Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,424,579	Δ1 bp	noncoding (2205/2904 nt)	rrlD ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,424,579	0	T	.	100.0%	93.1 / NA	41	noncoding (2205/2904 nt)	rrlD	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/30);  total (11/30)

ACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTA  >  NC_000913/3424512‑3424703                                                                    |                                                                                                                             aCTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCt                                                     <  1:468058/140‑1 (MQ=11)  ctctTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTa                                                    <  2:572164/140‑1 (MQ=14)       tAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCt                                               >  1:1049548/1‑140 (MQ=17)          gaggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTccc                                            >  2:323905/1‑140 (MQ=17)          gaggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTccc                                            <  2:1208003/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAATGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  2:750150/140‑1 (MQ=16)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  2:717892/140‑1 (MQ=17)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  2:156251/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  1:946000/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  1:296409/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  2:847201/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  1:323905/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  1:457906/140‑1 (MQ=18)            ggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAc                                          <  1:409539/140‑1 (MQ=18)               gaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTa                                       >  1:635757/1‑140 (MQ=17)               gaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTa                                       >  1:113889/1‑140 (MQ=17)               gaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTa                                       <  1:127906/140‑1 (MQ=18)               gaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTa                                       >  1:197247/1‑140 (MQ=17)               gaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTa                                       <  1:261963/140‑1 (MQ=17)                aCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTAt                                      >  2:447658/1‑140 (MQ=17)                 ccGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATc                                     <  1:357701/140‑1 (MQ=18)                 ccGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATc                                     <  1:978577/140‑1 (MQ=18)                    ccccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTa                                  <  1:1091742/140‑1 (MQ=18)                    ccccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTa                                  <  1:200488/140‑1 (MQ=18)                    ccccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTa                                  >  1:82995/1‑140 (MQ=17)                             aaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAg                         <  1:122921/140‑1 (MQ=18)                                   ccaccaGACACTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCaa                   >  2:608527/1‑140 (MQ=17)                                       caGACACGTTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGtt               <  2:1178072/140‑1 (MQ=255)                                             cTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt         >  1:300041/1‑140 (MQ=17)                                             cTGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt         <  2:635757/140‑1 (MQ=18)                                              tGTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTc        >  1:406772/1‑140 (MQ=17)                                               gTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCCAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCTCCTATCCTACACATCAAGGCTCAATGTTCAGTGTCa       <  2:794729/140‑1 (MQ=255)                                               gTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCa       <  2:1093729/140‑1 (MQ=18)                                               gTCCGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCa       <  1:1130285/140‑1 (MQ=18)                                                 ccGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAg     <  1:853687/140‑1 (MQ=18)                                                 ccGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAg     >  1:811792/1‑140 (MQ=17)                                                 ccGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAg     <  1:396625/140‑1 (MQ=18)                                                  cGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAGGGCTCAATGTTCAGTGTCAAGc    <  1:823128/140‑1 (MQ=16)                                                  cGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGc    <  1:608527/140‑1 (MQ=18)                                                  cGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGc    <  2:912104/140‑1 (MQ=18)                                                  cGCAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGATCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGc    <  1:919315/140‑1 (MQ=12)                                                    cAACCCGGATTACGGG‑CAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta  <  2:197247/140‑1 (MQ=17)                                                                    |                                                                                                                             ACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTA  >  NC_000913/3424512‑3424703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑