Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,563,831	A→C	I80I (ATA→ATC)	yffQ →	CPZ‑55 prophage; uncharacterized protein YffQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,563,831	0	A	C	100.0%	372.0 / NA	99	I80I (ATA→ATC)	yffQ	CPZ‑55 prophage; uncharacterized protein YffQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (47/52);  total (47/52)

GTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATAGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGTTTTGAG  >  NC_000913/2563697‑2563966                                                                                                                                       |                                                                                                                                        gTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGc                                                                                                                                    >  1:500392/1‑140 (MQ=255) gTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGc                                                                                                                                    >  1:1831/1‑140 (MQ=255)    gTTACTGATAACTGTGAAAAAACAGCCGTATTGGTGGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAAt                                                                                                                                 <  1:647067/140‑1 (MQ=255)    gTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAAt                                                                                                                                 <  1:87745/140‑1 (MQ=255)    gTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAAt                                                                                                                                 <  2:394487/140‑1 (MQ=255)    gTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAAt                                                                                                                                 <  1:431567/140‑1 (MQ=255)    gTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAAt                                                                                                                                 <  2:4396/140‑1 (MQ=255)    gTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGACTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAAt                                                                                                                                 <  1:485705/140‑1 (MQ=255)            tAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTa                                                                                                                         >  1:500177/1‑140 (MQ=255)                tgtgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAggg                                                                                                                     >  1:165785/1‑139 (MQ=255)                tgtgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGt                                                                                                                     >  1:953815/1‑140 (MQ=255)                tgtgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGt                                                                                                                     <  2:508136/140‑1 (MQ=255)                tgtgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGt                                                                                                                     >  1:510415/1‑140 (MQ=255)                tgtgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGt                                                                                                                     <  2:856582/140‑1 (MQ=255)                  tgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGa                                                                                                                   >  1:254857/1‑140 (MQ=255)                  tgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGa                                                                                                                   >  1:343021/1‑140 (MQ=255)                  tgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGa                                                                                                                   >  2:488526/1‑140 (MQ=255)                  tgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGa                                                                                                                   >  2:796155/1‑140 (MQ=255)                  tgAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGa                                                                                                                   >  2:847173/1‑140 (MQ=255)                    aaaaaaCAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACg                                                                                                                 <  2:659044/140‑1 (MQ=255)                    aaaaaaCAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACg                                                                                                                 >  2:1143992/1‑140 (MQ=255)                     aaaaaCAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGt                                                                                                                >  1:169901/1‑140 (MQ=255)                      aaaaCAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGtt                                                                                                               <  1:994363/140‑1 (MQ=255)                                      tAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACTTTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGaaa                                                                                               <  2:1111996/140‑1 (MQ=255)                                      tAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGaaa                                                                                               <  2:343021/140‑1 (MQ=255)                                      tAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGaaa                                                                                               <  2:500392/140‑1 (MQ=255)                                          cGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAgaga                                                                                           >  1:89963/1‑140 (MQ=255)                                          cGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCAGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAgaga                                                                                           >  2:475127/1‑140 (MQ=255)                                           ggTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGaa                                                                                          <  1:1098747/140‑1 (MQ=255)                                           ggTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCAGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGaa                                                                                          <  1:475127/140‑1 (MQ=255)                                               aCAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGaa                                                                                          <  1:971894/136‑1 (MQ=255)                                               aCAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGaa                                                                                          >  2:971894/1‑136 (MQ=255)                                                    cTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGa                                                                                 >  2:38370/1‑140 (MQ=255)                                                     ttGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGaa                                                                                <  2:254857/140‑1 (MQ=255)                                                       gCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGaaaa                                                                              >  2:135437/1‑140 (MQ=255)                                                       gCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGaaaa                                                                              >  1:411901/1‑140 (MQ=255)                                                       gCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGa                                                                                 >  1:559064/1‑137 (MQ=255)                                                       gCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGa                                                                                 <  2:559064/137‑1 (MQ=255)                                                             gTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTaa                                                                        >  2:756944/1‑140 (MQ=255)                                                              ttGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAAc                                                                       <  2:797930/140‑1 (MQ=255)                                                              ttGAGATCGAATATATAGCGACATTGGGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGAGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTagc                                                                       <  2:147322/140‑3 (MQ=255)                                                                 agaTCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTa                                                                    <  2:500177/140‑1 (MQ=255)                                                                 agaTCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTACTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTa                                                                    <  2:161798/140‑1 (MQ=255)                                                                     cGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGa                                                                >  2:459161/1‑140 (MQ=255)                                                                     cGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGa                                                                <  1:326939/140‑1 (MQ=255)                                                                      gAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGaa                                                               <  1:533853/140‑1 (MQ=255)                                                                       aaTATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAAc                                                              >  2:805973/1‑140 (MQ=255)                                                                       aaTATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAAc                                                              >  2:1086244/1‑140 (MQ=255)                                                                            ataGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGAt                                                         <  2:944211/140‑1 (MQ=255)                                                                              aGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATgg                                                       >  1:850838/1‑140 (MQ=255)                                                                              aGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATgg                                                       >  1:1141697/1‑140 (MQ=255)                                                                              aGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATgg                                                       >  2:94349/1‑140 (MQ=255)                                                                                cGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGaa                                                               <  1:767213/130‑1 (MQ=255)                                                                                cGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGaa                                                               >  2:767213/1‑130 (MQ=255)                                                                                  aCATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGa                                                   >  2:567840/1‑140 (MQ=255)                                                                                  aCATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGa                                                   >  2:24932/1‑140 (MQ=255)                                                                                   cATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAGGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGAt                                                  >  1:512392/1‑140 (MQ=255)                                                                                       gtgCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATctgg                                              <  1:1086244/140‑1 (MQ=255)                                                                                        tgCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATctggc                                             <  1:488526/140‑1 (MQ=255)                                                                                        tgCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATctggc                                             >  1:326669/1‑140 (MQ=255)                                                                                         gCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATctggct                                            <  2:909732/140‑1 (MQ=255)                                                                                            aGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGt                                         <  2:510415/140‑1 (MQ=255)                                                                                             gCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTc                                        >  1:546366/1‑140 (MQ=255)                                                                                              ccGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCg                                       <  1:756944/140‑1 (MQ=255)                                                                                               cGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGt                                      >  2:1096397/1‑140 (MQ=255)                                                                                                 gTGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTAt                                    <  1:94349/140‑1 (MQ=255)                                                                                                  tGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCg                                       <  1:89731/136‑1 (MQ=255)                                                                                                  tGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCg                                       >  2:89731/1‑136 (MQ=255)                                                                                                  tGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATc                                   >  2:768711/1‑140 (MQ=255)                                                                                                  tGGCTTTGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATc                                   >  2:1070239/1‑140 (MQ=255)                                                                                                      tttGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTg                               <  1:768711/140‑1 (MQ=255)                                                                                                        tGGTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCa                             <  1:179148/140‑1 (MQ=255)                                                                                                         ggTGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGCATCGTTGCag                            >  2:528517/1‑140 (MQ=255)                                                                                                           tGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGGAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCagag                          <  1:221915/140‑1 (MQ=255)                                                                                                           tGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCagag                          <  1:691603/140‑1 (MQ=255)                                                                                                           tGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCagag                          <  2:169901/140‑1 (MQ=255)                                                                                                           tGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCagag                          <  1:989747/140‑1 (MQ=255)                                                                                                           tGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCagag                          <  2:714403/140‑1 (MQ=255)                                                                                                           tGATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCagag                          <  1:972258/140‑1 (MQ=255)                                                                                                            gATTATTTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGc                         <  2:44694/140‑1 (MQ=255)                                                                                                              ttattTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGc                         <  2:114985/138‑1 (MQ=255)                                                                                                              ttattTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGc                         >  1:114985/1‑138 (MQ=255)                                                                                                              ttattTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAg                       >  1:470054/1‑140 (MQ=255)                                                                                                              ttattTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAg                       >  1:857616/1‑140 (MQ=255)                                                                                                               tattTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGt                      <  1:38370/140‑1 (MQ=255)                                                                                                                attTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCa                        >  1:1127305/1‑137 (MQ=255)                                                                                                                attTACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCa                        <  2:1127305/137‑1 (MQ=255)                                                                                                                  ttACGACAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTAtt                   <  1:1096397/140‑1 (MQ=255)                                                                                                                       aCAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGc              >  2:50567/1‑140 (MQ=255)                                                                                                                        cAGATTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCa             <  1:444262/140‑1 (MQ=255)                                                                                                                           aTTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGa          >  2:791395/1‑140 (MQ=255)                                                                                                                           aTTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGa          >  2:406431/1‑140 (MQ=255)                                                                                                                           aTTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGa          >  2:922938/1‑140 (MQ=255)                                                                                                                           aTTTGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGa          >  1:862069/1‑140 (MQ=255)                                                                                                                              tGACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGtt       <  2:1831/140‑1 (MQ=255)                                                                                                                               gACGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGttt      <  1:847818/140‑1 (MQ=255)                                                                                                                                aCGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGtttt     <  2:850838/140‑1 (MQ=255)                                                                                                                                aCGATATCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGtttt     <  2:857616/140‑1 (MQ=255)                                                                                                                                   atatCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGTTTTGAg  <  2:914117/140‑1 (MQ=255)                                                                                                                                   atatCGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGTTTTGAg  <  1:867893/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGCTTGCGAGGGTTGAGATCGAATATATAGCGACATTGTGCAAGCCGGTGGCTTTGGTGATTATTTACGACAGATTTGACGATATAGCGGCAATCCCTCTTAAGGTGACGTTGAAAAAGGTATTGAAAGAGAATAGCGATGAAAAGATTAACCTATTGAACAAGATGGGGGATCTGGCTGGTCGTATCGTTGCAGAGCAGTATTTAGGCATGAGTTTTGAG  >  NC_000913/2563697‑2563966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑