Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	144,849	T→A	G91G (GGT→GGA)	yadI →	putative PTS enzyme IIA component YadI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,849	0	T	A	100.0%	236.1 / NA	66	G91G (GGT→GGA)	yadI	putative PTS enzyme IIA component YadI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (35/31);  total (35/31)

CATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAA  >  NC_000913/144714‑144977                                                                                                                                        |                                                                                                                                 cATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCa                                                                                                                              >  2:1059766/1‑140 (MQ=255)   tgatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGTGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACg                                                                                                                            <  2:1054352/140‑1 (MQ=255)   tgatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACg                                                                                                                            <  2:405481/140‑1 (MQ=255)    gatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGt                                                                                                                           <  2:628739/140‑1 (MQ=255)        tgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTAcc                                                                                                                       >  1:1007798/1‑140 (MQ=255)        tgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTAcc                                                                                                                       <  2:1007798/140‑1 (MQ=255)        tgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTAcc                                                                                                                       >  2:1189363/1‑140 (MQ=255)        tgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTAcc                                                                                                                       <  2:691317/140‑1 (MQ=255)           gATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGtt                                                                                                                    >  2:417906/1‑140 (MQ=255)             tGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTaa                                                                                                                  <  1:473555/140‑1 (MQ=255)                  tGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGaa                                                                                                             <  2:667466/140‑1 (MQ=255)                  tGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGaa                                                                                                             >  1:1179528/1‑140 (MQ=255)                       gAAGCGGACTCCGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAgat                                                                                                        >  2:1084935/1‑140 (MQ=255)                         aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAgatga                                                                                                      >  1:28347/1‑140 (MQ=255)                              aCTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCt                                                                                                 <  2:901763/140‑1 (MQ=255)                                     tGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                                          <  2:1031068/140‑1 (MQ=255)                                     tGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                                          <  1:1059766/140‑1 (MQ=255)                                     tGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTg                                                                                          >  1:437585/1‑140 (MQ=255)                                       aGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGaa                                                                                        <  1:411743/140‑1 (MQ=255)                                        gggTGTCATCATCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGaaa                                                                                       >  1:384282/1‑140 (MQ=255)                                            gtCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCAt                                                                                   >  1:1193611/1‑140 (MQ=255)                                               atcatcTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                                                <  2:384282/140‑1 (MQ=255)                                               aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                                                <  2:28347/140‑1 (MQ=255)                                               aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                                                <  1:263207/140‑1 (MQ=255)                                               aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAc                                                                                <  1:1084935/140‑1 (MQ=255)                                                   tcttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGt                                                                            >  2:204032/1‑140 (MQ=255)                                                    cttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGtt                                                                           >  1:765959/1‑140 (MQ=255)                                                            atatAGCCGGAGTGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGttt                                                                   >  1:754040/1‑140 (MQ=255)                                                            atatAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGttt                                                                   >  1:161727/1‑140 (MQ=255)                                                                 gCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGa                                                              <  1:441962/140‑1 (MQ=255)                                                                      aGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTa                                                         <  2:505794/140‑1 (MQ=255)                                                                       gcgcCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCg                                                           <  1:726932/137‑1 (MQ=255)                                                                       gcgcCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCg                                                           >  2:726932/1‑137 (MQ=255)                                                                       gcgcCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTAt                                                        >  2:903121/1‑140 (MQ=255)                                                                       gcgcCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTAt                                                        >  1:247015/1‑140 (MQ=255)                                                                              cGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGaa                                                 <  2:528419/140‑1 (MQ=255)                                                                                tATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACt                                               >  1:506017/1‑140 (MQ=255)                                                                                   cgcgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTggg                                            <  1:958894/140‑1 (MQ=255)                                                                                     cgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTgggag                                          >  1:478414/1‑138 (MQ=255)                                                                                     cgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTg                                          >  1:1036697/1‑140 (MQ=255)                                                                                     cgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTg                                          >  1:111813/1‑140 (MQ=255)                                                                                       tGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcg                                        >  2:202752/1‑140 (MQ=255)                                                                                       tGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcg                                        <  1:202752/140‑1 (MQ=255)                                                                                        ggCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                                       <  1:310065/140‑1 (MQ=255)                                                                                        ggCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgcgc                                       >  2:579570/1‑140 (MQ=255)                                                                                          cTTCATTATTAAGCCACAAACACTACCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCg                                     <  2:1179528/140‑1 (MQ=255)                                                                                             cATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAg                                  <  1:720919/140‑1 (MQ=255)                                                                                             cATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAg                                  <  1:417906/140‑1 (MQ=255)                                                                                              attattAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAgg                                 <  2:1077329/140‑1 (MQ=255)                                                                                              attattAAGCCACAAACACT‑CCGTTGCGAAGTGATTTCTGGAGTCACGTTTCCGTTAATTGAACAGATGATGGCTTGCCGTGAATCCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGt                                <  1:1180492/140‑1 (MQ=255)                                                                                                 attaAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGa                              <  1:1189363/140‑1 (MQ=255)                                                                                                     aGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGagtag                          >  1:699152/1‑140 (MQ=255)                                                                                                        cacaAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCt                       >  2:906926/1‑140 (MQ=255)                                                                                                        cacaAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCt                       >  2:888647/1‑140 (MQ=255)                                                                                                        cacaAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCt                       >  1:391879/1‑140 (MQ=255)                                                                                                         acaAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCtt                      >  1:466434/1‑140 (MQ=255)                                                                                                          caAACACTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCttt                     >  2:70836/1‑140 (MQ=255)                                                                                                                cTCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCAcc               <  1:888647/140‑1 (MQ=255)                                                                                                                 tCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGag                             >  2:420597/1‑125 (MQ=255)                                                                                                                 tCCCGTTGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGag                             <  1:420597/125‑1 (MQ=255)                                                                                                                      ttGCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACcaacaac         >  1:555302/1‑140 (MQ=255)                                                                                                                        gCGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACcaacaacaa       >  1:1067494/1‑140 (MQ=255)                                                                                                                         cGAAGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACaaa      >  1:454392/1‑140 (MQ=255)                                                                                                                           aaGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACaaaaa    >  1:409126/1‑140 (MQ=255)                                                                                                                           aaGTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACaaaaa    >  2:1061085/1‑140 (MQ=255)                                                                                                                             gTGATTTCTGGAGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACaaaaaaa  <  2:754040/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 CATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAAA  >  NC_000913/144714‑144977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑