Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,372,106	C→A	P66P (CCC→CCA)	ycjN →	putative ABC transporter periplasmic binding protein YcjN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,372,106	0	C	A	100.0%	229.5 / NA	65	P66P (CCC→CCA)	ycjN	putative ABC transporter periplasmic binding protein YcjN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/35);  total (30/35)

GTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCCGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTATCGATCGCAAAGCGGTTGCCA  >  NC_000913/1371970‑1372242                                                                                                                                         |                                                                                                                                         gTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTg                                                                                                                                       >  1:563891/1‑140 (MQ=255)  tAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTgg                                                                                                                                      <  1:1194390/140‑1 (MQ=255)  tAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTgg                                                                                                                                      <  1:439241/140‑1 (MQ=255)      gaagaaAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGgaaga                                                                                                                                  <  2:68658/140‑1 (MQ=255)          aaaaTAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGAt                                                                                                                              <  1:148303/140‑1 (MQ=255)          aaaaTAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGAt                                                                                                                              <  2:325804/140‑1 (MQ=255)          aaaaTAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGAt                                                                                                                              <  2:881460/140‑1 (MQ=255)          aaaaTAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGAt                                                                                                                              <  1:418298/140‑1 (MQ=255)          aaaaTAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTCGAAAAAGAAATCCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGAt                                                                                                                              <  2:511372/140‑1 (MQ=255)            aaTAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGATAAGCAAGTGCCAGTGGAAGAAGATGc                                                                                                                            >  1:263346/1‑140 (MQ=255)               aaaaCGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCta                                                                                                                         <  1:442325/140‑1 (MQ=255)                aaaCGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACAGTTAAGCAAGTGCCAGTGGAAGAAGATGCCtat                                                                                                                        <  2:890503/140‑1 (MQ=255)                  aCGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATaa                                                                                                                      <  2:813240/140‑1 (MQ=255)                      aTGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACt                                                                                                                  <  2:288844/140‑1 (MQ=255)                      aTGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACt                                                                                                                  <  2:63162/140‑1 (MQ=255)                      aTGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACt                                                                                                                  >  2:380286/1‑140 (MQ=255)                        gTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTaa                                                                                                                <  2:309896/140‑1 (MQ=255)                        gTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTaa                                                                                                                <  1:843792/140‑1 (MQ=255)                        gTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGACCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAATAAGATGCCTATAACACTaa                                                                                                                <  2:742647/140‑1 (MQ=255)                           tCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGt                                                                                                             >  2:175194/1‑140 (MQ=255)                           tCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGt                                                                                                             <  1:740957/140‑1 (MQ=255)                             cATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCa                                                                                                           >  1:70437/1‑140 (MQ=255)                               tCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCta                                                                                                                         >  2:731196/1‑124 (MQ=255)                               tCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCta                                                                                                                         <  1:731196/124‑1 (MQ=255)                                cGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTa                                                                                                        >  2:169584/1‑140 (MQ=255)                                cGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTa                                                                                                        >  2:84541/1‑140 (MQ=255)                                cGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCCATAACACTAAAGTCATTa                                                                                                        >  2:546441/1‑140 (MQ=255)                                  aaTTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTAct                                                                                                      <  1:169584/140‑1 (MQ=255)                                  aaTTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTAct                                                                                                      <  1:286294/140‑1 (MQ=255)                                    tttATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTActct                                                                                                    >  1:157505/1‑140 (MQ=255)                                     ttATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCtt                                                                                                   <  1:1104727/140‑1 (MQ=255)                                                  ggTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGtt                                                                                      >  2:917320/1‑140 (MQ=255)                                                     ggAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGc                                                                                   >  2:281033/1‑140 (MQ=255)                                                       aGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTg                                                                                 >  2:369425/1‑140 (MQ=255)                                                       aGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTg                                                                                 >  1:17980/1‑140 (MQ=255)                                                       aGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTg                                                                                 >  2:283999/1‑140 (MQ=255)                                                         cAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGc                                                                                   <  2:632484/136‑1 (MQ=255)                                                         cAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGc                                                                                   >  1:632484/1‑136 (MQ=255)                                                         cAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGcc                                                                               >  1:997001/1‑140 (MQ=255)                                                                  cAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGAt                                                                      <  1:582938/140‑1 (MQ=255)                                                                   aGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATc                                                                     <  1:1120127/140‑1 (MQ=255)                                                                       cGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGaaa                                                                 <  1:283999/140‑1 (MQ=255)                                                                       cGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGaaa                                                                 <  1:84541/140‑1 (MQ=255)                                                                       cGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGaaa                                                                 <  2:52154/140‑1 (MQ=255)                                                                       cGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATAACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGaaa                                                                 >  1:622616/1‑140 (MQ=255)                                                                               gTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCAt                                                         >  2:967747/1‑140 (MQ=255)                                                                                tAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATg                                                        >  2:1041286/1‑140 (MQ=255)                                                                                tAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATg                                                        >  2:1135529/1‑140 (MQ=255)                                                                                   attgattgCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACt                                                     >  1:78641/1‑140 (MQ=255)                                                                                   attgattgCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACt                                                     >  1:544775/1‑140 (MQ=255)                                                                                      gattgCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACg                                                  <  1:967747/140‑1 (MQ=255)                                                                                      gattgCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATAACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACg                                                  <  2:622616/140‑1 (MQ=255)                                                                                          gCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCaa                                              >  2:790584/1‑140 (MQ=255)                                                                                           cccGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTGCTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCaaa                                             <  2:263346/140‑1 (MQ=255)                                                                                           cccGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCaaa                                             <  2:503963/140‑1 (MQ=255)                                                                                           cccGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCaaa                                             >  1:294484/1‑140 (MQ=255)                                                                                              gTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTg                                          <  1:380286/140‑1 (MQ=255)                                                                                               ttttGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGa                                         >  2:9473/1‑140 (MQ=255)                                                                                                     aaaaGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACa                                   <  1:917320/140‑1 (MQ=255)                                                                                                     aaaaGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACa                                   <  2:17980/140‑1 (MQ=255)                                                                                                     aaaaGAAAACCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCCATAACACTAAAGTCATTACTCCTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACa                                   <  1:546441/140‑1 (MQ=255)                                                                                                           aaaCCCTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGagc                             <  1:281033/140‑1 (MQ=255)                                                                                                              cccTGGCATCACCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAGCATTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGAAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGTACAAAGagcagc                          <  2:563891/140‑1 (MQ=255)                                                                                                                        aCCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTatcgatcg                >  2:35410/1‑140 (MQ=255)                                                                                                                        aCCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTatcgatcg                >  2:899866/1‑140 (MQ=255)                                                                                                                        aCCGTTAAGCAAGTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTatcgatcg                >  2:927494/1‑140 (MQ=255)                                                                                                                                    gTGCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTATCGATCGCAAAGCGGTTGc    >  2:1175081/1‑140 (MQ=255)                                                                                                                                      gCCAGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTATCGATCGCAAAGCGGTAGCca  <  2:294484/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTAAAGAAGAAAATAAAACGAATGTATCCATCGAATTTATGCATTCTTCGGTGGAGCAGGAGCGCCAGGCCGTTATCAGTAAATTGATTGCCCGTTTTGAAAAAGAAAACCCTGGCATCACCGTTAAGCAAGTGCCCGTGGAAGAAGATGCCTATAACACTAAAGTCATTACTCTTTCACGTAGCGGTTCGCTGCCGGAAGTGATCGAAACCAGCCATGACTACGCCAAAGTGATGGACAAAGAGCAGCTTATCGATCGCAAAGCGGTTGCCA  >  NC_000913/1371970‑1372242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑