Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,594,365	C→A	E499* (GAG→TAG)	tmcA ←	tRNA(Met) cytidine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,594,365	0	C	A	100.0%	113.3 / NA	35	E499* (GAG→TAG)	tmcA	tRNA(Met) cytidine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/21);  total (14/21)

CTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGT  >  NC_000913/2594228‑2594495                                                                                                                                          |                                                                                                                                   cTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTacc                                                                                                                                  <  2:902291/140‑1 (MQ=255) cTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTacc                                                                                                                                  >  1:983882/1‑140 (MQ=255) cTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTacc                                                                                                                                  <  1:262009/140‑1 (MQ=255)           gccagcTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAAcc                                                                                                                        >  2:1092506/1‑140 (MQ=255)                 tGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACt                                                                                                                  <  1:383495/140‑1 (MQ=255)                                 tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCg                                                                                                  <  2:368759/140‑1 (MQ=255)                                 tCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCg                                                                                                  <  1:227997/140‑1 (MQ=255)                                   aTCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAg                                                                                                <  1:857408/140‑1 (MQ=255)                                        tAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCtt                                                                                           <  1:1024183/140‑1 (MQ=255)                                          aCAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGc                                                                                         <  2:991179/140‑1 (MQ=255)                                          aCAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGc                                                                                         >  2:128757/1‑140 (MQ=255)                                           cAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGCGTAACCAAAACTCACCGAAAGATAGTCGAGGCCTTGCg                                                                                        >  2:54705/1‑140 (MQ=255)                                                    cGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCa                                                                               <  1:1186370/140‑1 (MQ=255)                                                         tataGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCa                                                                          <  1:128757/140‑1 (MQ=255)                                                          ataGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCAc                                                                         <  1:401172/140‑1 (MQ=255)                                                           taGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCAcc                                                                        <  1:1092506/140‑1 (MQ=255)                                                           taGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCAc                                                                         <  2:1018826/139‑1 (MQ=255)                                                           taGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCAc                                                                         >  1:1018826/1‑139 (MQ=255)                                                                aCCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCaa                                                                   >  1:1186111/1‑140 (MQ=255)                                                                  cGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATa                                                                 <  1:434359/140‑1 (MQ=255)                                                                     tgctgGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGt                                                              >  2:763017/1‑140 (MQ=255)                                                                        tgGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTgc                                                           <  2:1186111/140‑1 (MQ=255)                                                                            ttCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTgccgcc                                                       <  1:196344/140‑1 (MQ=255)                                                                                 gatgatTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCtgtg                                                  >  2:536787/1‑140 (MQ=255)                                                                                        aCCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCt                                               <  1:383017/136‑1 (MQ=255)                                                                                        aCCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCt                                               >  2:383017/1‑136 (MQ=255)                                                                                        aCCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTccc                                           >  2:278656/1‑140 (MQ=255)                                                                                             tCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGa                                      <  2:378036/140‑1 (MQ=255)                                                                                              ccGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGAc                                     >  2:895932/1‑140 (MQ=255)                                                                                               cGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCTGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACg                                    >  1:325430/1‑140 (MQ=255)                                                                                                   ccAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAATCTCGCCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGcc                                <  2:983882/140‑1 (MQ=255)                                                                                                     aGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCgg                              <  1:536787/140‑1 (MQ=255)                                                                                                        acaAAACCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATg                           <  2:862547/140‑1 (MQ=255)                                                                                                           aaaCCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACTAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc                        <  2:469808/140‑1 (MQ=255)                                                                                                           aaaCCGCAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAAc                        <  1:278656/140‑1 (MQ=255)                                                                                                                 cAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTAt                  >  2:300551/1‑140 (MQ=255)                                                                                                                 cAGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTAt                  >  1:1106086/1‑140 (MQ=255)                                                                                                                  aGCGTTGCCAGAAACGCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATc                 >  2:439708/1‑140 (MQ=255)                                                                                                                                 gCCATAACTACCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGt  <  1:692978/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CTCACGTTCAGCCAGCTGTTTACCCGCATCACTCATCGGTAACAGCGCCATCGCCGTATAGCAACCGCTGCTGGCTTCCCGATGATTACCCATCCGCACCAGCACAAAACCGCAGCGTTGCCAGAAACGCCATAACTCCCCGGTGTAACCAAAACTCACCGAAAGATAGTCGAGGTCTTGCGTATATTGCAAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGT  >  NC_000913/2594228‑2594495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑