Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,064,892	T→A	T111S (ACC→TCC)	cobS ←	cobalamin 5'‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,064,892	0	T	A	100.0%	316.3 / NA	88	T111S (ACC→TCC)	cobS	cobalamin 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (38/50);  total (38/50)

AATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGTACGCTAAACAGT  >  NC_000913/2064756‑2065028                                                                                                                                         |                                                                                                                                         aataatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGcc                                                                                                                                       >  2:1200981/1‑140 (MQ=255)   taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTa                                                                                                                                     >  1:736821/1‑140 (MQ=255)   taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTa                                                                                                                                     >  2:742106/1‑140 (MQ=255)   taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTa                                                                                                                                     >  2:209937/1‑140 (MQ=255)     atGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTaaa                                                                                                                                   >  1:679730/1‑140 (MQ=255)      tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  1:670891/1‑140 (MQ=255)      tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  1:695761/1‑140 (MQ=255)      tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  2:23028/1‑140 (MQ=255)      tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  2:305819/1‑140 (MQ=255)      tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  2:402401/1‑140 (MQ=255)      tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  2:996952/1‑140 (MQ=255)      tGCGGCAGGCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  >  1:476744/1‑140 (MQ=255)      tGCGGAAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGGCACGCAGAGCCAGCTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAAc                                                                                                                                  <  2:648372/140‑1 (MQ=255)       gCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACg                                                                                                                                 <  1:222323/140‑1 (MQ=255)        cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGTCAGACCGCCGTGGGAGCCTAAACgg                                                                                                                                >  1:467998/1‑140 (MQ=255)        cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACgg                                                                                                                                >  1:1154814/1‑140 (MQ=255)        cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACgg                                                                                                                                <  1:74252/140‑1 (MQ=255)        cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACgg                                                                                                                                >  2:1182783/1‑140 (MQ=255)         ggCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGc                                                                                                                               <  2:16144/140‑1 (MQ=255)         ggCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGc                                                                                                                               >  1:251091/1‑140 (MQ=255)         ggCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGc                                                                                                                               <  2:256694/140‑1 (MQ=255)           cAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTa                                                                                                                             >  2:1123316/1‑140 (MQ=255)            aGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTAt                                                                                                                            <  1:233388/140‑1 (MQ=255)            aGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTAt                                                                                                                            <  2:961523/140‑1 (MQ=255)              tCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCa                                                                                                                          <  1:23028/140‑1 (MQ=255)              tCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCa                                                                                                                          <  1:402401/140‑1 (MQ=255)              tCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCa                                                                                                                          <  1:305819/140‑1 (MQ=255)              tCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCa                                                                                                                          >  2:998700/1‑140 (MQ=255)                 cgcgGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGc                                                                                                                       <  1:1197215/140‑1 (MQ=255)                   cgGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCAt                                                                                                                     <  1:375624/140‑1 (MQ=255)                   cgGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCAt                                                                                                                     <  1:225733/140‑1 (MQ=255)                        gACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGAttt                                                                                                                >  1:85243/1‑140 (MQ=255)                         aCCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTc                                                                                                               <  2:589926/140‑1 (MQ=255)                         aCCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTc                                                                                                               <  1:543278/140‑1 (MQ=255)                         aCCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTc                                                                                                               <  1:820980/140‑1 (MQ=255)                         aCCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTc                                                                                                               >  1:30136/1‑140 (MQ=255)                                       cgccAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATC‑TTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATcgcg                                                                                                <  2:588265/140‑1 (MQ=255)                                                tGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTg                                                                                        <  2:789212/140‑1 (MQ=255)                                                tGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTg                                                                                        >  2:1025854/1‑140 (MQ=255)                                                  cAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCa                                                                                      <  1:290562/140‑1 (MQ=255)                                                  cAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCa                                                                                      <  1:996952/140‑1 (MQ=255)                                                         cGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATa                                                                               <  1:742106/140‑1 (MQ=255)                                                           aTTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACg                                                                             <  1:998700/140‑1 (MQ=255)                                                           aTTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACg                                                                             >  1:652295/1‑140 (MQ=255)                                                             tCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGcc                                                                           <  2:1063765/140‑1 (MQ=255)                                                              cGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCa                                                                          <  1:46693/140‑1 (MQ=255)                                                                  aCGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCGACATGCGATCGCGGCTACGTGCAGAAAATACGCCATcac                                                                      <  1:1200981/140‑1 (MQ=255)                                                                    gCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAg                                                                    <  1:1025854/140‑1 (MQ=255)                                                                           cAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAg                                                             <  1:564377/140‑1 (MQ=255)                                                                              cTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCgg                                                          >  2:599548/1‑139 (MQ=255)                                                                              cTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGa                                                          >  2:697174/1‑140 (MQ=255)                                                                                  cTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGcc                                                      >  1:345730/1‑140 (MQ=255)                                                                                     aaCACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTc                                                   >  1:696032/1‑140 (MQ=255)                                                                                     aaCACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTc                                                   >  1:368562/1‑140 (MQ=255)                                                                                      acacCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTcc                                                  <  2:679730/140‑1 (MQ=255)                                                                                        acCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAg                                                <  2:92279/140‑1 (MQ=255)                                                                                        acCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAg                                                <  2:854947/140‑1 (MQ=255)                                                                                             aaTCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGa                                           <  2:881581/140‑1 (MQ=255)                                                                                             aaTCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGGATCAGCGAGGCCGTCCAGATGGa                                           >  1:482471/1‑140 (MQ=255)                                                                                                  ttGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATccc                                      >  1:1094653/1‑140 (MQ=255)                                                                                                   tGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATcccc                                     <  1:461404/140‑1 (MQ=255)                                                                                                    gCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATccccc                                    >  2:468134/1‑140 (MQ=255)                                                                                                    gCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATccccc                                    >  2:515881/1‑140 (MQ=255)                                                                                                     ccAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCg                                   <  2:1154814/140‑1 (MQ=255)                                                                                                     ccAGTACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCg                                   <  2:345730/140‑1 (MQ=255)                                                                                                         tACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGtca                               >  1:366993/1‑140 (MQ=255)                                                                                                         tACCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGtca                               >  1:1080587/1‑140 (MQ=255)                                                                                                          aCCACAAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGtcat                              >  2:1050820/1‑140 (MQ=255)                                                                                                             acaAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcag                           >  2:278990/1‑140 (MQ=255)                                                                                                              caAAAATCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagc                          >  2:237363/1‑140 (MQ=255)                                                                                                               aaaaaTCAATGTCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagca                         <  2:467998/140‑1 (MQ=255)                                                                                                                aaaaTCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagcac                        <  2:54948/140‑1 (MQ=255)                                                                                                                aaaaTCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagcac                        <  2:670891/140‑1 (MQ=255)                                                                                                                aaaaTCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagcac                        <  1:1050820/140‑1 (MQ=255)                                                                                                                aaaaTCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagcac                        <  1:209937/140‑1 (MQ=255)                                                                                                                   aTCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagcaccag                     <  2:30136/140‑1 (MQ=255)                                                                                                                   aTCAATGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATcagcaccag                     >  2:948448/1‑140 (MQ=255)                                                                                                                      aaTGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATAAGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCgg                                  <  2:221226/124‑1 (MQ=255)                                                                                                                      aaTGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATAAGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCgg                                  >  1:221226/1‑124 (MQ=255)                                                                                                                        tGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACaa                <  2:465452/140‑1 (MQ=255)                                                                                                                        tGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACaa                <  1:1123316/140‑1 (MQ=255)                                                                                                                        tGCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACaa                <  2:1080587/140‑1 (MQ=255)                                                                                                                         gCCAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAg               >  1:1086247/1‑140 (MQ=255)                                                                                                                          ccAGCCCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCGCCAGCACAAGt              <  1:1103507/140‑1 (MQ=255)                                                                                                                          ccAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGt              <  1:1182783/140‑1 (MQ=255)                                                                                                                          ccAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGt              <  2:736821/140‑1 (MQ=255)                                                                                                                          ccAGACCGCCGTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGt              <  2:476744/140‑1 (MQ=255)                                                                                                                                   cgTGGGAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGTACGCTAAAc     <  1:1034163/140‑1 (MQ=255)                                                                                                                                      gggAGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGTACGCTAAACAGt  <  1:697174/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGCTTAACACCAGAATCTTTGCCAGTACCACAAAAATCAATGCCAGACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGGTCATCAGCACCAGCACAAGTACGCTAAACAGT  >  NC_000913/2064756‑2065028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑