:1560839/1‑140 (MQ=255)                                                                                                            gcgGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCcg                   <  2:489647/140‑1 (MQ=255)                                                                                                            gcgGATATGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCcg                   <  2:706104/140‑1 (MQ=255)                                                                                                                atatGATGAGATCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCcggtga               <  2:646905/140‑1 (MQ=255)                                                                                                                       gagaTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGaa        <  1:1449411/140‑1 (MQ=255)                                                                                                                       gagaTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGaa        <  2:1392495/140‑1 (MQ=255)                                                                                                                         gaTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGaaaa      >  2:193045/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  1:1719485/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  2:261261/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  1:880994/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  2:739989/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  2:1354712/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  1:615204/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  2:933393/1‑140 (MQ=255)                                                                                                                          aTCAACCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGGTGCCGACTGCGTGAAAGTGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAc     >  2:162452/1‑140 (MQ=255)                                                                                                                             aaCCTGAGTGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAAcgcg  >  2:123521/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     TAAAGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGCGGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAATGTCGGCTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGAAAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCG  >  NC_000913/4261869‑4262132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑