BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,539	G→T	Q269H (CAG→CAT)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,539	0	G	T	100.0%	374.7 / NA	108	Q269H (CAG→CAT)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (58/50); total (58/50)

Only 100 of 114 total aligned reads displayed.

CTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCT  >  NC_000913/4631406‑4631673

cagaTACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  <  1:1269907/137‑1 (MQ=255)

cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  <  2:532250/140‑1 (MQ=255)

cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  <  2:1234929/140‑1 (MQ=255)

cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  >  1:1312339/1‑140 (MQ=255)

cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  <  1:613799/140‑1 (MQ=255)

cTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGACGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  <  1:189601/140‑1 (MQ=255)

     aCGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCt                                                                                                                             <  1:1452704/140‑1 (MQ=255)

       ggTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTa                                                                                                                           <  2:656316/140‑1 (MQ=255)

        gTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTaa                                                                                                                          >  1:1396243/1‑140 (MQ=255)

        gTACTGACCTTCACGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTaa                                                                                                                          >  1:110075/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGTGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  2:78103/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCgcag                                                                                                                                  <  1:1573700/131‑1 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  1:656968/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  1:59943/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  2:257585/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  1:1707209/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  2:1700852/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  2:1706052/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  2:470420/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  1:1414264/1‑140 (MQ=255)

         tACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAAt                                                                                                                         >  2:54489/1‑140 (MQ=255)

          aCTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATg                                                                                                                        >  2:1621736/1‑140 (MQ=255)

           cTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGa                                                                                                                       >  2:1059510/1‑140 (MQ=255)

            tGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGaa                                                                                                                      >  1:1142131/1‑140 (MQ=255)

             gACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGGTTCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGaaa                                                                                                                     >  2:1448727/1‑139 (MQ=255)

              aCCTTCGCGCGTACAACTGGCGCGACCGATTTTTCCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGa                                                                                                                    <  2:932114/140‑1 (MQ=255)

              aCCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGGAGGGTGCTGAGAATGCACGGCTGATGAACCCATCGCTTGCCCACGCGCAGCAGCTTAATGactt                                                                                                                    >  2:1269907/1‑137 (MQ=255)

              aCCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGa                                                                                                                    >  2:1666543/1‑140 (MQ=255)

               ccTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGAt                                                                                                                   >  2:931324/1‑140 (MQ=255)

                 ttCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGa                                                                                                                    >  1:1049798/1‑137 (MQ=255)

                 ttCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGa                                                                                                                    <  2:1049798/137‑1 (MQ=255)

                 ttCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCa                                                                                                                 <  2:1239869/140‑1 (MQ=255)

                 ttCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCa                                                                                                                 <  1:30168/140‑1 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGTGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  2:1509124/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGa                                                                                                                    <  2:74709/135‑1 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGa                                                                                                                    >  1:74709/1‑135 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGAt                                                                                                                   >  2:661348/1‑136 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  2:1805052/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  2:136724/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:1748319/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:1772908/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:166266/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  2:1795290/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:802699/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:664337/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:48797/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:537495/1‑140 (MQ=255)

                   cgcgcgTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGa                                                                                                               >  1:1808178/1‑140 (MQ=255)

                         tACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAgg                                                                                                         <  1:671328/140‑1 (MQ=255)

                         tACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAgg                                                                                                         <  1:931324/140‑1 (MQ=255)

                           cAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAg                                                                                                       <  2:537495/140‑1 (MQ=255)

                           cAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAg                                                                                                       >  1:664754/1‑140 (MQ=255)

                           cAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAg                                                                                                       <  2:1312339/140‑1 (MQ=255)

                                 gcgcGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTgcgc                                                                                                 >  1:1237915/1‑140 (MQ=255)

                                    cgACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGat                                                                                              >  2:1185344/1‑140 (MQ=255)

                                      aCCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGatat                                                                                            >  1:1350214/1‑140 (MQ=255)

                                      aCCGATTTTACCCGTCAAATGGCGGCGGTGGCG‑TTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATAtc                                                                                           >  1:1419790/1‑140 (MQ=255)

                                            tttACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGc                                                                                      <  1:1486655/140‑1 (MQ=255)

                                             ttACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGcc                                                                                     <  1:1783979/140‑1 (MQ=255)

                                              tACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCt                                                                                    <  2:1396243/140‑1 (MQ=255)

                                                   gTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCCGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGt                                                                               >  2:1326293/1‑140 (MQ=255)

                                                   gTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGt                                                                               <  1:1706052/140‑1 (MQ=255)

                                                    tCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGtt                                                                              >  1:1488344/1‑140 (MQ=255)

                                                       aaTGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGa                                                                           <  2:1808178/140‑1 (MQ=255)

                                                            cggcggTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGc                                                                      <  1:1621736/140‑1 (MQ=255)

                                                                ggTGGCGTTTGCCAGTGTGGTGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACg                                                                  <  1:1509124/140‑1 (MQ=255)

                                                                  tGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGAt                                                                >  2:530703/1‑140 (MQ=255)

                                                                         tGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACcg                                                         <  2:1707209/140‑1 (MQ=255)

                                                                            cAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACcgacg                                                      <  2:1419790/140‑1 (MQ=255)

                                                                             aGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACcgacga                                                     <  2:664754/140‑1 (MQ=255)

                                                                                gtgGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCa                                                  >  2:585979/1‑140 (MQ=255)

                                                                                    cgcgGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCg                                              >  1:617072/1‑140 (MQ=255)

                                                                                       ggCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGaaa                                           <  2:1008993/140‑1 (MQ=255)

                                                                                               gCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGa                                   <  2:1414264/140‑1 (MQ=255)

                                                                                               gCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGa                                   <  1:1185344/140‑1 (MQ=255)

                                                                                                   agaATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGAt                                  <  2:184652/137‑1 (MQ=255)

                                                                                                   agaATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGAt                                  >  1:184652/1‑137 (MQ=255)

                                                                                                    gaATGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACg                              <  2:1008376/140‑1 (MQ=255)

                                                                                                       tGCACGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCa                           >  1:79319/1‑140 (MQ=255)

                                                                                                          aCGGCTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTa                        >  2:1144530/1‑140 (MQ=255)

                                                                                                              cTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcg                    >  1:678981/1‑140 (MQ=255)

                                                                                                              cTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcg                    <  1:1799798/140‑1 (MQ=255)

                                                                                                              cTGATGATCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcg                    <  1:257585/140‑1 (MQ=255)

                                                                                                               tgatgaTCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcgc                   <  2:166266/140‑1 (MQ=255)

                                                                                                               tgatgaTCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATgcgc                   <  1:1795290/140‑1 (MQ=255)

                                                                                                                 atgatCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTc                 <  1:101970/140‑1 (MQ=255)

                                                                                                                  tgatCCCATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCg                <  1:1059510/140‑1 (MQ=255)

                                                                                                                      cccATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAAt            <  1:655219/140‑1 (MQ=255)

                                                                                                                        cATCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCt          >  2:1032483/1‑140 (MQ=255)

                                                                                                                         aTCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTa         >  2:1610663/1‑140 (MQ=255)

                                                                                                                         aTCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTa         <  2:59943/140‑1 (MQ=255)

                                                                                                                          tCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTAc        <  1:458479/140‑1 (MQ=255)

                                                                                                                          tCGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTAc        >  1:1816838/1‑140 (MQ=255)

                                                                                                                           cGCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACt       >  2:346060/1‑140 (MQ=255)

                                                                                                                            gCTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACtt      >  1:962506/1‑140 (MQ=255)

                                                                                                                             cTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATg                      >  1:1552140/1‑123 (MQ=255)

                                                                                                                             cTTGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATg                      <  2:1552140/123‑1 (MQ=255)

                                                                                                                               tGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGc   >  2:300480/1‑140 (MQ=255)

                                                                                                                               tGCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGc   >  2:1134212/1‑140 (MQ=255)

                                                                                                                                gCCCATGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCt  >  1:1435949/1‑140 (MQ=255)

CTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGTGGCGCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCT  >  NC_000913/4631406‑4631673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`